Adaptation of a High-Pressure Liquid Chromatography System for the Measurement of Viscosity
Abstract
:1. Introduction
2. Materials and Methods
2.1. High Pressure Liquid Chromatography (HPLC)
2.2. Materials
3. Results and Discussion
3.1. Optimization of Mobile Phase
3.2. Digital Removal of Pump Pulse Spikes
% (w/w) | B | D | η25 °C (cP) |
---|---|---|---|
0 | 64.526 | 0.096595 | 0.89 * |
10 | 64.526 | 0.094838 | 1.06 |
20 | 63.716 | 0.093106 | 1.42 |
30 | 62.710 | 0.090872 | 1.99 |
40 | 61.528 | 0.088231 | 2.95 |
50 | 60.012 | 0.084710 | 4.71 |
60 | 57.988 | 0.079863 | 8.41 |
65 | 56.804 | 0.077022 | 11.8 |
67 | 56.256 | 0.075692 | 13.7 |
70 | 55.355 | 0.073466 | 17.4 |
75 | 53.650 | 0.069230 | 27.0 |
80 | 51.654 | 0.064246 | 45.0 |
85 | 49.415 | 0.058684 | 80.4 |
90 | 46.630 | 0.051654 | 160 |
91 | 46.037 | 0.050186 | 187 |
92 | 45.333 | 0.048385 | 221 |
93 | 44.796 | 0.047127 | 260 |
94 | 44.206 | 0.045713 | 308 |
95 | 43.503 | 0.043944 | 367 |
96 | 42.864 | 0.042414 | 440 |
97 | 42.166 | 0.040700 | 531 |
98 | 41.435 | 0.038918 | 648 |
99 | 40.742 | 0.037274 | 794 |
100 | 40.075 | 0.035730 | 976 |
3.3. Measurement of Vaccine and Protein Samples
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
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Gregory, S.; Mach, H. Adaptation of a High-Pressure Liquid Chromatography System for the Measurement of Viscosity. Chromatography 2014, 1, 55-64. https://doi.org/10.3390/chromatography1020055
Gregory S, Mach H. Adaptation of a High-Pressure Liquid Chromatography System for the Measurement of Viscosity. Chromatography. 2014; 1(2):55-64. https://doi.org/10.3390/chromatography1020055
Chicago/Turabian StyleGregory, Sonia, and Henryk Mach. 2014. "Adaptation of a High-Pressure Liquid Chromatography System for the Measurement of Viscosity" Chromatography 1, no. 2: 55-64. https://doi.org/10.3390/chromatography1020055
APA StyleGregory, S., & Mach, H. (2014). Adaptation of a High-Pressure Liquid Chromatography System for the Measurement of Viscosity. Chromatography, 1(2), 55-64. https://doi.org/10.3390/chromatography1020055