Preparation of Rice Bran Protein (RBP) Powder Using Spray Drying Method at the Optimal Condition and Its Protein Quality
Abstract
:1. Introduction
2. Methodology
2.1. Raw Material
2.2. Extraction Process
2.3. Spray-Drying Process
- Wfinal (g) = Weight of the cylone after the spray-drying process;
- Winitial (g)= Weight of the cylone before the spray-drying process; and
- WRRB (g)= Weight of raw rice bran.
2.4. Other Drying Methods for Rice Bran Protein (RBP) Powder
2.5. Optimization of Spray-Drying Process Using Response Surface Methodology (RSM)
- A0 = Regression coefficients of variables for intercept terms;
- Ai = Regression coefficients of variables for linear terms;
- Aii = Regression coefficients of variables for quadratic terms;
- Aij = Regression coefficients of variables for interaction terms; and
- Xi, Xj = Independent variables.
2.6. Statistical Analysis
2.7. Model Validation
2.8. High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) for Protein Quality Analysis
2.9. Scanning Electron Microscope (SEM)
3. Result and Discussion
3.1. Effect of Inlet Temperature on Spray-Drying Process on RBP Powder Yield
3.2. Effect of Feed Flowrate on Spray-Drying Process on the RBP Powder Yield
3.3. Effect of Air Flowrate on Spray-Drying Process on the RBP Powder Yield
3.4. Optimization of the Spray-Drying Process for RBP Powder Production
- A = Inlet temperature (°C);
- B = Feed flowrate (%); and
- C = Air flowrate (L/h).
3.5. Comparison between Other Drying Methods
3.6. Protein Identification Analysis Based on Amino Acid Profiling
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Response (R1) | Experimental Data (g RBP/100 g RRB) | Predicted Value (g RBP/100 g RRB) | Error AARD (%) | |
---|---|---|---|---|
Average | AAD | |||
Yield | 25.66 | 0.55 | 24.9 | 2.96 |
Protein | Raw Rice Bran (RRB) | Rice Bran Protein (RBP) |
---|---|---|
Globulin | D | D |
Glutelin | D | D |
Prolamin | D | ND |
Glucose | ND | D |
Fructose | ND | D |
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Mansor, M.R.; Md Sarip, M.S.; Nik Daud, N.M.A.; Saidi, S.A.; Mohd Nawi, M.A.H.; Jamlos, M.A. Preparation of Rice Bran Protein (RBP) Powder Using Spray Drying Method at the Optimal Condition and Its Protein Quality. Processes 2022, 10, 2026. https://doi.org/10.3390/pr10102026
Mansor MR, Md Sarip MS, Nik Daud NMA, Saidi SA, Mohd Nawi MAH, Jamlos MA. Preparation of Rice Bran Protein (RBP) Powder Using Spray Drying Method at the Optimal Condition and Its Protein Quality. Processes. 2022; 10(10):2026. https://doi.org/10.3390/pr10102026
Chicago/Turabian StyleMansor, Mohd Rizuan, Mohd Sharizan Md Sarip, Nik Muhammad Azhar Nik Daud, Syahrul Affandi Saidi, Mohd Al Hafiz Mohd Nawi, and Mohd Aminudin Jamlos. 2022. "Preparation of Rice Bran Protein (RBP) Powder Using Spray Drying Method at the Optimal Condition and Its Protein Quality" Processes 10, no. 10: 2026. https://doi.org/10.3390/pr10102026