Neotropical Biodiversity as Microbial Frontline for Obtaining Bioactive Compounds with Potential Insecticidal Action
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling and Selection of Microorganisms
2.2. Isolation of Microorganisms
2.3. Submerged Fermentation
2.4. Application of Fermented Broth and Selection of Microorganisms
2.5. Lethal Concentrations
2.6. Analytical Procedure
Conidia Quantification
2.7. Identification of Microorganisms
2.8. Statistical Analysis
3. Results
3.1. Cultural and Phenotypic Characteristics of the Isolates
3.2. Selection of the Most Promising Isolates
3.3. Physicochemical Characteristics and PCA
3.4. Phylogenetic Tree
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Collection Location | State | Geographic Coordinates | Number of Microorganisms Collected | |
---|---|---|---|---|
Latitude | Longitude | |||
Santa Maria | RS | −29.4129 | −53.4803 | 125 |
Lavras do Sul | RS | −30.4857 | −53.5335 | 15 |
Cerro Largo | RS | −28.1506 | −54.7686 | 9 |
São Vicente do Sul | RS | −29.4132 | −54.4047 | 3 |
Luziânia | GO | −16.2345 | −47.9167 | 11 |
Microorganism | Identification | Classification | Collection Location | Concentration (Conidia mL−1) | Density (CFU) | Time (h) | Genre-Level Classification |
---|---|---|---|---|---|---|---|
1 | FT4.1.1 | Fungi | Santa Maria, RS, Brazil | 1.27 × 108 | 585.0 ± 71.0 | 48 | Talaromyces sp. |
2 | BR3 | Fungi | Luziânia, GO, Brazil | 5.10 × 106 | 1098.3 ± 336.5 | 96 | Aspergillus sp. |
3 | BR2.1 | Fungi | Luziânia, GO, Brazil | 3.00 × 108 | 922.6 ± 79.5 | 96 | Aspergillus sp. |
4 | MI2 | Fungi | Santa Maria, RS, Brazil | 2.30 × 106 | 127.0 ± 12.1 | 96 | Fusarium sp. |
5 | MI5 | Fungi | Santa Maria, RS, Brazil | 3.30 × 108 | 466.0 ± 0.0 | 72 | Talaromyces sp. |
6 | SH | Fungi | Santa Maria, RS, Brazil | 7.13 × 107 | 522.6 ± 147.0 | 96 | Penicillium sp. |
7 | MI3F2 | Fungi | Santa Maria, RS, Brazil | 3.20 × 108 | 612.3 ± 24.6 | 72 | Fusarium sp. |
8 | TR3.1 | Fungi | Santa Maria, RS, Brazil | 1.00 × 107 | 19.6 ± 7.3 | 96 | Fusarium sp. |
9 | C7 | Fungi | Santa Maria, RS, Brazil | 1.05 × 107 | 206.0 ± 27.0 | 120 | Talaromyces sp. |
10 | BR5.4 | Fungi | Luziânia, GO, Brazil | 3.30 × 107 | 214.6 ± 28.3 | 96 | Fusarium sp. |
11 | C9 | Fungi | Santa Maria, RS, Brazil | 7.00 × 108 | 231.6 ± 88.6 | 96 | Fusarium sp. |
12 | MI7 | Fungi | Santa Maria, RS, Brazil | 1.96 × 108 | 1194.0 ± 212.6 | 96 | Pythium sp. |
13 | OL1 | Fungi | Santa Maria, RS, Brazil | 1.40 × 107 | 81.6 ± 6.6 | 72 | Talaromyces sp. |
14 | CL4 | Fungi | Cerro Largo, RS, Brazil | 2.73 × 108 | 2.3 ± 1.3 | 48 | Trichoderma sp. |
15 | A6.1 | Fungi | Santa Maria, RS, Brazil | 2.10 × 107 | 3.0 ± 1.0 | 96 | Talaromyces sp. |
16 | MI3 | Fungi | Santa Maria, RS, Brazil | 6.00 × 108 | 42.0 ± 5.6 | 96 | Fusarium sp. |
17 | LV4 | Fungi | Lavras do Sul, RS, Brazil | 3.70 × 102 | 395.6 ± 88.5 | 96 | Trichoderma sp. |
18 | MIA | Fungi | Santa Maria, RS, Brazil | 7.10 × 107 | 267.0 ± 76.2 | 96 | Botryoderma sp. |
19 | SN5 | Fungi | Santa Maria, RS, Brazil | 2.40 × 108 | 31.6 ± 4.0 | 48 | Fusarium sp. |
20 | LV1 | Fungi | Lavras do Sul, RS, Brazil | 4.00 × 108 | 138.5 ± 44.5 | 96 | Trichoderma sp. |
21 | CL2 | Fungi | Cerro Largo, RS, Brazil | 6.10 × 108 | 200.0 ± 15.5 | 96 | Pythium sp. |
22 | BR4.1 | Fungi | Luziânia, GO, Brazil | 1.75 × 108 | 45.6 ± 23.0 | 96 | Nigrospora sp. |
23 | MI6 | Fungi | Santa Maria, RS, Brazil | 3.40 × 108 | 155.30 ± 71.60 | 96 | Aspergillus sp. |
24 | CL3 | Fungi | Cerro Largo, RS, Brazil | 1.00 × 108 | 13.00 ± 1.70 | 96 | Trichoderma sp. |
25 | OL4 | Fungi | Santa Maria, RS, Brazil | 2.10 × 108 | 271.00 ± 143.10 | 96 | Rhizoctonia sp. |
26 | MA8 | Fungi | Santa Maria, RS, Brazil | 2.00 × 108 | 674.30 ± 169.10 | 96 | Pythium sp. |
27 | FT4.1F | Fungi | Santa Maria, RS, Brazil | 1.71 × 108 | 118.00 ± 20.60 | 48 | Nigrospora sp. |
28 | LV12 | Fungi | Lavras do Sul, RS, Brazil | 1.90 × 107 | 2.00 ± 0.00 | 96 | Trichoderma sp. |
29 | LV7 | Fungi | Lavras do Sul, RS, Brazil | 2.30 × 108 | 1420.50 ± 47.30 | 96 | Penicillium sp. |
30 | MI7F2 | Fungi | Santa Maria, RS, Brazil | 1.05 × 108 | 154.60 ± 47.50 | 96 | Penicillium sp. |
31 | CL12 | Fungi | Cerro Largo, RS, Brazil | 3.50 × 108 | 2.00 ± 0.00 | 120 | Trichoderma sp. |
32 | SN4 | Fungi | Santa Maria, RS, Brazil | 5.77 × 108 | 584.00 ± 191.10 | 96 | Fusarium sp. |
33 | MI5F1 | Fungi | Santa Maria, RS, Brazil | 3.30 × 108 | 466.00 ± 0.00 | 72 | Trichoderma sp. |
34 | CL7 | Fungi | Cerro Largo, RS, Brazil | 7.60 × 108 | 571.00 ± 87.60 | 96 | Trichoderma sp. |
35 | SN9 | Fungi | Santa Maria, RS, Brazil | 4.33 × 107 | 31.30 ± 5.50 | 96 | Fusarium sp. |
36 | MI2.2 | Fungi | Santa Maria, RS, Brazil | 3.40 × 107 | 37.20 ± 5.50 | 96 | Fusarium sp. |
37 | P1 | Bacteria | Santa Maria, RS, Brazil | - | 620.00 ± 0.00 | 96 | - |
38 | CBM | Bacteria | Santa Maria, RS, Brazil | - | 3.60 ± 3.00 | 120 | - |
39 | X1 | Bacteria | Santa Maria, RS, Brazil | - | 290.60 ± 22.50 | 72 | - |
40 | MID | Bacteria | Santa Maria, RS, Brazil | - | 1109.60 ± 197.40 | 96 | - |
41 | UMB1 | Bacteria | São Vicente do Sul, RS, Brazil | - | 866.30 ± 106.80 | 96 | - |
42 | C1 | Bacteria | Santa Maria, RS, Brazil | - | 550.00 ± 86.20 | 48 | - |
43 | LV19 | Bacteria | Lavras do Sul, RS, Brazil | - | 61.00 ± 4.30 | 96 | - |
44 | UMB2 | Bacteria | São Vicente do Sul, RS, Brazil | - | 474.00 ± 80.90 | 48 | - |
45 | OL5 | Bacteria | Santa Maria, RS, Brazil | - | 138.50 ± 44.50 | 96 | - |
46 | FTG | Bacteria | Santa Maria, RS, Brazil | - | 31.30 ± 5.50 | 96 | - |
47 | BR7 | Bacteria | Luziânia, GO, Brazil | - | 51.00 ± 31.90 | 96 | - |
48 | UMB6 | Bacteria | São Vicente do Sul, RS, Brazil | - | 138.50 ± 44.50 | 48 | - |
49 | BR3.2 | Bacteria | Luziânia, GO, Brazil | - | 77.60 ± 25.50 | 48 | - |
50 | A8 | Bacteria | Santa Maria, RS, Brazil | - | 10.60 ± 0.50 | 96 | - |
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Santos, M.S.N.; Ody, L.P.; Kerber, B.D.; Castro, I.A.; de Villa, B.; Ugalde, G.A.; Guedes, J.V.C.; Mazutti, M.A.; Zabot, G.L.; Tres, M.V. Neotropical Biodiversity as Microbial Frontline for Obtaining Bioactive Compounds with Potential Insecticidal Action. Processes 2024, 12, 1722. https://doi.org/10.3390/pr12081722
Santos MSN, Ody LP, Kerber BD, Castro IA, de Villa B, Ugalde GA, Guedes JVC, Mazutti MA, Zabot GL, Tres MV. Neotropical Biodiversity as Microbial Frontline for Obtaining Bioactive Compounds with Potential Insecticidal Action. Processes. 2024; 12(8):1722. https://doi.org/10.3390/pr12081722
Chicago/Turabian StyleSantos, Maicon S. N., Lissara P. Ody, Bruno D. Kerber, Isac A. Castro, Bruna de Villa, Gustavo A. Ugalde, Jerson V. C. Guedes, Marcio A. Mazutti, Giovani L. Zabot, and Marcus V. Tres. 2024. "Neotropical Biodiversity as Microbial Frontline for Obtaining Bioactive Compounds with Potential Insecticidal Action" Processes 12, no. 8: 1722. https://doi.org/10.3390/pr12081722