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Volume 8
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10.3390/pr8010088
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Correction published on 15 April 2021, see Processes 2021, 9(4), 691.
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Article

A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation

by
Ilaria Roato
1,
Federico Mussano
1,
Simone Reano
2,
Filippo Boriani
3,
Andrea Margara
4,
Riccardo Ferracini
5,
Ezio Adriani
6,
Omar Sabry
7,*,
Mauro Fiorini
7 and
Paolo Fattori
7
1
CeRMS, A.O. Città della Salute e della Scienza, 10126 Torino, Italy
2
Department of Biochemistry, Muscle Regeneration Laboratory, University of Piemonte Orientale, 28100 Novara, Italy
3
Department of Plastic Surgery, Sanremo Hospital, 18038 Sanremo, Italy
4
Department of Plastic Surgey, Humanitas Cellini, 10126 Torino, Italy
5
Dipartimento di Scienze Chirurgiche, and Koelliker Hospital, Universitá di Genova, 10126 Torino, Italy
6
Unità Operativa Traumatologia dello Sport e Chirurgia del Ginocchio, Fondazione Universitaria Policlinico Gemelli IRCCS, 00168 Roma, Italy
7
Tiss’You, 47895 Domagnano, San Marino
*
Author to whom correspondence should be addressed.
Processes 2020, 8(1), 88; https://doi.org/10.3390/pr8010088
Submission received: 6 December 2019 / Revised: 19 December 2019 / Accepted: 28 December 2019 / Published: 9 January 2020
(This article belongs to the Special Issue Advances in Regenerative Medicine and Tissue Engineering)
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Abstract

This work aims to characterize a new method to recover low-manipulated human adipose tissue, enriched with adipose tissue-derived mesenchymal stem cells (ATD-MSCs) for autologous use in regenerative medicine applications. Lipoaspirated fat collected from patients was processed through Lipocell, a Class II-a medical device for dialysis of adipose tissue, by varying filter sizes and washing solutions. ATD-MSC yield was measured with flow cytometry after stromal vascular fraction (SVF) isolation in fresh and cultured samples. Purification from oil and blood was measured after centrifugation with spectrophotometer analysis. Extracellular matrix preservation was assessed through hematoxylin and eosin (H&E) staining and biochemical assay for total collagen, type-2 collagen, and glycosaminoglycans (GAGs) quantification. Flow cytometry showed a two-fold increase of ATD-MSC yield in treated samples in comparison with untreated lipoaspirate; no differences where reported when varying filter size. The association of dialysis and washing thoroughly removed blood and oil from samples. Tissue architecture and extracellular matrix integrity were unaltered after Lipocell processing. Dialysis procedure associated with Ringer’s lactate preserves the proliferation ability of ATD-MSCs in cell culture. The characterization of the product showed that Lipocell is an efficient method for purifying the tissue from undesired byproducts and preserving ATD-MSC vitality and extracellular matrix (ECM) integrity, resulting in a promising tool for regenerative medicine applications.
Keywords: adipose tissue; mesenchymal stem cells; regenerative medicine; Lipocell; extracellular matrix preservation; Ringer’s lactate; liposuction adipose tissue; mesenchymal stem cells; regenerative medicine; Lipocell; extracellular matrix preservation; Ringer’s lactate; liposuction
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MDPI and ACS Style

Roato, I.; Mussano, F.; Reano, S.; Boriani, F.; Margara, A.; Ferracini, R.; Adriani, E.; Sabry, O.; Fiorini, M.; Fattori, P. A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation. Processes 2020, 8, 88. https://doi.org/10.3390/pr8010088

AMA Style

Roato I, Mussano F, Reano S, Boriani F, Margara A, Ferracini R, Adriani E, Sabry O, Fiorini M, Fattori P. A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation. Processes. 2020; 8(1):88. https://doi.org/10.3390/pr8010088

Chicago/Turabian Style

Roato, Ilaria, Federico Mussano, Simone Reano, Filippo Boriani, Andrea Margara, Riccardo Ferracini, Ezio Adriani, Omar Sabry, Mauro Fiorini, and Paolo Fattori. 2020. "A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation" Processes 8, no. 1: 88. https://doi.org/10.3390/pr8010088

APA Style

Roato, I., Mussano, F., Reano, S., Boriani, F., Margara, A., Ferracini, R., Adriani, E., Sabry, O., Fiorini, M., & Fattori, P. (2020). A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation. Processes, 8(1), 88. https://doi.org/10.3390/pr8010088

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