Next Article in Journal
Fluid–Solid Coupling Model and Simulation of Gas-Bearing Coal for Energy Security and Sustainability
Next Article in Special Issue
Graphene Oxide as a Nanocarrier for Biochemical Molecules: Current Understanding and Trends
Previous Article in Journal
Triangulum City Dashboard: An Interactive Data Analytic Platform for Visualizing Smart City Performance
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Processes
Volume 8
Issue 2
10.3390/pr8020251
processes-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Size-Selected Graphene Oxide Loaded with Photosensitizer (TMPyP) for Targeting Photodynamic Therapy In Vitro

by
Kateřina Bartoň Tománková
1,
Ariana Opletalová
2,
Kateřina Poláková
2,*,
Sergii Kalytchuk
2,
Jana Jiravová
1,
Jakub Malohlava
1,
Lukáš Malina
1 and
Hana Kolářová
1
1
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Institute of Translational Medicine, Palacký University Olomouc, Hněvotínská 3, 775 15 Olomouc, Czech Republic
2
Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Šlechtitelů 27, 771 46 Olomouc, Czech Republic
*
Author to whom correspondence should be addressed.
Processes 2020, 8(2), 251; https://doi.org/10.3390/pr8020251
Submission received: 12 January 2020 / Revised: 12 February 2020 / Accepted: 17 February 2020 / Published: 24 February 2020
(This article belongs to the Special Issue Application of Carbon-Based Nanocomposites in Bioengineering and Biomedicine)
Browse Figures
Versions Notes

Abstract

Targeted therapies of various diseases are nowadays widely studied in many biomedical fields. Photodynamic therapy (PDT) represents a modern treatment of cancer using a locally activated light. TMPyP is an efficient synthetic water-soluble photosensitizer (PS), yet with poor absorption in the visible and the red regions. In this work, we prepared size-selected and colloidally stable graphene oxide (GO) that is appropriate for biomedical use. Thanks to the negative surface charge of GO, TMPyP was easily linked in order to create conjugates of GO/TMPyP by electrostatic force. Due to the strong ionic interactions, charge transfers between GO and TMPyP occur, as comprehensively investigated by steady-state and time-resolved fluorescence spectroscopy. Biocompatibility and an in vitro effect of GO/TMPyP were confirmed by a battery of in vitro tests including MTT, comet assay, reactive oxygen species (ROS) production, and monitoring the cellular uptake. PDT efficiency of GO/TMPyP was tested using 414 and 740 nm photoexcitation. Our newly prepared nanotherapeutics showed a higher PDT effect than in free TMPyP, and is promising for targeted therapy using clinically favorable conditions.
Keywords: size reduced graphene oxide; photosensitizer; TMPyP; in vitro; photodynamic therapy size reduced graphene oxide; photosensitizer; TMPyP; in vitro; photodynamic therapy
Graphical Abstract

Share and Cite

MDPI and ACS Style

Bartoň Tománková, K.; Opletalová, A.; Poláková, K.; Kalytchuk, S.; Jiravová, J.; Malohlava, J.; Malina, L.; Kolářová, H. Size-Selected Graphene Oxide Loaded with Photosensitizer (TMPyP) for Targeting Photodynamic Therapy In Vitro. Processes 2020, 8, 251. https://doi.org/10.3390/pr8020251

AMA Style

Bartoň Tománková K, Opletalová A, Poláková K, Kalytchuk S, Jiravová J, Malohlava J, Malina L, Kolářová H. Size-Selected Graphene Oxide Loaded with Photosensitizer (TMPyP) for Targeting Photodynamic Therapy In Vitro. Processes. 2020; 8(2):251. https://doi.org/10.3390/pr8020251

Chicago/Turabian Style

Bartoň Tománková, Kateřina, Ariana Opletalová, Kateřina Poláková, Sergii Kalytchuk, Jana Jiravová, Jakub Malohlava, Lukáš Malina, and Hana Kolářová. 2020. "Size-Selected Graphene Oxide Loaded with Photosensitizer (TMPyP) for Targeting Photodynamic Therapy In Vitro" Processes 8, no. 2: 251. https://doi.org/10.3390/pr8020251

APA Style

Bartoň Tománková, K., Opletalová, A., Poláková, K., Kalytchuk, S., Jiravová, J., Malohlava, J., Malina, L., & Kolářová, H. (2020). Size-Selected Graphene Oxide Loaded with Photosensitizer (TMPyP) for Targeting Photodynamic Therapy In Vitro. Processes, 8(2), 251. https://doi.org/10.3390/pr8020251

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop