Therapeutic Effect of Cinnamomum osmophloeum Leaf Extract on Oral Mucositis Model Rats Induced by 5-Fluororacil via Influencing IL-1β and IL-6 Levels
, Valendriyani Ningrum 1, Shih-Chieh Lee 2, Chi-Ting Li 3, Chang-Wei Hsieh 4, Sue-Hong Wang 3,5,* and Ming-Shun Tsai 2,*Round 1
Reviewer 1 Report
The authors studied the CO leaf extract affect for oral mucositis and revealed the relationship of pro-inflammatory cytokine level.
Normally, natural extract product has fewer side effects in the human.
Therefore, this research has high potential for therapeutic purpose.
To publication in Processes journal, manuscript need some scientifical modification as below.
- Dose CO have side effect report for human when they used high dose? If you have, add more information and reference papers in Introduction section.
- Body weight was significantly decreased in only treatment of CO 100mg/ml. How about only CO 50mg/ml? If body weight was not big difference between WT and CO 50 only, CO 50 is best condition for this research.
- Result 3.3 Food and water intake part (line 119 to 220). Line 200 to 220 sentences need rearrange. Explain of Fig3A, and 3B was changed. Authors should correct the 3.3 result section to the correct explanation.
- Fig 6. Although authors remarked 5uM in left side of image, this is an unusual mark for scale bar. I recommend put 5 um of scale bar in the image.
- Result 3.7 is main mechanism for CO in this research. However, Result 3.7 section deficient the conclusion for Fig7. Although authors explained relation of IL-1b and TNF-1 in discussion part, to understand this result more easily, author should add the meaning of the result in section 3.7.
- IACUC approved number for animal experiment should be need in material and method section.
Author Response
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Author Response File: 
Author Response.docx
Reviewer 2 Report
The manuscript presents an interesting and important health issue related to oral mucositis management. This manuscript is well written and organized, I highly appreciate grafic presentation of the results. However, I would like to suggest the following points for the betterment of this manuscript:
1.This a well designed study however very small groups are included in this research, which is the reason why I suggest to change the title and add „a pilot study”. Although the results are very promising, further research on a larger group is required and this information should be emphasized and mentioned not only in the main text, but also in abstract section.
2. The introduction section should be improved and include more specific information about oral mucosa clinical changes in those 5 stages of oral mucositis development, especially that this information is used in the results section and required to understand the results properly.
3. Photographs of oral mucosa: only 1 photograph in every group is presented. Was oral mucosa of all rats in one group the same? Was there any pattern to select photographs?
4. Results - clinical appearance: in lines 299 and 300 term „some rats” is used. I suggest to change it into a number of rats.
5. Statistical significance: in material and methods section only p<0.05 is mentioned, whereas in results of pro-inflammatory cytokines (Figure 7) there is also p<0.01 and p>0.001. I suggest to mention this information also in material and methods section.
Author Response
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Author Response File: Author Response.docx
