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Volume 8
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10.3390/separations8060075
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Communication

Heterologous Biosynthesis of Artemisinin in Chrysanthemum morifolium Ramat

by
Aleksey Firsov
1,*,
Alexander Pushin
1,
Svetlana Motyleva
2,
Svetlana Pigoleva
1,
Lyubov Shaloiko
1,
Alexander Vainstein
3 and
Sergey Dolgov
1
1
Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Prospekt Nauki, 6, Pushchino, 142290 Moscow Region, Russia
2
Federal Scientific Selection and Technology Center for Horticulture and Nursery, Zagoryevskaya St. 4, 115598 Moscow, Russia
3
Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 76100, Israel
*
Author to whom correspondence should be addressed.
Separations 2021, 8(6), 75; https://doi.org/10.3390/separations8060075
Submission received: 19 April 2021 / Revised: 20 May 2021 / Accepted: 26 May 2021 / Published: 28 May 2021
(This article belongs to the Section Analysis of Natural Products and Pharmaceuticals)
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Abstract

Artemisinin-based drugs are the most effective medicine against multidrug-resistant Plasmodium spp., the parasite that causes malaria. To this day, wormwood A. annua L. is the sole commercial source of artemisinin, where it is produced in minor amounts. The artemisinin yield depends on numerous poorly regulated agricultural factors and the genetic variability of this non-domesticated plant. This has aroused significant interest in the development of heterologous expression platforms for artemisinin production. Previously, we obtained lines of Chrysanthemum morifolium Ramat. (C. morifolium Ramat.), cvs. White Snowdon and Egyptianka, transformed with artemisinin biosynthesis genes. Here, we report the results of an analysis of artemisinin production in transgenic chrysanthemums. Transcription of heterologous amorpha-4,11-diene monooxygenase and cytochrome P450 reductase genes in transgenic lines was confirmed using high-resolution melting analysis. Artemisinin accumulation was detected using GC-MS in White Snowdon plants, but not in Egyptianka ones, thereby demonstrating the possibility of transplanting active artemisinin biosynthetic pathway into chrysanthemum. Ways of increasing its content in producer plants are discussed.
Keywords: plant biotechnology; malaria; heterologous biosynthesis; artemisinin; Chrysanthemum morifolium; plant metabolic engineering plant biotechnology; malaria; heterologous biosynthesis; artemisinin; Chrysanthemum morifolium; plant metabolic engineering

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MDPI and ACS Style

Firsov, A.; Pushin, A.; Motyleva, S.; Pigoleva, S.; Shaloiko, L.; Vainstein, A.; Dolgov, S. Heterologous Biosynthesis of Artemisinin in Chrysanthemum morifolium Ramat. Separations 2021, 8, 75. https://doi.org/10.3390/separations8060075

AMA Style

Firsov A, Pushin A, Motyleva S, Pigoleva S, Shaloiko L, Vainstein A, Dolgov S. Heterologous Biosynthesis of Artemisinin in Chrysanthemum morifolium Ramat. Separations. 2021; 8(6):75. https://doi.org/10.3390/separations8060075

Chicago/Turabian Style

Firsov, Aleksey, Alexander Pushin, Svetlana Motyleva, Svetlana Pigoleva, Lyubov Shaloiko, Alexander Vainstein, and Sergey Dolgov. 2021. "Heterologous Biosynthesis of Artemisinin in Chrysanthemum morifolium Ramat" Separations 8, no. 6: 75. https://doi.org/10.3390/separations8060075

APA Style

Firsov, A., Pushin, A., Motyleva, S., Pigoleva, S., Shaloiko, L., Vainstein, A., & Dolgov, S. (2021). Heterologous Biosynthesis of Artemisinin in Chrysanthemum morifolium Ramat. Separations, 8(6), 75. https://doi.org/10.3390/separations8060075

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