An Evaluation of the Accuracy of the Subtraction Method Used for Determining Platelet Counts in Advanced Platelet-Rich Fibrin and Concentrated Growth Factor Preparations
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. PRP and PRGF Preparation
4.2. Simulation of A-PRF and CGF Preparation in the Presence of the Anti-Coagulant
4.3. Preparation of A-PRF and CGF in the Absence of the Anti-Coagulant
4.4. Determination of Blood Cell Counts
4.5. Scanning Electron Microscopy (SEM)
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Conflicts of Interest
References
- Marx, R.E.; Carlson, E.R.; Eichstaedt, R.M.; Schimmele, S.R.; Strauss, J.E.; Georgeff, K.R. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endodontol. 1998, 85, 638–646. [Google Scholar] [CrossRef]
- Castro, A.B.; Meschi, N.; Temmerman, A.; Pinto, N.; Lambrechts, P.; Teughels, W.; Quirynen, M. Regenerative potential of Leucocyte- and Platelet Rich Fibrin (L-PRF). Part B: Sinus floor elevation, alveolar ridge preservation, and implant therapy. A systematic review. J. Clin. Periodontol. 2016. [Google Scholar] [CrossRef]
- Castro, A.B.; Meschi, N.; Temmerman, A.; Pinto, N.; Lambrechts, P.; Teughels, W.; Quirynen, M. Regenerative potential of leucocyte- and platelet-rich fibrin. Part A: Intra-bony defects, furcation defects and periodontal plastic surgery. A systematic review and meta-analysis. J. Clin. Periodontol. 2016. [Google Scholar] [CrossRef] [PubMed]
- Hehn, J.; Schwenk, T.; Striegel, M.; Schlee, M. The effect of PRF (platelet-rich fibrin) inserted with a split-flap technique on soft tissue thickening and initial marginal bone loss around implants: Results of a randomized, controlled clinical trial. Int. J. Implant Dent. 2016, 2. [Google Scholar] [CrossRef] [PubMed]
- Okuda, K.; Nakajima, Y.; Kawase, T.; Kobayashi, M.; Kamiya, M.; Wolff, L.; Yoshie, H. Platelet-rich fibrin membrane combined with beta-tricalcium phosphate for treatment of infrabony defects in chronic periodontitis: A case series. Jacobs J. Dent. Res. 2015, 2. [Google Scholar] [CrossRef]
- Dohan Ehrenfest, D.M.; Del Corso, M.; Diss, A.; Mouhyi, J.; Charrier, J.B. Three-dimensional architecture and cell composition of a Choukroun’s platelet-rich fibrin clot and membrane. J. Periodontol. 2010, 81, 546–555. [Google Scholar] [CrossRef] [PubMed]
- Aggarwal, A.; Singhal, N. Evaluation of content and distribution of platelets in platelet rich fibrin at various centrifugation time periods: A light microscopic study. Int. J. Dent. Med. Res. 2015, 1, 61–64. [Google Scholar]
- Eren, G.; Gurkan, A.; Atmaca, H.; Donmez, A.; Atilla, G. Effect of centrifugation time on growth factor and MMP release of an experimental platelet-rich fibrin-type product. Platelets 2016, 27, 427–432. [Google Scholar] [CrossRef] [PubMed]
- Peck, M.; Hiss, D.; Stephen, L.; Satti, A.; Majeed, A. Platelet-rich fibrin (PRF)—The effect of storage time on platelet concetration. South Afr. Dent. J. 2015, 70, 448–451. [Google Scholar]
- Radrizzani, M.; Lo Cicero, V.; Soncin, S.; Bolis, S.; Surder, D.; Torre, T.; Siclari, F.; Moccetti, T.; Vassalli, G.; Turchetto, L. Bone marrow-derived cells for cardiovascular cell therapy: An optimized GMP method based on low-density gradient improves cell purity and function. J. Transl. Med. 2014, 12. [Google Scholar] [CrossRef] [PubMed]
- Rayment, E.A.; Williams, D.J. Concise review: Mind the gap: Challenges in characterizing and quantifying cell- and tissue-based therapies for clinical translation. Stem Cells 2010, 28, 996–1004. [Google Scholar] [PubMed]
- McKiel, R.R.; Barron, N.; Needham, C.J.; Wilkins, T.A. Siliconized vs nonsiliconized evaluated blood-collection tubes for free thyroxin measurements. Clin. Chem. 1982, 28, 2333–2334. [Google Scholar] [PubMed]
- Kobayashi, M.; Kawase, T.; Horimizu, M.; Okuda, K.; Wolff, L.F.; Yoshie, H. A proposed protocol for the standardized preparation of PRF membranes for clinical use. Biologicals 2012, 40, 323–329. [Google Scholar] [CrossRef] [PubMed]
- Hermand, P.; Gane, P.; Huet, M.; Jallu, V.; Kaplan, C.; Sonneborn, H.H.; Cartron, J.P.; Bailly, P. Red cell ICAM-4 is a novel ligand for platelet-activated alpha IIbbeta 3 integrin. J. Biol. Chem. 2003, 278, 4892–4898. [Google Scholar] [CrossRef] [PubMed]
- Swanepoel, A.C.; Pretorius, E. Erythrocyte-platelet interaction in uncomplicated pregnancy. Microsc. Microanal. 2014, 20, 1848–1860. [Google Scholar] [CrossRef] [PubMed]
- Kawase, T. Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: Basic principles and concepts underlying recent advances. Odontology 2015, 103, 126–135. [Google Scholar] [CrossRef] [PubMed]
- Nakajima, Y.; Kawase, T.; Kobayashi, M.; Okuda, K.; Wolff, L.F.; Yoshie, H. Bioactivity of freeze-dried platelet-rich plasma in an adsorbed form on a biodegradable polymer material. Platelets 2012, 23, 594–603. [Google Scholar] [CrossRef] [PubMed]
- Okuda, K.; Kawase, T.; Momose, M.; Murata, M.; Saito, Y.; Suzuki, H.; Wolff, L.F.; Yoshie, H. Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-beta and modulates the proliferation of periodontally related cells in vitro. J. Periodontol. 2003, 74, 849–857. [Google Scholar] [CrossRef] [PubMed]
- Anitua, E. Plasma rich in growth factors: Preliminary results of use in the preparation of future sites for implants. Int. J. Oral Maxillofac. Implants 1999, 14, 529–535. [Google Scholar] [PubMed]
- Kobayashi, M.; Kawase, T.; Okuda, K.; Wolff, L.F.; Yoshie, H. In vitro immunological and biological evaluations of the angiogenic potential of platelet-rich fibrin preparations: A standardized comparison with PRP preparations. Int. J. Implant Dent. 2015, 1. [Google Scholar] [CrossRef] [PubMed]
- Masuki, H.; Okudera, T.; Watanebe, T.; Suzuki, M.; Nishiyama, K.; Okudera, H.; Nakata, K.; Uematsu, K.; Su, C.Y.; Kawase, T. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int. J. Implant Dent. 2016, 2. [Google Scholar] [CrossRef] [PubMed]
- Kawase, T.; Tanaka, T.; Nishimoto, T.; Okuda, K.; Nagata, M.; Burns, D.M.; Yoshie, H. Improved adhesion of human cultured periosteal sheets to a porous poly(L-lactic acid) membrane scaffold without the aid of exogenous adhesion biomolecules. J. Biomed. Mater. Res. A 2011, 98, 100–113. [Google Scholar] [CrossRef] [PubMed]
- Horimizu, M.; Kawase, T.; Nakajima, Y.; Okuda, K.; Nagata, M.; Wolff, L.F.; Yoshie, H. An improved freeze-dried PRP-coated biodegradable material suitable for connective tissue regenerative therapy. Cryobiology 2013, 66, 223–232. [Google Scholar] [CrossRef] [PubMed]
Centrifugation | PRP | PRGF | A-PRF | CGF |
---|---|---|---|---|
Force (g) | 1100 | 580 | 200 | 692 |
547 | ||||
592 | ||||
855 | ||||
Duration (min) | 8 | 8 | 8 | 2 |
4 | ||||
4 | ||||
3 | ||||
Appearance of ACD-A-contained blood samples | |
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Watanabe, T.; Isobe, K.; Suzuki, T.; Kawabata, H.; Nakamura, M.; Tsukioka, T.; Okudera, T.; Okudera, H.; Uematsu, K.; Okuda, K.; et al. An Evaluation of the Accuracy of the Subtraction Method Used for Determining Platelet Counts in Advanced Platelet-Rich Fibrin and Concentrated Growth Factor Preparations. Dent. J. 2017, 5, 7. https://doi.org/10.3390/dj5010007
Watanabe T, Isobe K, Suzuki T, Kawabata H, Nakamura M, Tsukioka T, Okudera T, Okudera H, Uematsu K, Okuda K, et al. An Evaluation of the Accuracy of the Subtraction Method Used for Determining Platelet Counts in Advanced Platelet-Rich Fibrin and Concentrated Growth Factor Preparations. Dentistry Journal. 2017; 5(1):7. https://doi.org/10.3390/dj5010007
Chicago/Turabian StyleWatanabe, Taisuke, Kazushige Isobe, Taiji Suzuki, Hideo Kawabata, Masayuki Nakamura, Tsuneyuki Tsukioka, Toshimitsu Okudera, Hajime Okudera, Kohya Uematsu, Kazuhiro Okuda, and et al. 2017. "An Evaluation of the Accuracy of the Subtraction Method Used for Determining Platelet Counts in Advanced Platelet-Rich Fibrin and Concentrated Growth Factor Preparations" Dentistry Journal 5, no. 1: 7. https://doi.org/10.3390/dj5010007