Angiogenic Effects of Secreted Factors from Periodontal Ligament Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and CM Collection
2.2. Cell Viability
2.3. Ki67 Immunostaining and DAPI Staining
2.4. Network Formation
2.5. Measurement of Growth Factors
2.6. Statistical Analysis
3. Results
3.1. Isolation and Characterization of PDLSCs
3.2. Effect of CMs on the Cell Viability of Periodontal Tissue-Composing Cells
3.3. Effect of CM on the Apoptosis of HUVECs
3.4. Effect of CM on the Cell Proliferation of HUVECs
3.5. Effect of CM on the Network Formation of HUVECs
3.6. Angiogenic Factor in PDLSC-CM
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Iwasaki, K.; Akazawa, K.; Nagata, M.; Komaki, M.; Peng, Y.; Umeda, M.; Watabe, T.; Morita, I. Angiogenic Effects of Secreted Factors from Periodontal Ligament Stem Cells. Dent. J. 2021, 9, 9. https://doi.org/10.3390/dj9010009
Iwasaki K, Akazawa K, Nagata M, Komaki M, Peng Y, Umeda M, Watabe T, Morita I. Angiogenic Effects of Secreted Factors from Periodontal Ligament Stem Cells. Dentistry Journal. 2021; 9(1):9. https://doi.org/10.3390/dj9010009
Chicago/Turabian StyleIwasaki, Kengo, Keiko Akazawa, Mizuki Nagata, Motohiro Komaki, Yihao Peng, Makoto Umeda, Tetsuro Watabe, and Ikuo Morita. 2021. "Angiogenic Effects of Secreted Factors from Periodontal Ligament Stem Cells" Dentistry Journal 9, no. 1: 9. https://doi.org/10.3390/dj9010009