Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Glutenin Characterisation and Haplotype Analysis
2.3. KASP Markers
2.4. Field Experiments
2.5. Phenotypic Evaluations
2.6. Statistical Analyses
3. Results
3.1. High-Molecular-Weight Glutenin Subunits (HMW-GS)
3.2. Low-Molecular-Weight Glutenin Subunits (LMW-GS)
3.3. Effect of HMW-GS and LMW-GS on Gluten Strength and Protein Content
3.4. HMW-GS and LMW-GS Distribution along Different Periods and Origins
3.5. Quality Traits Variation over Different Breeding Periods
3.6. Glutenin Haplotype Frequency, Distribution and Effect
3.7. Haplotype Effect on Additional Quality Parameters in Argentinian Genotypes
4. Discussion
4.1. Glutenin Allelic and Haplotype Variation and Its Effect on GPC and the SDSS Test
4.2. Variability in Quality Traits between Breeding Periods and Its Relationship with Allelic Variation at Glutenin Loci
4.3. Haplotype Effect on Additional Quality Parameters
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Argentina (85) | Chile (26) | CIMMYT (10) | France (21) | Italy (33) | USA (4) | WANA (17) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Locus/Banding Pattern | Allele | N | % | N | % | N | % | N | % | N | % | N | % | N | % | N | % |
HMW glutenin subunits | |||||||||||||||||
Glu-A1 (2) | |||||||||||||||||
2 * | b | 1 | 0.5 | 1 | 1.2 | ||||||||||||
null | c | 195 | 99.5 | 84 | 98.8 | 26 | 100.0 | 10 | 100.0 | 21 | 100.0 | 33 | 100.0 | 4 | 100.0 | 17 | 100.0 |
Glu-B1 (10) | |||||||||||||||||
7 + 8 | b | 74 | 37.8 | 28 | 32.9 | 18 | 69.2 | 5 | 50.0 | 4 | 19.0 | 12 | 39.4 | 1 | 25.0 | 6 | 35.3 |
6 + 8 | d | 52 | 26.5 | 35 | 41.2 | 6 | 23.1 | 1 | 10.0 | 4 | 19.0 | 1 | 3.0 | 3 | 75.0 | 2 | 11.8 |
20x + 20y | e | 50 | 25.5 | 16 | 18.8 | 2 | 7.7 | 2 | 20.0 | 10 | 47.6 | 12 | 33.3 | 8 | 47.1 | ||
13 + 16 | f | 5 | 2.6 | 1 | 1.2 | 1 | 4.8 | 3 | 9.1 | ||||||||
7 + 15 | z | 7 | 3.6 | 3 | 3.5 | 2 | 20.0 | 2 | 9.5 | ||||||||
6 | an | 1 | 0.5 | 1 | 1.2 | ||||||||||||
7 + 22 | ch | 4 | 2.0 | 1 | 1.2 | 3 | 9.1 | ||||||||||
14 + 22 * | new (cr) | 1 | 0.5 | 1 | 3.0 | ||||||||||||
6 * + 15 * | new (cs) | 1 | 0.5 | 1 | 5.9 | ||||||||||||
6 + 20y | new (ct) | 1 | 0.5 | 1 | 3.0 | ||||||||||||
LMW glutenin subunits | |||||||||||||||||
Glu-A3 (9) | |||||||||||||||||
6 | a | 113 | 57.7 | 50 | 58.8 | 17 | 65.4 | 8 | 80.0 | 7 | 33.3 | 25 | 75.8 | 6 | 35.3 | ||
5 | b | 7 | 3.6 | 1 | 3.8 | 2 | 6.1 | 1 | 25.0 | 3 | 17.6 | ||||||
6 + 10 | c | 52 | 26.5 | 23 | 27.1 | 7 | 26.9 | 2 | 20.0 | 9 | 42.9 | 1 | 3.0 | 2 | 50.0 | 8 | 47.1 |
6 + 11 | d | 4 | 2.0 | 1 | 1.2 | 1 | 3.8 | 1 | 4.8 | 1 | 3.0 | ||||||
11 | e | 3 | 1.5 | 2 | 9.5 | 1 | 3.0 | ||||||||||
null | h | 12 | 6.1 | 8 | 9.4 | 1 | 4.8 | 3 | 9.1 | ||||||||
6.1 + 10 | ax | 3 | 1.5 | 3 | 3.5 | ||||||||||||
5 + 10 | 1 | 0.5 | 1 | 25.0 | |||||||||||||
10 + 11 | 1 | 0.5 | 1 | 4.8 | |||||||||||||
Glu-B3 (8) | |||||||||||||||||
2 + 4 + 15 + 19 | a | 176 | 89.8 | 80 | 94.1 | 24 | 92.3 | 10 | 100.0 | 19 | 90.5 | 29 | 87.9 | 2 | 50.0 | 12 | 70.6 |
8 + 9 + 13 + 16 | b | 8 | 4.1 | 1 | 3.8 | 2 | 6.1 | 2 | 50.0 | 3 | 17.6 | ||||||
2 + 4 + 15 + 16 | g | 4 | 2.0 | 1 | 1.2 | 1 | 3.0 | 2 | 11.8 | ||||||||
1 + 3 + 14 + 18 | h | 1 | 0.5 | 1 | 3.0 | ||||||||||||
2 + 4 + 15 + 18 | ax | 2 | 1.0 | 2 | 9.5 | ||||||||||||
2 + 4 + 16 | ag | 1 | 0.5 | 1 | 1.2 | ||||||||||||
2 + 4 + 8 + 9 + 15 + 19 | new (aw) | 3 | 1.5 | 3 | 3.5 | ||||||||||||
Null | j | 1 | 0.5 | 1 | 3.8 | ||||||||||||
Glu-B2 (3) | |||||||||||||||||
12 | a | 183 | 93.4 | 81 | 95.3 | 25 | 96.2 | 10 | 100.0 | 20 | 95.2 | 29 | 87.9 | 2 | 50.0 | 16 | 94.1 |
null | b | 11 | 5.6 | 4 | 4.7 | 1 | 3.8 | 1 | 4.8 | 3 | 9.1 | 2 | 50.0 | ||||
12 * | c | 2 | 1.0 | 1 | 3.0 | 1 | 5.9 | ||||||||||
LMW-GS Model | |||||||||||||||||
1 | 8 | 4.1 | 1 | 3.8 | 2 | 6.1 | 2 | 50.0 | 3 | 17.6 | |||||||
2 | 187 | 95.4 | 85 | 100.0 | 24 | 92.3 | 10 | 100.0 | 21 | 100.0 | 31 | 93.9 | 2 | 50.0 | 14 | 82.4 | |
none | 1 | 0.5 | 1 | 3.8 | |||||||||||||
196 | 100 | 85 | 100.0 | 26 | 100.0 | 10 | 100.0 | 21 | 100.0 | 33 | 100.0 | 4 | 100.0 | 17 | 100.0 |
Loci | ||||||||
---|---|---|---|---|---|---|---|---|
Haplotype | Glu-A1 | Glu-B3 | Glu-B2 | Glu-B1 | Glu-A3 | N | Frequency (%) | Quality Traits Assessed 1 |
Hap_1 | Ax2 * | 2 + 4 + 15 + 19 | 12 | 6 + 8 | 6 | 1 | 0.5 | yes |
Hap_2 | null | 1 + 3 + 14 + 18 | 12 * | 14 + 22 * | null | 1 | 0.5 | yes |
Hap_3 | null | 2 + 4 + 15 + 16 | 12 | 20x + 20y | 6 + 10 | 1 | 0.5 | yes |
Hap_4 | null | 2 + 4 + 15 + 16 | 12 | 6 * + 15 * | 6 | 1 | 0.5 | yes |
Hap_5 | null | 2 + 4 + 15 + 16 | 12 | 20x + 20y | 6 | 1 | 0.5 | yes |
Hap_6 | null | 2 + 4 + 15 + 16 | null | 6 + 8 | 6 | 1 | 0.5 | no |
Hap_7 | null | 2 + 4 + 15 + 18 | 12 | 20x + 20y | 11 | 1 | 0.5 | yes |
Hap_8 | null | 2 + 4 + 15 + 18 | 12 | 7 + 15 | null | 1 | 0.5 | yes |
Hap_9 | null | 2 + 4 + 15 + 19 | 12 | 13 + 16 | 6 | 2 | 1.0 | yes |
Hap_10 | null | 2 + 4 + 15 + 19 | 12 | 13 + 16 | 6 + 10 | 1 | 0.5 | yes |
Hap_11 | null | 2 + 4 + 15 + 19 | 12 | 20x + 20y | 6 | 25 | 12.8 | yes |
Hap_12 | null | 2 + 4 + 15 + 19 | 12 | 20x + 20y | 6.1 + 10 | 3 | 1.5 | yes |
Hap_13 | null | 2 + 4 + 15 + 19 | 12 | 20x + 20y | 6 + 10 | 11 | 5.6 | yes |
Hap_14 | null | 2 + 4 + 15 + 19 | 12 | 20x + 20y | null | 3 | 1.5 | yes |
Hap_15 | null | 2 + 4 + 15 + 19 | 12 | 6 + 20y | 6 | 1 | 0.5 | no |
Hap_16 | null | 2 + 4 + 15 + 19 | 12 | 6 + 8 | 6 | 25 | 12.8 | yes |
Hap_17 | null | 2 + 4 + 15 + 19 | 12 | 6 + 8 | 6 + 10 | 20 | 10.2 | yes |
Hap_18 | null | 2 + 4 + 15 + 19 | 12 | 7 + 15 | 11 | 1 | 0.5 | yes |
Hap_19 | null | 2 + 4 + 15 + 19 | 12 | 7 + 15 | 6 | 5 | 2.6 | yes |
Hap_20 | null | 2 + 4 + 15 + 19 | 12 | 7 + 22 | 6 | 2 | 1.0 | yes |
Hap_21 | null | 2 + 4 + 15 + 19 | 12 | 7 + 22 | null | 2 | 1.0 | yes |
Hap_22 | null | 2 + 4 + 15 + 19 | 12 | 7 + 8 | 6 | 44 | 22.4 | yes |
Hap_23 | null | 2 + 4 + 15 + 19 | 12 | 7 + 8 | 6 + 10 | 18 | 9.2 | yes |
Hap_24 | null | 2 + 4 + 15 + 19 | 12 | 7 + 8 | 6 + 11 | 4 | 2.0 | yes |
Hap_25 | null | 2 + 4 + 15 + 19 | 12 | 7 + 8 | null | 2 | 1.0 | no |
Hap_26 | null | 2 + 4 + 15 + 19 | null | 13 + 16 | 11 | 1 | 0.5 | yes |
Hap_27 | null | 2 + 4 + 15 + 19 | null | 20x + 20y | 6 | 1 | 0.5 | yes |
Hap_28 | null | 2 + 4 + 15 + 19 | null | 20x + 20y | 6 + 10 | 1 | 0.5 | yes |
Hap_29 | null | 2 + 4 + 15 + 19 | null | 20x + 20y | null | 1 | 0.5 | yes |
Hap_30 | null | 2 + 4 + 15 + 19 | null | 6 + 8 | 6 | 1 | 0.5 | no |
Hap_31 | null | 2 + 4 + 15 + 19 | null | 7 + 8 | 10 + 11 | 1 | 0.5 | yes |
Hap_32 | null | 2 + 4 + 16 | 12 | 13 + 16 | 6 | 1 | 0.5 | no |
Hap_33 | null | 2 + 4 + 8 + 9 + 15 + 19 | 12 | 6 | 6 | 1 | 0.5 | no |
Hap_34 | null | 2 + 4 + 8 + 9 + 15 + 19 | 12 | 6 + 8 | null | 2 | 1.0 | no |
Hap_35 | null | 8 + 9 + 13 + 16 | 12 | 20x + 20y | 5 | 1 | 0.5 | yes |
Hap_36 | null | 8 + 9 + 13 + 16 | 12 | 7 + 8 | 5 | 3 | 1.5 | yes |
Hap_37 | null | 8 + 9 + 13 + 16 | 12 * | 7 + 8 | 5 | 1 | 0.5 | yes |
Hap_38 | null | 8 + 9 + 13 + 16 | null | 20x + 20y | 5 | 1 | 0.5 | yes |
Hap_39 | null | 8 + 9 + 13 + 16 | null | 6 + 8 | 5 | 1 | 0.5 | yes |
Hap_40 | null | 8 + 9 + 13 + 16 | null | 6 + 8 | 5 + 10 | 1 | 0.5 | yes |
Hap_41 | null | null | null | 7 + 8 | 6 | 1 | 0.5 | yes |
Total (N or %) | 2 | 8 | 3 | 10 | 9 | 196 | 100.0 |
2011 | GPC 1 | SDSS Test | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Locus/Banding Pattern | Allele | N | % | CA 2011 | BW 2011 | PS 2011 | LSmean | CA 2011 | BW 2011 | PS 2011 | LSmean |
HMW glutenin subunits | |||||||||||
Glu-A1 (2) | |||||||||||
2 * | b | 1 | 0.8 | 13.92 a | 12.29 a | 11.5 a | 12.58 a | 84.50 a | 86.66 a | 68.51 a | 80.17 a |
null | c | 131 | 99.2 | 14.62 a | 13.39 a | 11.95 a | 13.32 a | 62.11 a | 66.51 a | 54.16 a | 60.92 a |
Glu-B1 (10) | |||||||||||
7 + 8 | b | 42 | 31.8 | 14.49 a | 13.28 a | 11.93 a | 13.23 a | 67.6 b | 71.18 b | 61.94 b | 66.34 b |
6 + 8 | d | 33 | 25.0 | 14.81 a | 13.32 ab | 11.85 a | 13.32 a | 71.97 b | 77.19 b | 62.58 b | 70.63 b |
20x + 20y | e | 43 | 32.6 | 14.59 a | 13.43 ab | 11.96 a | 13.33 a | 48.69 ab | 52.66 ab | 38.87 ab | 46.71 ab |
13 + 16 | f | 4 | 3.0 | 14.48 a | 13.45 ab | 11.78 a | 13.23 a | 61.0 b | 74.37 b | 54.24 ab | 63.34 b |
7 + 15 | z | 4 | 3.0 | 14.57 a | 13.49 ab | 12.15 a | 13.41 a | 73.13 b | 77.85 b | 69.14 b | 73.38 b |
6 | an | na | na | na | na | na | na | na | na | na | na |
7 + 22 | ch | 4 | 3.0 | 14.54 a | 13.84 ab | 12.35 a | 13.59 a | 69.0 b | 71.62 b | 60.43 b | 66.92 b |
14 + 22 * | new (cr) | 1 | 0.8 | 14.99 a | 14.78 b | 13.18 a | 14.3 a | 24.5 a | 19.61 a | 20.6 a | 21.83 a |
6 * + 15 * | new (cs) | 1 | 0.8 | 14.99 a | 14.17 ab | 12.47 a | 13.9 a | 80.5 b | 88.57 b | 70.82 b | 79.83 b |
6 + 20y | new (ct) | na | na | na | na | na | na | na | na | na | na |
LMW glutenin subunits | |||||||||||
Glu-A3 (9) | |||||||||||
6 | a | 74 | 56.1 | 14.65 a | 13.41 ab | 11.99 a | 13.35 a | 64.72 b | 68.53 b | 56.55 ab | 63.28 b |
5 | b | 6 | 4.5 | 14.84 a | 13.85 ab | 12.35 a | 13.67 a | 22.0 a | 22.36 a | 18.25 a | 20.94 a |
6 + 10 | c | 37 | 28.0 | 14.46 a | 13.21 ab | 11.8 a | 13.15 a | 64.31 b | 70.92 b | 56.27 ab | 63.83 b |
6 + 11 | d | 1 | 0.8 | 15.09 a | 13.25 ab | 11.94 a | 13.4 a | 56.5 ab | 75.98 b | 52.84 ab | 61.67 b |
11 | e | 3 | 2.3 | 14.6 a | 13.23 ab | 11.88 a | 13.24 a | 69.8 b | 71.37 b | 52.37 ab | 64.61 b |
null | h | 7 | 5.3 | 14.94 a | 14.14 b | 12.4 a | 13.84 a | 57.0 ab | 60.11 ab | 48.49 ab | 55.0 ab |
6.1 + 10 | ax | 3 | 2.3 | 14.09 a | 12.64 a | 11.0 a | 12.57 a | 59.2 ab | 57.3 ab | 54.02 ab | 56.83 ab |
5 + 10 | na | na | na | na | na | na | na | na | na | na | |
10 + 11 | 1 | 0.8 | 14.34 a | 13.01 ab | 11.9 a | 13.12 a | 78.0 b | 86.87 b | 75.79 b | 80.17 b | |
Glu-B3 (8) | |||||||||||
2 + 4 + 15 + 19 | a | 120 | 90.9 | 14.59 a | 13.34 a | 11.9 a | 13.28 a | 64.9 c | 69.6 b | 56.79 a | 63.78 b |
8 + 9 + 13 + 16 | b | 6 | 4.5 | 14.84 a | 13.85 ab | 12.35 a | 13.67 a | 22.0 a | 22.36 a | 18.25 a | 20.94 a |
2 + 4 + 15 + 16 | g | 3 | 2.3 | 14.41 a | 13.69 ab | 12.47 a | 13.53 a | 51.8 abc | 52.75 ab | 45.42 a | 49.78 ab |
1 + 3 + 14 + 18 | h | 1 | 0.8 | 14.99 a | 14.78 b | 13.18 a | 14.3 a | 24.5 ab | 15.5 a | 20.6 a | 21.83 a |
2 + 4 + 15 + 18 | ax | 2 | 1.5 | 15.34 a | 13.54 a | 12.35 a | 13.75 a | 58.3 bc | 67.07 b | 41.03 a | 55.25 ab |
2 + 4 + 16 | ag | na | na | na | na | na | na | na | na | na | na |
2 + 4 + 8 + 9 + 15 + 19 | new (aw) | na | na | na | na | na | na | na | na | na | na |
Null | j | na | na | na | na | na | na | na | na | na | na |
Glu-B2 (3) | |||||||||||
12 | a | 125 | 94.7 | 14.60 a | 13.37 a | 11.92 a | 13.3 a | 63.76 b | 68.35 b | 55.48 b | 62.52 b |
null | b | 5 | 3.8 | 14.86 a | 13.51 a | 12.23 a | 13.55 a | 42.8 ab | 44.22 ab | 39.5 ab | 42.23 ab |
12 * | c | 2 | 1.5 | 14.47 a | 14.08 a | 12.84 a | 13.78 a | 18.75 a | 16.93 a | 15.59 a | 17.17 b |
LMW-GS Model | |||||||||||
1 | 6 | 4.5 | 14.6 a | 13.85 b | 12.35 a | 13.67 a | 22.0 a | 22.4 a | 18.3 a | 20.9 a | |
2 | 126 | 95.5 | 14.84 a | 13.36 a | 11.93 a | 13.3 a | 64.2 b | 68.8 b | 56.0 b | 60.98 b | |
none | na | na | na | na | na | na | na | na | na | na |
2014/17 | GPC 1 | SDSS Test | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Locus/Banding Pattern | Allele | N | % | CA 2014 | BW 2014 | PS 2014 | BW 2017 | LSmean | CA 2014 | BW 2014 | PS 2014 | LSmean |
HMW glutenin subunits | ||||||||||||
Glu-A1 (2) | ||||||||||||
2 * | b | 1 | 0.6 | 13.2 a | 10.61 a | 11.54 a | 10.51 a | 11.64 a | 72.08 a | 68.99 a | 59.08 a | 66.84 a |
null | c | 161 | 94.7 | 12.98 a | 12.2 a | 12.37 a | 11.14 a | 12.16 a | 58.81 a | 66.38 a | 66.28 a | 63.83 a |
Glu-B1 (10) | ||||||||||||
7 + 8 | b | 65 | 38.2 | 12.49 ab | 12.31 a | 12.31 a | 10.93 a | 12.0 a | 61.99 b | 73.03 b | 71.16 b | 68.69 b |
6 + 8 | d | 44 | 25.9 | 13.68 ab | 11.94 a | 12.34 a | 11.23 ab | 12.29 a | 64.82 b | 66.39 ab | 67.48 b | 66.31 b |
20x + 20y | e | 47 | 27.6 | 13.08 ab | 12.26 a | 12.41 a | 11.3 ab | 12.27 a | 48.12 ab | 55.19 ab | 56.46 ab | 53.27 ab |
13 + 16 | f | 4 | 2.4 | 11.73 ab | 11.6 a | 11.98 a | 11.23 ab | 11.59 a | 59.34 ab | 77.87 b | 78.02 b | 71.61 b |
7 + 15 | z | 4 | 2.4 | 12.32 ab | 11.81 a | 12.42 a | 10.94 a | 11.9 a | 61.59 b | 69.14 b | 78.82 b | 69.63 b |
6 | an | na | na | |||||||||
7 + 22 | ch | 4 | 2.4 | 14.04 ab | 12.58 a | 12.33 a | 11.26 ab | 12.48 ab | 74.9 b | 80.61 b | 72.01 b | 76.15 b |
14 + 22 * | new (cr) | 1 | 0.6 | 15.38 b | 12.5 a | 14.82 b | 12.7 b | 14.02 b | 18.41 a | 21.97 a | 21.28 a | 20.31 a |
6 * + 15 * | new (cs) | 1 | 0.6 | 11.43 a | 13.31 a | 13.67 b | 11.82 ab | 12.35 a | 68.67 b | 87.02 b | 72.0 b | 75.48 b |
6 + 20y | new (ct) | na | na | |||||||||
LMW glutenin subunits | ||||||||||||
Glu-A3 (9) | ||||||||||||
6 | a | 98 | 57.6 | 12.98 a | 12.23 a | 12.39 ab | 11.11 ab | 12.07 a | 61.92 abc | 69.18 bc | 70.11 b | 67.10 c |
5 | b | 6 | 3.5 | 13.06 a | 13.12 a | 13.05 ab | 11.69 ab | 12.77 a | 18.72 a | 19.9 a | 23.6 a | 20.66 a |
6 + 10 | c | 48 | 28.2 | 12.89 a | 12.08 a | 12.23 ab | 11.08 ab | 12.07 a | 58.11 abc | 68.68 bc | 64.69 ab | 63.81 bc |
6 + 11 | d | 2 | 1.2 | 13.80 a | 11.97 a | 12.44 ab | 10.73 ab | 12.24 a | 67.58 bc | 73.09 c | 60.76 ab | 67.15 c |
11 | e | 3 | 1.8 | 11.92 a | 11.77 a | 12.03 ab | 11.42 ab | 11.78 a | 61.25 abc | 67.0 abc | 72.51 b | 66.55 c |
null | h | 8 | 4.7 | 13.35 a | 12.15 a | 12.65 ab | 11.44 ab | 12.43 a | 57.36 abc | 64.48 abc | 64.27 ab | 62.03 bc |
6.1 + 10 | ax | 3 | 1.8 | 13.86 a | 11.29 a | 11.15 a | 10.8 ab | 11.72 a | 51.01 abc | 42.33 abc | 58.57 ab | 50.7 abc |
5 + 10 | 1 | 0.6 | 14.24 a | 11.99 a | 11.96 ab | 12.3 ab | 12.64 a | 31.45 ab | 21.96 ab | 23.78 a | 25.97 ab | |
10 + 11 | 1 | 0.6 | 11.79 a | 12.33 a | 13.29 b | 10.42 a | 11.69 a | 78.4 c | 80.56 c | 90.74 b | 83.22 c | |
Glu-B3 (8) | ||||||||||||
2 + 4 + 15 + 19 | a | 156 | 117.3 | 12.99 a | 12.15 a | 12.32 a | 11.10 ab | 12.13 a | 61.17 ab | 68.92 bc | 68.42 b | 66.18 b |
8 + 9 + 13 + 16 | b | 7 | 5.3 | 13.23 a | 12.96 a | 12.98 ab | 11.78 ab | 12.75 ab | 20.54 a | 20.20 a | 23.63 a | 21.42 a |
2 + 4 + 15 + 16 | g | 3 | 2.3 | 11.96 a | 12.32 a | 12.26 a | 11.32 ab | 11.95 a | 49 ab | 67.52 abc | 58.82 ab | 58.59 ab |
1 + 3 + 14 + 18 | h | 1 | 0.8 | 15.38 b | 12.5 a | 14.82 b | 12.70 b | 14.02 b | 18.41 a | 21.97 ab | 21.88 a | 20.31 a |
2 + 4 + 15 + 18 | ax | 2 | 1.5 | 10.92 a | 12.54 a | 12.81 ab | 10.88 ab | 11.96 a | 45.83 ab | 44.0 abc | 70.91 b | 53.48 ab |
2 + 4 + 16 | ag | na | na | |||||||||
2 + 4 + 8 + 9 + 15 + 19 | new (aw) | na | na | |||||||||
Null | j | 1 | 0.8 | 14.61 ab | 10.97 a | 12.11 a | 10.60 a | 12.06 a | 66.5 b | 82.04 c | 81.69 b | 76.94 b |
Glu-B2 (3) | ||||||||||||
12 | a | 159 | 93.5 | 12.96 a | 12.19 a | 12.33 a | 11.11 a | 12.14 a | 60.06 b | 68.02 b | 67.54 b | 65.22 b |
null | b | 9 | 5.3 | 13.16 a | 11.96 a | 12.44 a | 11.36 a | 12.25 a | 48.00 b | 48.89 b | 54.30 b | 50.31 b |
12 * | c | 2 | 1.2 | 14.08 a | 12.88 a | 14.48 b | 12.07 a | 13.43 b | 14.65 a | 16.43 a | 16.66 a | 15.52 a |
LMW-GS Model | ||||||||||||
1 | 7 | 4.1 | 13.23 a | 12.96 b | 12.98 a | 11.78 a | 12.74 a | 20.54 a | 20.20 a | 23.63 a | 21.42 a | |
2 | 162 | 95.3 | 12.96 a | 12.16 ab | 12.34 a | 11.11 a | 12.14 a | 60.5 b | 68.3 b | 67.98 b | 65.6 b | |
none | 1 | 0.588 | 14.61 a | 10.88 a | 12.11 a | 10.60 a | 12.06 a | 66.5 b | 82.04 b | 81.68 b | 76.94 b |
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Roncallo, P.F.; Guzmán, C.; Larsen, A.O.; Achilli, A.L.; Dreisigacker, S.; Molfese, E.; Astiz, V.; Echenique, V. Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes. Foods 2021, 10, 2845. https://doi.org/10.3390/foods10112845
Roncallo PF, Guzmán C, Larsen AO, Achilli AL, Dreisigacker S, Molfese E, Astiz V, Echenique V. Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes. Foods. 2021; 10(11):2845. https://doi.org/10.3390/foods10112845
Chicago/Turabian StyleRoncallo, Pablo F., Carlos Guzmán, Adelina O. Larsen, Ana L. Achilli, Susanne Dreisigacker, Elena Molfese, Valentina Astiz, and Viviana Echenique. 2021. "Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes" Foods 10, no. 11: 2845. https://doi.org/10.3390/foods10112845
APA StyleRoncallo, P. F., Guzmán, C., Larsen, A. O., Achilli, A. L., Dreisigacker, S., Molfese, E., Astiz, V., & Echenique, V. (2021). Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes. Foods, 10(11), 2845. https://doi.org/10.3390/foods10112845