Impact of Hydrolyzed Collagen from Defatted Sea Bass Skin on Proliferation and Differentiation of Preosteoblast MC3T3-E1 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Enzyme Assays
2.3. Preparation and Pretreatment of Sea Bass Skin Using Pulsed Electric Field (PEF)
2.4. Defatting of PEF-Treated Skin
2.5. HC Preparation
2.6. Size Distribution
2.7. Impact of HC on Proliferation and Differentiation of MC3T3-E1 Preosteoblast Cells
2.7.1. Cell Culture
2.7.2. Cell Proliferation Assays
2.7.3. Alkaline Phosphatase Activity (AP-A)
2.7.4. Determination of Mineralization in MC3T3-E1 Cells
2.7.5. Western Blot Analysis
2.7.6. Effect of HC Combined with Inhibitor on AP-A and Mineralization in Cells
2.8. Statistical Analysis
3. Results
3.1. Size Distribution of HC
3.2. Impact of HC on Proliferation, Alkaline Phosphatase Activity and Mineralization of MC3T3-E1 Cells
3.2.1. MC3T3-E1 Cell Proliferation
3.2.2. Alkaline Phosphatase Activity (AP-A)
3.2.3. Mineralization of MC3T3-E1 Cells
3.3. Effect of HC on RUNX2 Expression in MC3T3-E1 Cells
3.4. Effect of HC on Akt Signaling Pathway in MC3T3-E1 Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chotphruethipong, L.; Binlateh, T.; Hutamekalin, P.; Aluko, R.E.; Tepaamorndech, S.; Zhang, B.; Benjakul, S. Impact of Hydrolyzed Collagen from Defatted Sea Bass Skin on Proliferation and Differentiation of Preosteoblast MC3T3-E1 Cells. Foods 2021, 10, 1476. https://doi.org/10.3390/foods10071476
Chotphruethipong L, Binlateh T, Hutamekalin P, Aluko RE, Tepaamorndech S, Zhang B, Benjakul S. Impact of Hydrolyzed Collagen from Defatted Sea Bass Skin on Proliferation and Differentiation of Preosteoblast MC3T3-E1 Cells. Foods. 2021; 10(7):1476. https://doi.org/10.3390/foods10071476
Chicago/Turabian StyleChotphruethipong, Lalita, Thunwa Binlateh, Pilaiwanwadee Hutamekalin, Rotimi E. Aluko, Surapun Tepaamorndech, Bin Zhang, and Soottawat Benjakul. 2021. "Impact of Hydrolyzed Collagen from Defatted Sea Bass Skin on Proliferation and Differentiation of Preosteoblast MC3T3-E1 Cells" Foods 10, no. 7: 1476. https://doi.org/10.3390/foods10071476