Study and Experimental Validation of the Functional Components and Mechanisms of Hemerocallis citrina Baroni in the Treatment of Lactation Deficiency
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Preparation of HCE Extracts
2.3. Chromatography and MS
2.4. Experiments Performed with Animals
2.5. Stress Procedure
2.6. Sample Collection
2.7. Evaluation of Lactation Promoting Activity
2.7.1. Weight Changes of Pups
2.7.2. Biochemical Analyses
2.7.3. Histopathological Analysis
2.8. Analysis of Potential Mechanism
2.8.1. Screening Targets of HCE–Lactation
2.8.2. PPI Network Construction
2.8.3. KEGG Enrichment Analysis
2.9. Total RNA Extraction and Real Time PCR
2.10. Statistical Analysis
3. Results
3.1. Identification of the Components in HCE Based on UPLC-Q-Orbitrap-MS and OTCML
3.2. Effects of HCE on Lactation of CUMS Dams
3.2.1. Pups Weight Change
3.2.2. Serum PRL and OT Level
3.2.3. Mammary Gland Tissue of Dams
3.3. Analysis and Validation of Potential Therapeutic Mechanism of HCE
3.3.1. Potential Target Screening of HCE by Network Pharmacology
3.3.2. Construction of “HCE-Targets-Lactation” Network
3.3.3. PPI Network of HCE-Lactation
3.3.4. KEGG Enrichment Analysis
3.3.5. Gene Expression of Prolactin Signaling Pathway
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No. | Name | Degree | Betweenness Centrality | Closeness Centrality |
---|---|---|---|---|
HCE61 | Quercetin | 95 | 0.09 | 0.49 |
HCE55 | Kaempferol | 52 | 0.02 | 0.42 |
HCE25 | Thymidine | 47 | 0.02 | 0.42 |
HCE28 | Chlorogenic acid | 42 | 0.01 | 0.41 |
HCE32 | Caffeic acid | 41 | 0.01 | 0.41 |
HCE34 | Rutin | 40 | 0.01 | 0.41 |
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Zhong, J.; Liang, Y.; Chen, Y.; Zhang, J.; Zou, X.; Deng, J.; Wang, D.; Sun, Y.; Li, M. Study and Experimental Validation of the Functional Components and Mechanisms of Hemerocallis citrina Baroni in the Treatment of Lactation Deficiency. Foods 2021, 10, 1863. https://doi.org/10.3390/foods10081863
Zhong J, Liang Y, Chen Y, Zhang J, Zou X, Deng J, Wang D, Sun Y, Li M. Study and Experimental Validation of the Functional Components and Mechanisms of Hemerocallis citrina Baroni in the Treatment of Lactation Deficiency. Foods. 2021; 10(8):1863. https://doi.org/10.3390/foods10081863
Chicago/Turabian StyleZhong, Jing, Yuxuan Liang, Yongchun Chen, Jiawei Zhang, Xiaoying Zou, Jie Deng, Da Wang, Yuanming Sun, and Meiying Li. 2021. "Study and Experimental Validation of the Functional Components and Mechanisms of Hemerocallis citrina Baroni in the Treatment of Lactation Deficiency" Foods 10, no. 8: 1863. https://doi.org/10.3390/foods10081863