Dynamic Changes in Vitamin E Biosynthesis during Germination in Brown Rice (Oryza sativa L.)
, Yingxin Lin 2, Jiayan Liang 1, Xiaodan Hu 1, Umair Ashraf 3, Xinbo Guo 2,* and Song Bai 1,*Round 1
Reviewer 1 Report
The authors studied dynamic changes of vitamin E content during brown rice germination and correlated it to the expression of key genes of vitamin E biosynthesis.
The topic of the present manuscript is interesting and cover a good scope, relevant to the Journal. The manuscript is well organized and written. The data reported are relevant and well discussed.
some minor modifications and comments:
- - Line 57: please delete “Figure 1”
- - Materials and methods section: did the standards used for HPLC vitamin E determination includes all the homologues? Please clarify.
- - Line 112: in figure 2 are reported changes of vitamin E during germination, not morphological data. Please, correct.
- - In the figure of Heat map of dynamic changes of vitamin E some data are lacking (the color of some bands for b-tocopherol is lacking)
- - Line 125: please clarify how did you identify and quantify the vitamins homologues.
- - Line 151: Please check and correct the number of all the figures of paragraphs 3.1 and 3.2.
- - Line 240: I think is better to use the term “germination” instead of “generation”.
- - Line 242: please change “that” with “where”.
- - Line 251: GRB is GBR?
- - Lines 251-252: the sentences are not clear. Please, check and correct.
- - Bibliography: please check and correct the citation format.
Author Response
Please see the attachment.
Author Response File: 
Author Response.docx
Reviewer 2 Report
This manuscript entitled “Dynamic changes in biosynthesis of vitamin E during germination in brown rice” is an interesting and original study.
The paper is clearly presented and results are very useful. However, I have some suggestions:
1. Review the figure captions of the manuscript. The figures must be independent and understandable by themselves. Indicate what each code is in the figure caption.
2. Figure 3 is not related to correlations. Why does it indicate in its caption some correlations?
Author Response
Please see the attachment.
Author Response File: Author Response.docx
