Effect of Combined Treatment with Cinnamon Oil and petit-High Pressure CO2 against Saccharomyces cerevisiae
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Yeast Strain
2.2. The Minimum Inhibitory Concentration of CIN
2.3. Treatment with CIN Combined with p-HPCO2
2.4. Synergism Assessment
2.5. Field Emission-Scanning Electron Microscopy
2.6. Leakage of Intracellular DNA
2.7. Cellular Membrane Integrity
2.8. Cellular Membrane Potential
2.9. Expression of the Genes Related to Ergosterol Biosynthesis
2.10. Mitochondrial Membrane Potential
2.11. Intracellular ATP Content
2.12. Statistical Analysis
3. Results and Discussion
3.1. The MIC of CIN
3.2. Antifungal Analysis of CIN + p-HPCO2
3.3. Cell Morphological Characterization
3.4. Cell Membrane Permeability
3.5. Plasma Membrane Integrity
3.6. Cell Membrane Potential
3.7. Expression of Genes Related to Ergosterol Biosynthesis
3.8. Mitochondrial Membrane Potential
3.9. Intracellular ATP
3.10. Synergistic Antifungal Mechanism of CIN + p-HPCO2
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer Sequences (5′-3′) | Amplicon Size (bp) | |
---|---|---|---|
ACT1 | F | ACCGCTGCTCAATCTTCTTC | 164 |
R | ATGATGGAGTTGTAAGTAGTTTGG | ||
HMG2 | F | GGTTGGGAAGATATGGAAGTTG | 131 |
R | ACGACATCACCAGGAATAGTAG | ||
ERG6 | F | AAGACCTGGCGGACAATGATG | 199 |
R | AGAGCAGCAGTAACTTCCTTGG | ||
ERG8 | F | AGTGGCTTCATTCCTGTTTCG | 177 |
R | TTCGGTAACGCTATCCTCCTG | ||
ERG10 | F | TCCGCTATGAAGGCAATC | 163 |
R | CGACACCATCAACAAGAAC |
Observed Log Reduction | Expected Log Reduction | Ratio | Synergism | |
---|---|---|---|---|
0.005% | ||||
p-HPCO2 | 0.38 | |||
CIN | 0.40 | |||
p-HPCO2 + CIN | 1.54 | 0.78 | 1.97 | Yes |
0.01% | ||||
p-HPCO2 | 0.38 | |||
CIN | 0.54 | |||
p-HPCO2 + CIN | 2.97 | 0.92 | 3.24 | Yes |
0.02% | ||||
p-HPCO2 | 0.31 | |||
CIN | 1.11 | |||
p-HPCO2 + CIN | 3.34 | 1.42 | 2.36 | Yes |
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Niu, L.; Liu, J.; Wang, X.; Wu, Z.; Xiang, Q.; Bai, Y. Effect of Combined Treatment with Cinnamon Oil and petit-High Pressure CO2 against Saccharomyces cerevisiae. Foods 2022, 11, 3474. https://doi.org/10.3390/foods11213474
Niu L, Liu J, Wang X, Wu Z, Xiang Q, Bai Y. Effect of Combined Treatment with Cinnamon Oil and petit-High Pressure CO2 against Saccharomyces cerevisiae. Foods. 2022; 11(21):3474. https://doi.org/10.3390/foods11213474
Chicago/Turabian StyleNiu, Liyuan, Jingfei Liu, Xinpei Wang, Zihao Wu, Qisen Xiang, and Yanhong Bai. 2022. "Effect of Combined Treatment with Cinnamon Oil and petit-High Pressure CO2 against Saccharomyces cerevisiae" Foods 11, no. 21: 3474. https://doi.org/10.3390/foods11213474