Curcumin-Mediated Sono-Photodynamic Treatment Inactivates Listeria monocytogenes via ROS-Induced Physical Disruption and Oxidative Damage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Bacterial Suspension and Curcumin Solution
2.2. Sono-Photodynamic Sterilization Treatment
2.3. Detection of Reactive Oxygen Species (ROS)
2.4. Scanning Electron Microscopy (SEM)
2.5. Determination of Cytoplasmic Material Leakage
2.6. DNA Agarose Gel Electrophoresis
2.7. Polyacrylamide Gel Electrophoresis (SDS-PAGE)
2.8. Annexin V-FITC/PI Staining Assay
2.9. Data Statistics and Analysis
3. Results
3.1. SPDT Showed Effective Bactericidal Activity against LMO
3.2. SPDT Generated Significant Intracellular ROS in LMO
3.3. SPDT Altered Morphology of LMO
3.4. SPDT Induced Cytoplasmic Material Leakage in LMO
3.5. SPDT Induced DNA Fragmentation and Protein Degradation in LMO
3.6. SPDT Induced Membrane Depolarization in LMO
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Zhang, J.; Zheng, P.; Li, J.; Yang, Y.; Zeng, S.; Qiu, J.; Lin, S. Curcumin-Mediated Sono-Photodynamic Treatment Inactivates Listeria monocytogenes via ROS-Induced Physical Disruption and Oxidative Damage. Foods 2022, 11, 808. https://doi.org/10.3390/foods11060808
Zhang J, Zheng P, Li J, Yang Y, Zeng S, Qiu J, Lin S. Curcumin-Mediated Sono-Photodynamic Treatment Inactivates Listeria monocytogenes via ROS-Induced Physical Disruption and Oxidative Damage. Foods. 2022; 11(6):808. https://doi.org/10.3390/foods11060808
Chicago/Turabian StyleZhang, Jiawen, Peiying Zheng, Jing Li, Yijing Yang, Shaoxiao Zeng, Jianqing Qiu, and Shaoling Lin. 2022. "Curcumin-Mediated Sono-Photodynamic Treatment Inactivates Listeria monocytogenes via ROS-Induced Physical Disruption and Oxidative Damage" Foods 11, no. 6: 808. https://doi.org/10.3390/foods11060808