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Volume 11
Issue 7
10.3390/foods11070988
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Article
Peer-Review Record

Ultrasound Improved the Non-Covalent Interaction of β-Lactoglobulin with Luteolin: Regulating Human Intestinal Microbiota and Conformational Epitopes Reduced Allergy Risks

Foods 2022, 11(7), 988; https://doi.org/10.3390/foods11070988
by Titi Wang 1, Wenmei Chen 1, Yanhong Shao 1, Jun Liu 1 and Zongcai Tu 1,2,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Foods 2022, 11(7), 988; https://doi.org/10.3390/foods11070988
Submission received: 28 February 2022 / Revised: 21 March 2022 / Accepted: 24 March 2022 / Published: 29 March 2022
(This article belongs to the Special Issue Impact of Pretreatment on Physicochemical and Nutritional Properties of Milk Protein)

Round 1

Reviewer 1 Report

This an interesting study of the interaction of Luteolin and beta-LG. However, the  effect of ultrasound (US) seems not to be so clear described, and I am unsure about it effects. So this needs a more trorough  described

When applying US the temperature increase in the fluid, and this need to be measured in order to understand the denaturation of the protein.

And more literature comparation with heat denaturation of Beta-lg could also improve the paper

Specific comments:

Material and methods: In general info about the type of instrument use is lacking

 

L79 This is a Low concentration of Beta-LG. I doubt whether this is food or instant formula relevant.

L81-82. What kind of ultrasonic systems is used (E.g. brand, sonotrode system,  tip size). How much did the temperature increase, this is important according to whey protein denaturation

 

L159-171: The effect of US is not clear described

Figure 4 How much here is statistically significant and is one-way ANOVA the correct analysis here. Standard deviation should also be illustrated .

L259 However histamine is not lower than control- describe and explain

L340 is this the discussion section

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

The concept of the research article is interesting. However, in the present form, it required substantial major revision. The major comments are as follows;

Introduction looks very general, in the introduction section, write the novelty of the work and the problem statement clearly. In order to the improvement of the quality content author should refer or cite recent articles related to the interaction of milk compounds. 

Section 3.1 (Infrared spectral analysis) should be rewritten as lot of grammar mistakes are there, also check in the whole manuscript.

Conclusion should be more detailed and brief.

 

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Round 2

Reviewer 2 Report

Authors included all the suggested changes in the manuscript. Now it can be considered for publication

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