Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS
, Siddabasave Gowda B. Gowda 1,2,*, Divyavani Gowda 2, Ken Inui 3 and Shu-Ping Hui 2,*
Round 1
Reviewer 1 Report
Please see the attached file and address all my comments.
Comments for author File: 
Comments.docx
Moderate editing of English language is required as well as the consistency and spacing check.
Author Response
Response letter attached.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The authors developed a Lipid analysis for Adzuki and Soybean via LC-MS. The article is interesting and well written. The novelty is well achieved. However, many revisions are required.
1- In the title correct LC/MS to be LC-MS
2- The new findings and merits for the new method over the old studies in literature should be highlighted in more clear way in the abstract.
3- The following articles should be cited in the introduction
Nutritional Composition, Efficacy, and Processing of Vigna angularis (Adzuki Bean) for the Human Diet: An Overview
https://www.mdpi.com/1420-3049/27/18/6079
Determination of phospholipids in soybean (Glycine max (L.) Merr) cultivars by liquid chromatography–tandem mass spectrometry
https://doi.org/10.1016/j.jfca.2009.12.015
lines 63-67 should be corrected in the light of the aforementioned articles
4- Figures for Adzuki and Soybean should be added for figures in the main text or in the supplementary materials
5- Reference 43 is incomplete “Liu, Y.; Chen, M.; Li, Y.; Feng, X.; Chen, Y.; Lin, L. Liquid Chromatography – Time-of-Flight Tandem Mass Spectrometry. 2022”
6- In line 76, the geographical origins for the three different cultivars should be stated.
7- Lipid extraction 2.3 needs appropriate reference.
8- In line 134, in five different cultivars of beans while in line 145, The lipid composition of all six cultivars ; correct please
9- Full names for SG 28:1; O; Hex, SG 29:1; O; Hex, and PG (18:2/18:2), should be stated.
10- Abbreviation list is strongly recommended
11- Surprisingly, no tables are present. Item 3.5 can be represented in a table form .
12- Figure 6 should be relocated to be in supplementary material to be displayed with the original size chromatograms for more readability.
13- Conclusions are interesting. However, it should be written with full names without abbreviations.
Best wishes
Author Response
Response letter attached
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
The new Figure 6 is still incorrect. Please replace [M-CH3COOH]- with [M-H]-.
Note: M generally means molecular mass. Thus, [M-CH3COOH]- means loss of CH3COOH mass from the molecular mass which is not true in the MS/MS results in Figure 6. The corrected one should be loss of CH3COOH from [M+CH3COO]- which is [M-H]-.
Author Response
Comment: The new Figure 6 is still incorrect. Please replace [M-CH3COOH]- with [M-H]-.Note: M generally means molecular mass. Thus, [M-CH3COOH]- means loss of CH3COOH mass from the molecular mass which is not true in the MS/MS results in Figure 6. The corrected one should be loss of CH3COOH from [M+CH3COO]- which is [M-H]-.
Response: Thank you for your kind suggestion again. We have replaced the [M-CH3COOH]- with [M-H]- ion in the Figure 6 as suggested. Kindly see the revised Figure 6.
Reviewer 2 Report
I appreciate their responses. The authors did all required changes.
Author Response
Comment: I appreciate their responses. The authors did all required changes.
Response: Thank you for your appreciations.
