Arctoscopus japonicus Lipids Enhance Immunity of Mice with Cyclophosphamide-Induced Immunosuppression
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Isolation of Lipids from A. japonicus Eggs
2.3. Preparation of AJ-PEG
2.4. Establishment of Immunosuppressed Mice Models
2.5. Preparation of Peritoneal Macrophages
2.6. Isolation of Spleen Lymphocytes
2.7. Determination of Peritoneal Macrophage Proliferation and Nitric Oxide (NO) Production
2.8. Measurement of Peritoneal Macrophage Phagocytosis
2.9. Proliferation of Splenic Lymphocytes
2.10. NK Cell Activity of Splenocytes
2.11. Measurement of Immune-Associated Gene Expression
2.12. Measurement of T-Lymphocyte Subsets in the Spleen
2.13. Statistical Analysis
3. Results
3.1. Fatty Acid Profiles of A. japonicus Lipids Incorporated with PEG6000 (AJ-PEG)
3.2. Effect of AJ-PEG on Spleen Index
3.3. Effect of AJ-PEG on Splenic Lymphocyte Proliferation
3.4. Effect of AJ-PEG on NK Cell Activity of Splenocytes
3.5. Effect of AJ-PEG on Gene Expression in Splenic Lymphocytes
3.6. Effect of AJ-PEG on T Lymphocytes’ Subsets of Splenocytes
3.7. Effect of AJ-PEG on Peritoneal Macrophage Proliferation and NO Production
3.8. Effect of AJ-PEG on Peritoneal Macrophage Phagocytosis
3.9. Effect of AJ-PEG on Gene Expression in Peritoneal Macrophages
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Group | Dose (mg/kg BW) | Treatment | ||
---|---|---|---|---|
Day 1 to 3 (Orally) | Day 4 to 6 (Orally + i.p.) | Day 7 to 10 (Orally) | ||
Normal | - | Saline | Saline | Saline |
CY | 80 | Saline | Saline + CY | Saline |
Ginseng | 100 | Ginseng | Ginseng + CY | Ginseng |
Levamisole | 40 | Levamisole | Levamisole + CY | Levamisole |
PEG6000 | 100 | PEG6000 | PEG6000+CY | PEG6000 |
AJ-PEG | 25 | AJ-PEG | AJ-PEG + CY | AJ-PEG |
50 | AJ-PEG | AJ-PEG + CY | AJ-PEG | |
75 | AJ-PEG | AJ-PEG + CY | AJ-PEG | |
100 | AJ-PEG | AJ-PEG + CY | AJ-PEG |
Gene | Accession No. | Sequence of Primer (5′ to 3′) | |
---|---|---|---|
Forward Primer | Reverse Primer | ||
iNOS | BC062378.1 | TTCCAGAATCCCTGGACAAG | TGGTCAAACTCTTGGGGTTC |
IL-1β | NM_008361.4 | GGGCCTCAAAGGAAAGAATC | TACCAGTTGGGGAACTCTGC |
IL-2 | NM_008366.3 | CCTGAGCAGGATGGAGAATTACA | TCCAGAACATGCCGCAGAG |
IL-4 | NM_021283.2 | ACAGGAGAAGGGACGCCAT | GAAGCCCTACAGACGAGCTCA |
IL-6 | NM_031168.2 | AGTTGCCTTCTTGGGACTGA | CAGAATTGCCATTGCACAAC |
IFN-γ | NM_008337.3 | CTCAAGTGGCATAGATGT | GAGATAATCTGGCTCTGCAGGATT |
TNF-α | D84199.2 | ATGAGCACAGAAAGCATGATC | TACAGGCTTGTCACTCGAATT |
TLR4 | NM_021297.3 | CGCTCTGGCATCATCTTCAT | GTTGCCGTTTCTTGTTCTTCC |
COX-2 | NM_011198.4 | AGAAGGAAATGGCTGCAGAA | GCTCGGCTTCCAGTATTGAG |
β-actin | NM_007393.5 | CCACAGCTGAGAGGGAAATC | AAGGAAGGCTGGAAAAGAGC |
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Choi, J.; Rod-in, W.; Jang, A.-y.; Park, W.J. Arctoscopus japonicus Lipids Enhance Immunity of Mice with Cyclophosphamide-Induced Immunosuppression. Foods 2023, 12, 3292. https://doi.org/10.3390/foods12173292
Choi J, Rod-in W, Jang A-y, Park WJ. Arctoscopus japonicus Lipids Enhance Immunity of Mice with Cyclophosphamide-Induced Immunosuppression. Foods. 2023; 12(17):3292. https://doi.org/10.3390/foods12173292
Chicago/Turabian StyleChoi, JeongUn, Weerawan Rod-in, A-yeong Jang, and Woo Jung Park. 2023. "Arctoscopus japonicus Lipids Enhance Immunity of Mice with Cyclophosphamide-Induced Immunosuppression" Foods 12, no. 17: 3292. https://doi.org/10.3390/foods12173292