Establishment of a Rapid Detection Method for Cadmium Ions via a Specific Cadmium Chelator N-(2-Acetamido)-Iminodiacetic Acid Screened by a Novel Biological Method
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Yeast Strains, Culture Media, Chelators, Growth Assays, and FTIR
2.3. Toxicological Testing of ADA
2.4. Optimization of the Detection System
2.5. Preparation of the Standard Curve
2.6. Sample Preparation for Color Reaction and ICP-MS Measurement
2.7. Statistical Analysis
2.8. Guidelines for Animal Experimentation
3. Results
3.1. A Novel Biological Assay to Test ADA Binding Specificity
3.2. Safety Assessment of ADA
3.3. Identification of Optimal Reaction Conditions
3.4. Spectrum Method for the Detection of Cadmium
3.5. Cadmium Measurements of Agricultural Products by Optimized Spectrum Method
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
ADA | N-(2-Acetamido)-iminodiacetic acid |
VBB | Victoria blue B |
ICP-MS | inductively coupled plasma–mass spectrometry |
MT | metallothionein |
PADs | paper-based analysis devices |
WBC | white blood cells |
Lymph | lymphocytes |
Gran | neutrophils |
RBC | red blood cells |
HGB | hemoglobin |
PLT | platelets |
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Method | Concentration (μg/g) | RSD (%) | Adding Standard Recovery (%) | |
---|---|---|---|---|
Rice | OSM | 0.098 | 4.8 | 70 |
ICP-MS | 0.132 | 4.7 | 98 | |
Bean | OSM | 0.144 | 2.5 | 103 |
ICP-MS | 0.156 | 1.6 | 113 | |
Milk | OSM | 0.112 | 3.3 | 90 |
ICP-MS | 0.101 | 2.2 | 81 | |
Grape | OSM | 0.134 | 1.3 | 106 |
ICP-MS | 0.12 | 0.5 | 94 | |
Peach | OSM | 0.092 | 3.8 | 72 |
ICP-MS | 0.126 | 3.2 | 100 | |
Cabbage | OSM | 0.168 | 1.1 | 120 |
ICP-MS | 0.19 | 1.5 | 138 |
Method | Sensitivity | Specificity | Detection Time | Instrument Aids | Detection Object | Cost | Reference |
---|---|---|---|---|---|---|---|
This method | 0.2 μM | high | 1 min | No | fruits, vegetables, agricultural products | Lower | |
Colorimetric | 0.53 μM | high | 2 min | No | water | Low | [39] |
Nanomaterial | 70 μM | good | Not given | No | aqueous solutions | Low | [40] |
Fluorescence | 0.15 μM | good | 90 min | Yes | aqueous solutions | Medium | [41] |
biosensor | 0.5 μM | medium | Not given | Yes | water | Medium | [42] |
Nanomaterial | 0.23 μM | high | 10 min | No | water | Low | [43] |
Nanomaterial | 20 μM | good | 30 min | No | aqueous solutions | Low | [44] |
Nanomaterial | 0.24 μM | high | 15 min | No | water | Low | [45] |
Nanomaterial | 0.24 μM | high | 70 min | Yes | water | Medium | [46] |
Nanomaterial | 10 μM | high | 10 min | No | rice | Low | [47] |
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Wang, Y.; Sun, W.; Ma, T.; Brake, J.; Zhang, S.; Chen, Y.; Li, J.; Wu, X. Establishment of a Rapid Detection Method for Cadmium Ions via a Specific Cadmium Chelator N-(2-Acetamido)-Iminodiacetic Acid Screened by a Novel Biological Method. Foods 2024, 13, 2684. https://doi.org/10.3390/foods13172684
Wang Y, Sun W, Ma T, Brake J, Zhang S, Chen Y, Li J, Wu X. Establishment of a Rapid Detection Method for Cadmium Ions via a Specific Cadmium Chelator N-(2-Acetamido)-Iminodiacetic Acid Screened by a Novel Biological Method. Foods. 2024; 13(17):2684. https://doi.org/10.3390/foods13172684
Chicago/Turabian StyleWang, Yali, Wenxue Sun, Tinglin Ma, Joseph Brake, Shuangbo Zhang, Yanke Chen, Jing Li, and Xiaobin Wu. 2024. "Establishment of a Rapid Detection Method for Cadmium Ions via a Specific Cadmium Chelator N-(2-Acetamido)-Iminodiacetic Acid Screened by a Novel Biological Method" Foods 13, no. 17: 2684. https://doi.org/10.3390/foods13172684