Rice (Oryza sativa) Stem Cells as a Novel Promising Active Ingredient with Anti-Proliferative Effects for Potential Skin Cancer Prevention and Skin Whitening Activity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Callus and Extraction
2.3. Evaluating Total Phenols and Flavonoids of Callus Extracts
2.4. Radical Scavenging Activity
2.5. Evaluating Total Carbohydrates
2.6. Measuring Total Protein
2.7. Investigation of Presence of Proteins
2.8. Cell Culture
2.9. Detection of Cellular ROS
2.10. Cell Viability Study
2.11. Apoptosis Assay
2.12. DAPI Staining
2.13. Discovery of Autophagic Vacuoles
2.14. Prevention of Levodopa Oxidation
2.15. Melanin Biosynthesis Inhibition in Melanocytes
2.16. Western Blot Analysis
2.17. Statistical Analysis
3. Results
3.1. Preparation and Characterization of Rice Callus Extract
3.2. Antioxidant Capacity Studies
3.3. Studies of Cellular Cytotoxicity of Rice Callus Extracts
3.4. Skin-Whitening Assays
3.5. Western Blot Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Asadollahi, L.; Abbaspour-Ravasjani, S.; Kim, K.A.; Maghsoodi, M.; Hamishehkar, H.; Kosari-Nasab, M.; Kim, K.H. Rice (Oryza sativa) Stem Cells as a Novel Promising Active Ingredient with Anti-Proliferative Effects for Potential Skin Cancer Prevention and Skin Whitening Activity. Foods 2024, 13, 2803. https://doi.org/10.3390/foods13172803
Asadollahi L, Abbaspour-Ravasjani S, Kim KA, Maghsoodi M, Hamishehkar H, Kosari-Nasab M, Kim KH. Rice (Oryza sativa) Stem Cells as a Novel Promising Active Ingredient with Anti-Proliferative Effects for Potential Skin Cancer Prevention and Skin Whitening Activity. Foods. 2024; 13(17):2803. https://doi.org/10.3390/foods13172803
Chicago/Turabian StyleAsadollahi, Leila, Soheil Abbaspour-Ravasjani, Kyung Ah Kim, Maryam Maghsoodi, Hamed Hamishehkar, Morteza Kosari-Nasab, and Ki Hyun Kim. 2024. "Rice (Oryza sativa) Stem Cells as a Novel Promising Active Ingredient with Anti-Proliferative Effects for Potential Skin Cancer Prevention and Skin Whitening Activity" Foods 13, no. 17: 2803. https://doi.org/10.3390/foods13172803