PhoP/PhoQ Two-Component System Contributes to Intestinal Inflammation Induced by Cronobacter sakazakii in Neonatal Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains
2.2. Animal Experiment
2.3. Number of C. sakazakii
2.4. Histopathology
2.5. Caspase-3 Activity
2.6. ELISA
2.7. qRT-PCR
2.8. Immunohistochemical Staining
2.9. Western Blot
2.10. Statistical Analysis
3. Results
3.1. Deletion of phoPQ Gene Reduced the Count of C. sakazaki in Ileum
3.2. PhoP/PhoQ System Affected the Histopathological Damage
3.3. Deletion of phoPQ Gene in C. sakazakii Affected Caspase-3 Activity
3.4. Deletion of phoPQ Gene in C. sakazakii Affected TNF-α and IL-6 Levels in Ileum
3.5. Deletion of phoPQ Gene Affected the Expression of Inflammation-Related Genes
3.6. Deletion of phoPQ Gene Affected the Expression of NF-κB p65
3.7. Deletion of phoPQ Gene in C. sakazakii Affected the Protein Expression of IκBα and TLR4 in the Ileum
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Sequence (5′-3′) | References |
---|---|---|
IL-1β | F: GCAACTGTTCCTGAACTCAACT R: ATCTTTTGGGGTCCGTCAACT | [25] |
TNF-α | F: CCCTCACACTCAGATCATCTTCT R: GCTACGACGTGGGCTACAG | [26] |
IL-6 | F: TAGTCCTTCCTACCCCAATTTCC R: TTGGTCCTTAGCCACTCCTTC | [26] |
NF-κB p65 | F: AGGCTTCTGGGCCTTATGTG R: TGCTTCTCTCGCCAGGAATAC | [27] |
TLR4 | F: ATGGCATGGCTTACACCACC R: GAGGCCAATTTTGTCTCCACA | [28] |
GAPDH | F: AGGTCGGTGTGAACGGATTTG R: TGTAGACCATGTAGTTGAGGTCA |
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Ma, Y.; Zhang, Y.; Wang, Y.; Qiao, Z.; Liu, Y.; Xia, X. PhoP/PhoQ Two-Component System Contributes to Intestinal Inflammation Induced by Cronobacter sakazakii in Neonatal Mice. Foods 2024, 13, 2808. https://doi.org/10.3390/foods13172808
Ma Y, Zhang Y, Wang Y, Qiao Z, Liu Y, Xia X. PhoP/PhoQ Two-Component System Contributes to Intestinal Inflammation Induced by Cronobacter sakazakii in Neonatal Mice. Foods. 2024; 13(17):2808. https://doi.org/10.3390/foods13172808
Chicago/Turabian StyleMa, Yan, Yingying Zhang, Yuting Wang, Zhu Qiao, Yingying Liu, and Xiaodong Xia. 2024. "PhoP/PhoQ Two-Component System Contributes to Intestinal Inflammation Induced by Cronobacter sakazakii in Neonatal Mice" Foods 13, no. 17: 2808. https://doi.org/10.3390/foods13172808