Heterotrophic Selenium Incorporation into Chlorella vulgaris K-01: Selenium Tolerance, Assimilation, and Removal through Microalgal Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Heterotrophic Culture and Selenium Treatment
2.2. Measurement of Microalgal Biomass
2.3. Growth Parameters Calculation
2.4. Gaussian Process (GP) Regression Analysis
2.5. Glucose Detection and Evaluation
2.6. SEM Microscopy
2.7. Intracellular Selenium Detection through ICP-MS
2.8. Extracellular Residual Selenium Determination through ICP-OES
2.9. Statistical Analysis
3. Results and Discussion
3.1. Se Dose–Response of Chlorella vulgaris K-01 under Heterotrophic Medium
3.2. Growth Rate Analysis
3.3. Growth Curves Analysis Using GP Regression
3.4. Microalgal Morphology Disruption under Se Stress
3.5. Glucose Consumption Analysis
3.6. Se Incorporation and Removal
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhang, Z.; Zhang, Y.; Hua, Y.; Chen, G.; Fu, P.; Liu, J. Heterotrophic Selenium Incorporation into Chlorella vulgaris K-01: Selenium Tolerance, Assimilation, and Removal through Microalgal Cells. Foods 2024, 13, 405. https://doi.org/10.3390/foods13030405
Zhang Z, Zhang Y, Hua Y, Chen G, Fu P, Liu J. Heterotrophic Selenium Incorporation into Chlorella vulgaris K-01: Selenium Tolerance, Assimilation, and Removal through Microalgal Cells. Foods. 2024; 13(3):405. https://doi.org/10.3390/foods13030405
Chicago/Turabian StyleZhang, Zhenyu, Yan Zhang, Yanying Hua, Guancheng Chen, Pengcheng Fu, and Jing Liu. 2024. "Heterotrophic Selenium Incorporation into Chlorella vulgaris K-01: Selenium Tolerance, Assimilation, and Removal through Microalgal Cells" Foods 13, no. 3: 405. https://doi.org/10.3390/foods13030405