Isolation and Characterisation of Streptococcus spp. with Human Milk Oligosaccharides Utilization Capacity from Human Milk
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Cultivation of the Strains
2.2. Taxonomic Identification
2.3. HMO Fermentation and Growth Curve
2.4. HMO Consumption and Metabolite Quantitation
2.5. Genome Mining Analysis
2.6. Haemolysis Activity
3. Results
3.1. Isolation of Streptococcus spp. with HMO Utilization Capability
3.2. Characteristics of HMO Utilization by Streptococcus
3.3. In Silico and In Vitro Safety Assessment of the Three Undefined Streptococci
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain | VFDB ID | Gene Name | VFs | Identity (%) | Description |
---|---|---|---|---|---|
21WXBC0057M1 | VFG001359 | psaA | PsaA | 91 | Manganese ABC transporter |
VFG005197 | pavA | PavA | 89.4 | Adherence and virulence protein | |
VFG000964 | hasC | Hyaluronic acid capsule | 86.8 | Prevents phagocytosis | |
VFG001365-VFG001368 | cps4A, cps4B, cpsC, cps4D | Capsule | 73.5–74.7 | Resistant to complement deposition | |
21WXBC0044M1 | VFG042972 VFG042974 | sipA, srtG1 | PI-2 | >87.7 | Mediates host cell adhesion |
VFG005197 | pavA | PavA | 94.2 | Adherence and virulence protein | |
VFG001359 | psaA | PsaA | 93.2 | Manganese ABC transporter | |
VFG001365- VFG001368 | cps4A, cps4B, cpsC, cps4D | Capsule | 86.4 | Resistant to complement | |
VFG000964 | hasC | Hyaluronic acid capsule | 79.1 | Prevents phagocytosis | |
VFG048830 | gndA | Capsule | 70.6 | Protects bacteria from opsonophagocytosis | |
BJSWXB5TM5 | VFG001359 | psaA | PsaA | 93.5 | Manganese ABC transporter |
VFG005197 | pavA | PavA | 89.4 | Adherence and virulence protein | |
VFG000964 | hasC | Hyaluronic acid capsule | 86.5 | Prevents phagocytosis | |
VFG001378 | / | Neuraminidase | 70 | Contributes to increased adhesion |
Strain Number | Length | Gene Numbers | The Most Common Prophage | GC Content (%) |
---|---|---|---|---|
21WXBC0057M1 | 32.1Kb | 140 | PHAGE_Strept_PH15_NC_010945(11) | 41.42 |
BJSWXB5TM5 | 58.7Kb | 140 | PHAGE_Strept_phi3396_NC_009018(10) | 40.25 |
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Zhou, Y.; Liu, X.; Chen, H.; Zhao, J.; Zhang, H.; Chen, W.; Yang, B. Isolation and Characterisation of Streptococcus spp. with Human Milk Oligosaccharides Utilization Capacity from Human Milk. Foods 2024, 13, 1291. https://doi.org/10.3390/foods13091291
Zhou Y, Liu X, Chen H, Zhao J, Zhang H, Chen W, Yang B. Isolation and Characterisation of Streptococcus spp. with Human Milk Oligosaccharides Utilization Capacity from Human Milk. Foods. 2024; 13(9):1291. https://doi.org/10.3390/foods13091291
Chicago/Turabian StyleZhou, Ye, Xiaoming Liu, Haiqin Chen, Jianxin Zhao, Hao Zhang, Wei Chen, and Bo Yang. 2024. "Isolation and Characterisation of Streptococcus spp. with Human Milk Oligosaccharides Utilization Capacity from Human Milk" Foods 13, no. 9: 1291. https://doi.org/10.3390/foods13091291