Insight into the Effects of Sous Vide on Cathepsin B and L Activities, Protein Degradation and the Ultrastructure of Beef
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Preparation
2.2. Cth B and Cth L Activity
2.3. Collagen Solubility
2.4. Preparation of Intramuscular Connective Tissue
2.5. Differential Scanning Calorimetry (DSC)
2.6. Scanning Electron Microscope (SEM)
2.7. Preparation of Myofibrillar Protein
2.8. Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
2.9. Myofibril Fragmentation Index (MFI)
2.10. Ultrastructure and Sarcomere Length
2.11. Shear Force
2.12. Statistical Analysis
3. Results and Discussion
3.1. Cth B and Cth L Activities in Exudate
3.2. Cth B and Cth L Activities in Muscle
3.3. Change in Collagen
3.3.1. DSC
3.3.2. Collagen Solubility
3.3.3. SEM
3.4. SDS-PAGE and MFI
3.5. Ultrastructure of Myofibre and Sarcomere Length
3.6. Shear Force
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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CK | SV45 | SV55 | SV65 | |
---|---|---|---|---|
Collagen solubility (%) | 3.54 ± 0.11 c | 3.74 ± 0.17 c | 4.49 ± 0.19 a | 3.96 ± 0.10 b |
MFI | 35.37 ± 1.66 c | 44.83 ± 1.67 a | 47.66 ± 2.42 a | 39.67 ± 2.47 b |
Shear force (N/cm2) | 62.34 ± 2.50 a | 55.28 ± 1.31 b | 46.69 ± 1.47 c | 52.84 ± 1.61 b |
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Yin, Y.; Pereira, J.; Zhou, L.; Lorenzo, J.M.; Tian, X.; Zhang, W. Insight into the Effects of Sous Vide on Cathepsin B and L Activities, Protein Degradation and the Ultrastructure of Beef. Foods 2020, 9, 1441. https://doi.org/10.3390/foods9101441
Yin Y, Pereira J, Zhou L, Lorenzo JM, Tian X, Zhang W. Insight into the Effects of Sous Vide on Cathepsin B and L Activities, Protein Degradation and the Ultrastructure of Beef. Foods. 2020; 9(10):1441. https://doi.org/10.3390/foods9101441
Chicago/Turabian StyleYin, Yantao, Jailson Pereira, Lei Zhou, Jose M. Lorenzo, Xiaona Tian, and Wangang Zhang. 2020. "Insight into the Effects of Sous Vide on Cathepsin B and L Activities, Protein Degradation and the Ultrastructure of Beef" Foods 9, no. 10: 1441. https://doi.org/10.3390/foods9101441