The Effect of Nanocoatings Enriched with Essential Oils on ‘Rocha’ Pear Long Storage
Abstract
:1. Introduction
2. Material and Methods
2.1. Chemicals
2.2. Lemongrass Essential Oil (LG) Analysis
2.3. Preparation of Coating-Forming Nanoemulsions
2.4. Nanoemulsion Characterization
2.5. Fruit Coating
2.6. Quality Parameters
2.7. Microbial Counts
2.8. Sensory Evaluation
2.9. Statistical Analysis
3. Results and Discussion
3.1. Lemongrass Essential Oil (LG) Composition
3.2. Nanoemulsion Characterization
3.2.1. Droplet Size, Polydispersity and Zeta-Potential
3.2.2. Microstructure
3.2.3. Nanoemulsions Cytotoxicity
3.3. Fruit Quality Parameters
3.3.1. Colour
3.3.2. Firmness
3.3.3. SSC and TA
3.3.4. Electrolyte Leakage (EL)
3.3.5. Weight Loss
3.3.6. Aerobic Mesophilic Microorganisms and Yeasts and Moulds
3.3.7. Superficial Scald and Internal Browning
3.3.8. Sensory Evaluation
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Compounds | RI | % |
---|---|---|
Tricyclene | 921 | 0.4 |
α-Fenchene | 938 | t |
Camphene | 938 | 1.7 |
6-Methylhept-5-en-2-one | 960 | 1.0 |
β-Myrcene | 975 | 0.3 |
1,8-Cineole | 1005 | 0.4 |
Limonene | 1009 | 0.4 |
Linalool | 1074 | 1.1 |
Citronellal | 1121 | 0.4 |
α-Terpineol | 1159 | 0.3 |
Neral | 1210 | 34.8 |
Piperitone | 1211 | 0.1 |
Geraniol | 1236 | 16.9 |
Geranial | 1240 | 33.7 |
Geranyl acetate | 1370 | 3.2 |
β-Caryophyllene | 1414 | 1.2 |
trans-α-Bergamotene | 1434 | t |
α-Humulene | 1447 | 0.1 |
δ-Cadinene | 1505 | 0.1 |
Elemol | 1530 | 0.3 |
β-Caryophyllene oxide | 1561 | 0.4 |
% of Identification | 96.8 | |
Grouped compounds | ||
Monoterpene hydrocarbons | 2.8 | |
Oxygen-containing monoterpenes | 90.9 | |
Sesquiterpene hydrocarbons | 1.4 | |
Oxygen-containing sesquiterpenes | 0.7 | |
Others | 1.0 |
Nanoemulsion | Droplet Size (nm) | Polydispersity Index | Zeta-Potential (mV) |
---|---|---|---|
Cit1% | 42.15 ± 11.42 bc | 0.36 ± 0.03 a | −52.84 ± 3.18 c |
Cit2% | 26.04 ± 5.43 c | 0.31 ± 0.04 ab | −37.79 ± 8.53 b |
LG1.25% | 82.81 ± 39.28 a | 0.36 ± 0.04 a | −28.29 ± 10.69 ab |
LG2.5% | 68.28 ± 21.84 ab | 0.26 ± 0.05 b | −23.10 ± 6.53 a |
Quality Parameters | 0 Month | 2 Months | 4 Months | 6 Months | ||||
---|---|---|---|---|---|---|---|---|
Nanoemulsion | 0 d | 7 d | 0 d | 7 d | 0 d | 7 d | 0 d | 7 d |
Lightness (L*) | ||||||||
Cit1% | 71.02 ± 0.52 aD | 71.17 ± 0.49 aD | 74.03 ± 0.49 cC | 74.91 ± 0.45 bBC | 75.45 ± 0.50 cB | 75.56 ± 0.62 bB | 77.31 ± 0.35 aA | 77.87 ± 0.21 abA |
Cit2% | 70.36 ± 0.37 aC | 70.42 ± 0.41 abDC | 73.61 ±.62 cB | 73.76 ± 0.55 bcB | 76.98 ± 0.25 bcA | 77.43 ± 1.21 abA | 78.04 ± 0.27 aA | 77.04 ± 0.31 bA |
LG1.25% | 71.16 ± 0.39 aD | 70.30 ± 0.42 abD | 75.64 ± 0.29 bB | 73.13 ± 0.61 cdC | 76.10 ± 0.55 bcB | 76.01 ± 0.56 abB | 78.04 ± 0.31 aA | 77.71 ± 0.37 abA |
LG2.5% | 70.80 ± 0.45 aCD | 69.41 ± 0.57 bD | 75.06 ± 0.62 bcB | 72.08 ± 0.54 dC | 76.50 ± 0.65 bcA | 76.76 ± 0.34 abA | 77.79 ± 0.32 aA | 77.76 ± 0.37 abA |
Control | 71.39 ± 0.58 aB | 71.45 ± 0.49 aB | 77.30 ± 0.39 aA | 78.20 ± 0.39 aA | 78.02 ± 0.30 aA | 77.90 ± 0.29 aA | 77.62 ± 0.25 aA | 78.02 ± 0.25 aA |
Hue angle (h) | ||||||||
Cit1% | 108.86 ± 0.71 aA | 107.33 ± 0.61 bcA | 105.44 ± 0.53 aB | 103.04 ± 0.80 aC | 100.35 ± 0.62 aD | 95.85 ± 0.73 aE | 93.77 ± 0.53 aF | 94.42 ± 0.31 aEF |
Cit2% | 109.96 ± 0.53 aA | 109.43 ± 0.49 aA | 105.14 ± 0.63 abB | 103.05 ± 0.63 aC | 98.30 ± 0.44 bD | 96.18 ± 0.61 aE | 94.08 ± 0.33 abF | 92.79 ± 0.36 bF |
LG1.25% | 109.59 ± 0.53 aA | 108.87 ± 0.44 abA | 103.54 ± 0.49 bcB | 104.02 ± 0.62 aB | 97.98 ± 0.55 bC | 97.00 ± 0.56 aC | 93.10 ± 0.47 abD | 93.42 ± 0.37 bD |
LG2.5% | 109.83 ± 0.49 aA | 108.91 ± 0.58 abA | 102.73 ± 0.58 cC | 104.31 ± 0.39 aB | 96.37 ± 0.49 cD | 95.79 ± 0.36 aD | 91.66 ± 0.60 cE | 92.52 ± 0.29 bE |
Control | 108.76 ± 0.70 aA | 106.20 ± 0.99 cB | 98.35 ± 0.69 dC | 93.21 ± 0.36 bDE | 94.70 ± 0.28 dD | 91.48 ± 0.31 bE | 92.36 ± 0.36 bcE | 91.59 ± 0.24 cE |
Firmness (N) | ||||||||
Cit1% | 52.01 ± 1.98 aA | 39.83 ± 2.93 abC | 45.98 ± 2.45 bABC | 19.18 ± 3.55 abD | 48.22 ± 1.32 aAB | 14.95 ± 3.34 bcD | 43.74 ± 1.16 abBC | 19.18 ± 2.22 abD |
Cit2% | 51.19 ± 1.88 aA | 45.37 ± 2.25 aB | 51.68 ± 1.39 aA | 15.45 ± 3.18 bcC | 46.35 ± 0.91 abAB | 14.96 ± 2.41 bcC | 44.26 ± 1.35 aB | 14.42 ± 1.45 bcC |
LG1.25% | 47.34 ± 1.32 aA | 40.89 ± 2.56 abA | 46.04 ± 0.97 bA | 22.54 ± 3.31 abB | 45.28 ± 1.64 abA | 23.72 ± 3.20 aB | 40.73 ± 1.05 bcA | 21.01 ± 1.78 aB |
LG2.5% | 48.98 ± 1.77 aA | 37.57 ± 3.53 abC | 45.30 ± 1.65 bAB | 26.94 ± 3.64 aD | 43.69 ± 0.87 bcABC | 21.13 ± 2.22 abD | 39.44 ± 1.15 cBC | 20.41 ± 2.75 aD |
Control | 49.83 ± 2.24 aA | 33.47 ± 4.79 bC | 39.95 ± 2.45 cB | 7.63 ± 0.50 cD | 40.68 ± 1.21 cB | 7.78 ± 0.81 cD | 40.73 ± 0.91 bcB | 9.63 ± 0.53 cD |
SSC (%) | ||||||||
Cit1% | 12.63 ± 0.09 abB | 13.63 ± 0.28 bcA | 13.43 ± 0.19 bAB | 14.10 ± 0.21 aA | 13.93 ± 0.12 aA | 14.07 ± 0.29 aA | 13.87 ± 0.17 aA | 13.97 ± 0.21 aA |
Cit2% | 12.23 ± 0.07 bD | 13.17 ± 0.29 cBC | 13.43 ± 0.09 bBC | 13.83 ± 0.09 aAB | 13.43 ± 0.09 aBC | 13.87 ± 0.24 aAB | 14.43 ± 0.38 aA | 12.97 ± 0.15 bC |
LG1.25% | 12.33 ± 0.19 bB | 13.87 ± 0.18 bcA | 14.07 ± 0. 07 aA | 14.40 ± 0.35 aA | 14.30 ± 0.25 aA | 14.47 ± 0.21 aA | 14.30 ± 0.28 aA | 13.80 ± 0.29 abA |
LG2.5% | 12.63 ± 0.23 abB | 14.17 ± 0.17 abA | 14.30 ± 0.15 aA | 14.10 ± 0.26 aA | 14.10 ± 0.37 aA | 14.40 ± 0.26 aA | 14.40 ± 0.21 aA | 13.63 ± 0.35 abA |
Control | 12.97 ± 0.27 aC | 14.70 ± 0.3 aA | 14.13 ± 0.17 aAB | 13.80 ± 0.10 aABC | 14.20 ± 0.41 aAB | 13.70 ± 0.25 aBC | 14.03 ± 0.32 aAB | 13.27 ± 0.32 abBC |
Titratable acidity (g.mL−1 malic acid) | ||||||||
Cit1% | 0.42 ± 0.03 aA | 0.18 ± 0.01 abB | 0.16 ± 0.00 aB | 0.16 ± 0.00 abB | 0.13 ± 0.01 aC | 0.10 ± 0.00 abC | 0.10 ± 0.01 cC | 0.11 ± 0.00 aC |
Cit2% | 0.43 ± 0.06 aA | 0.20 ± 0.01 aB | 0.15 ± 0.01 abBC | 0.18 ± 0.01 aBC | 0.11 ± 0.01 aC | 0.11 ± 0.00 abC | 0.15 ± 0.01 aBC | 0.12 ± 0.01 aC |
LG1.25% | 0.39 ± 0.00 aA | 0.15 ± 0.01 bBC | 0.14 ± 0.01 bBC | 0.16 ± 0.01 abB | 0.12 ± 0.01 aD | 0.13 ± 0.00 aCD | 0.14 ± 0.01 abBCD | 0.12 ± 0.00 aD |
LG2.5% | 0.45 ± 0.03 aA | 0.19 ± 0.01 aB | 0.15 ± 0.01 abC | 0.14 ± 0.00 bC | 0.12 ± 0.01 aC | 0.12 ± 0.01 aC | 0.12 ± 0.02 abcC | 0.13 ± 0.00 aC |
Control | 0.49 ± 0.02 aA | 0.17 ± 0.01 abcB | 0.14 ± 0.00 bBC | 0.16 ± 0.01 abBC | 0.14 ± 0.01 aBC | 0.09 ± 0.02 bD | 0.11 ± 0.00 bcCD | 0.11 ± 0.00 aCD |
Electrolytic leakage (%) | ||||||||
Cit1% | 54.23 ± 0.79 aC | 58.15 ± 0.80 aC | 42.41 ± 1.39 bE | 69.40 ± 4.02 aB | 46.36 ± 1.95 bDE | 69.59 ± 3.63 bB | 52.72 ± 0.77 bCD | 76.49 ± 0.34 bA |
Cit2% | 49.12 ± 1.41 aC | 59.60 ± 1.77 aB | 41.82 ± 1.02 bC | 69.62 ± 2.65 aA | 45.35 ± 1.21 bC | 68.60 ± 1.58 bA | 48.10 ± 2.67 bcC | 75.37 ± 6.74 bA |
LG1.25% | 49.66 ± 1.24 aCD | 56.07 ± 2.17 aBC | 41.92 ± 1.30 bE | 61.02 ± 3.32 abB | 43.2 ± 1.34 bDE | 54.89 ± 0.82 cBC | 43.51 ± 1.83 cDE | 71.39 ± 3.04 bA |
LG2.5% | 51.44 ± 2.17 aBC | 55.49 ± 0.34 aBC | 40.38 ± 3.02 bE | 58.92 ± 1.21 bB | 42.39 ± 1.07 bDE | 53.85 ± 4.38 cBC | 48.36 ± 2.09 bcCDE | 71.33 ± 2.03 bA |
Control | 50.56 ± 2.62 aC | 60.12 ± 3.76 aB | 49.43 ± 3.25 aC | 62.60 ± 3.04 abB | 55.09 ± 2.10 aBC | 83.04 ± 1.51 aA | 59.83 ± 1.61 aB | 88.26 ± 1.88 aA |
Weight loss (%) | ||||||||
Cit1% | 0.00 ± 0.00 aF | 1.66 ± 0.03 abE | 2.08 ± 0.05 cE | 3.63 ± 0.05 cD | 4.34 ± 0.29 bC | 6.66 ± 0.24 cB | 6.57 ± 0.33 bB | 7.64 ± 0.39 bA |
Cit2% | 0.00 ± 0.00 aH | 1.65 ± 0.03 abG | 2.23 ± 0.03 cF | 3.96 ± 0.03 bE | 4.40 ± 0.06 bD | 6.99 ± 0.18 bcB | 6.63 ± 0.05 bC | 7.84 ± 0.02 abA |
LG1.25% | 0.00 ± 0.00 aG | 1.70 ± 0.05 aF | 2.49 ± 0.06 bE | 4.13 ± 0.06 bD | 5.00 ± 0.17 aB | 7.42 ± 0.20 abB | 7.36 ± 0.25 aB | 8.45 ± 0.28 abA |
LG2.5% | 0.00 ± 0.00 aG | 1.80 ± 0.09 aF | 2.90 ± 0.08 aE | 4.44 ± 0.17 aD | 5.54 ± 0.20 aC | 7.93 ± 0.21 aB | 7.53 ± 0.19 aB | 8.59 ± 0.22 aA |
Control | 0.00 ± 0.00 aG | 1.51 ± 0.04 bF | 2.08 ± 0.03 cE | 3.42 ± 0.02 cD | 4.25 ± 0.07 bC | 6.56 ± 0.07 cB | 6.72 ± 0.08 bB | 7.8 ± 0.11 abA |
Microorganisms | 0 Months | 2 Months | 4 Months | 6 Months | ||||
---|---|---|---|---|---|---|---|---|
Nanoemulsion | 0 d | 7 d | 0 d | 7 d | 0 d | 7 d | 0 d | 7 d |
Yeast and moulds (Log CFU/g) | ||||||||
Cit 1% | 0.67 ± 0.33 aA | 0.33 ± 0.33 aA | 0.67 ± 0.33 aA | 0.67 ± 0.33 abA | 0.93 ± 0.47 abA | 1.00 ± 0.00 aA | 0.41 ± 0.22 aA | 0.33 ± 0.33 aA |
Cit 2% | 0.77 ± 0.39 aA | 0.33 ± 0.33 aA | 0.77 ± 0.39 aA | 0.33 ± 0.33 bA | 0.33 ± 0.33 bA | 0.00 ± 0.00 bA | 0.17 ± 0.17 aAB | 0.00 ± 0.00 aA |
LG 1.25 % | 1.00 ± 0.00 aA | 0.00 ± 0.00 aB | 1.00 ± 0.00 aA | 0.00 ± 0.00 bB | 0.67 ± 0.33 bAB | 0.77 ± 0.39 aAB | 0.38 ± 0.20 aBC | 0.43 ± 0.43 aAB |
LG 2.5 % | 0.93 ± 0.47 aA | 0.00 ± 0.00 aB | 1.10 ± 0.10 aA | 0.00 ± 0.00 bB | 0.00 ± 0.00 bB | 0.00 ± 0.00 bB | 0.33 ± 0.17 aAB | 0.00 ± 0.00 aB |
Control | 1.10 ±0.10 aBC | 0.83 ± 0.44 aBC | 1.20 ± 0.10 aB | 1.10 ± 0.10 aBC | 1.77 ± 0.04 aA | 1.26 ± 0.14 aAB | 0.58 ± 0.08 aD | 0.00 ± 0.00 aD |
Aerobic mesophilic Microorganisms (Log CFU/g) | ||||||||
Cit 1% | 3.72 ± 0.03 aA | 2.67 ± 0.11 bB | 1.32 ± 0.02 cC | 2.54 ± 0.16 aB | 1.72 ± 0.14 bcDE | 3.52 ± 0.09 aA | 2.44 ± 0.61 aB | 3.51 ± 0.04 aA |
Cit 2% | 3.59 ± 0.02 aAB | 3.87 ± 0.02 aA | 2.38 ± 0.52 bCD | 1.19 ± 0.27 bE | 1.59 ± 0.19 cDE | 2.81 ± 0.05 cBC | 3.18 ± 0.60 aABC | 3.30 ± 0.17 aABC |
LG 1.25% | 3.60 ± 0.06 aA | 0.71 ± 0.03 dF | 1.32 ± 0.09 cEF | 2.71 ± 0.07 aBC | 2.30 ± 0.20 abCD | 3.74 ± 0.06 aA | 3.26 ± 0.14 aAB | 1.94 ± 0.51 bDE |
LG 2.5% | 3.44 ± 0.06 bAB | 0.68 ± 0.03 dE | 1.18 ± 0.09 cDE | 2.54 ± 0.02 aBC | 2.04 ± 0.28 abcCD | 3.54 ± 0.02 bA | 2.65 ± 0.51 aABC | 2.70 ± 0.56 abABC |
Control | 2.85 ± 0.05 cC | 2.08 ± 0.06 cE | 3.69 ± 0.05 aA | 2.50 ± 0.02 aD | 2.45 ± 0.11 aD | 3.50 ± 0.02 bB | 3.65 ± 0.00 aAB | 3.00 ± 0.03 abC |
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Gago, C.; Antão, R.; Dores, C.; Guerreiro, A.; Miguel, M.G.; Faleiro, M.L.; Figueiredo, A.C.; Antunes, M.D. The Effect of Nanocoatings Enriched with Essential Oils on ‘Rocha’ Pear Long Storage. Foods 2020, 9, 240. https://doi.org/10.3390/foods9020240
Gago C, Antão R, Dores C, Guerreiro A, Miguel MG, Faleiro ML, Figueiredo AC, Antunes MD. The Effect of Nanocoatings Enriched with Essential Oils on ‘Rocha’ Pear Long Storage. Foods. 2020; 9(2):240. https://doi.org/10.3390/foods9020240
Chicago/Turabian StyleGago, Custódia, Rui Antão, Cristino Dores, Adriana Guerreiro, Maria Graça Miguel, Maria Leonor Faleiro, Ana Cristina Figueiredo, and Maria Dulce Antunes. 2020. "The Effect of Nanocoatings Enriched with Essential Oils on ‘Rocha’ Pear Long Storage" Foods 9, no. 2: 240. https://doi.org/10.3390/foods9020240