Effects of Farm Management Practices and Transport Time on Post-Mortem Changes of Longissimus lumborum Muscle Proteins in Suckling Goat Kids
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Handling and Slaughtering
2.2. Experimental Design
2.3. Extraction of Sarcoplasmic (SPP) and Myofibrillar (MFP) Fractions
2.4. Determination of Soluble Protein
2.5. Total Amino Acid Determination
2.6. Electrophoresis of Muscle Proteins
2.7. Densitometric Analysis
2.8. Statistical Analysis
3. Results
4. Discussion
4.1. Proteins and Aminoacids Content
4.2. Electrophoresis of Sarcoplasmic Proteins
4.3. Electrophoresis of Myofibrillar Proteins
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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ST | LT | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Farm Origin | Post-Mortem Time (days) | Post-Mortem Time (days) | FO | TD | AT | FO*TD | FO*AT | TD*AT | FO*TD*AT | |||||
3 | 8 | 21 | 3 | 8 | 21 | |||||||||
B-97 kDa | GW | 5.07 b ± 0.57 | 4.59 b ± 0.51 | 2.66 a ± 0.33 | 7.84 c# ± 0.45 | 5.35 b ± 0.46 | 1.89 a ± 0.51 | * | ns | *** | ns | * | ns | * |
DW | 6.05 b ± 0.57 | 4.13 ab ± 0.51 | 3.03 a ± 0.33 | 5.21 b* ± 0.45 | 3.72 ab* ± 0.46 | 3.16 a ± 0.51 | ||||||||
B-65 kDa | GW | 5.77 ± 0.53 | 5.52 ± 0.58 | 5.99 ± 0.47 | 7.02 ± 0.40 | 7.07 ± 0.38 | 6.38 ± 0.40 | ns | * | ns | ns | ns | ns | ns |
DW | 5.44 ± 0.53 | 6.13 ± 0.58 | 7.14 ± 0.47 | 6.59 ± 0.40 | 6.36 ± 0.38 | 6.77 ± 0.40 | ||||||||
B-58 kDa | GW | 8.34 ± 0.17 | 7.45 ± 0.68 | 8.48 ± 0.19 | 7.66 a ± 0.69 | 9.24 b ± 0.58 | 9.88 b# ± 0.48 | ns | ns | ns | * | ns | ns | ns |
DW | 8.24 ± 0.19 | 9.04 ± 0.68 | 8.44 ± 0.22 | 8.47 ± 0.69 | 7.81 ± 0.58 | 8.51 ± 0.48 | ||||||||
B-46 kDa | GW | 10.25 ± 0.49 | 9.07 ± 0.50 | 9.71 ± 0.46 | 9.75 ± 0.49 | 10.09 ± 0.73 | 11.85 # ± 0.62 | ** | ns | * | * | ns | ns | ns |
DW | 11.00 ± 0.49 | 11.12 * ± 0.50 | 12.49 * ± 0.46 | 10.17 ± 0.49 | 10.54 ± 0.73 | 11.40 ± 0.62 | ||||||||
B-43 kDa | GW | 23.45 ± 0.81 | 21.43 ± 1.08 | 20.80 ± 0.32 | 21.37 ± 1.54 | 22.17 ± 1.48 | 19.06 ± 0.58 | ns | ns | ns | ns | ns | ns | ns |
DW | 25.84 ± 0.93 | 22.95 ± 1.08 | 21.59 ± 0.28 | 21.91 ± 1.54 | 21.09 ± 1.48 | 22.56 #* ± 0.58 | ||||||||
B-40 kDa | GW | 11.88 ± 1.13 | 11.61 ± 0.91 | 13.93 ± 1.64 | 12.14 a ± 1.02 | 11.60 a ± 0.85 | 15.74 b ± 0.65 | ns | ns | ns | ns | ns | ns | ns |
DW | 12.07 ± 1.13 | 10.81 ± 0.91 | 10.74 ± 1.64 | 11.60 ± 1.02 | 11.66 ± 0.85 | 12.17 * ± 0.65 | ||||||||
B-38 kDa | GW | 6.59 c ± 0.66 | 4.47 b ± 0.27 | 1.74 a ± 0.24 | 6.51 b ± 0.46 | 5.45 b ± 0.54 | 1.52 a ± 0.40 | ** | ns | *** | ns | ** | ns | ns |
DW | 7.37 c ± 0.66 | 5.88 b* ± 0.24 | 3.38 a* ± 0.24 | 6.14 c ± 0.40 | 5.20 ab ± 0.54 | 3.98 a* ± 0.40 | ||||||||
B-36 kDa | GW | 7.47 ± 0.59 | 7.50 ± 0.70 | 7.48 ± 0.52 | 5.73 a ± 0.47 | 6.48 a ± 0.49 | 8.54 b ± 0.50 | * | ns | ns | ns | ns | ns | ns |
DW | 6.64 ± 0.59 | 6.84 ± 0.70 | 6.48 ± 0.52 | 6.13 ± 0.40 | 6.16 ± 0.49 | 6.78 ± 0.50 | ||||||||
B-30 kDa | GW | 1.96 ± 0.67 | 2.17 ± 0.37 | 2.41 ± 0.26 | 2.33 ± 0.17 | 2.75 ± 0.38 | 2.16 ± 0.38 | ns | ns | ns | ns | ns | ns | ns |
DW | 2.10 ± 0.67 | 2.05 ± 0.37 | 2.24 ± 0.26 | 2.39 ± 0.15 | 2.94 ± 0.38 | 2.33 ± 0.38 | ||||||||
B-28 kDa | GW | 4.33 ± 0.38 | 5.41 ± 0.25 | 4.45 ± 0.18 | 4.14 ± 0.26 | 4.60 ± 0.32 | 5.02 ± 0.50 | ns | ns | ns | ns | ns | ns | ns |
DW | 4.75 ± 0.38 | 4.70 ± 0.21 | 4.75 ± 0.15 | 5.01 ± 0.26 | 4.91 ± 0.28 | 5.36 ± 0.50 | ||||||||
B-26 kDa | GW | 2.25 a ± 0.29 | 4.33 ab ± 0.30 | 4.56 b ± 0.46 | 2.58 a ± 0.31 | 3.39 b ± 0.39 | 4.80 b ± 0.47 | ** | ns | *** | ns | ns | ns | ns |
DW | 2.05 a ± 0.29 | 2.71 a* ± 0.26 | 3.98 b ± 0.40 | 2.23 a ± 0.27 | 3.58 b ± 0.34 | 3.74 b ± 0.47 | ||||||||
B-16 kDa | GW | 2.67 ± 0.48 | 3.04 ± 0.24 | 2.10 ± 0.44 | 3.17 ± 0.32 | 3.54 ± 0.41 | 2.54 ± 0.39 | ns | ns | ns | ns | ns | ns | ns |
DW | 2.75 ± 0.48 | 2.30 ± 0.21 | 2.83 ± 0.44 | 3.11 ± 0.32 | 2.56 ± 0.41 | 2.47 ± 0.39 |
ST | LT | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Farm Origin | Post-Mortem Time (days) | Post-Mortem Time (days) | FO | TD | AT | FO*TD | FO*AT | TD*AT | FO*TD*AT | |||||
3 | 8 | 21 | 3 | 8 | 21 | |||||||||
MHC † | GW | 31.05 ± 0.65 | 30.48 ± 0.53 | 31.42 ± 0.91 | 31.76 ± 0.23 | 31.25 ± 0.26 | 30.40 ± 0.56 | ns | ns | ns | ns | ns | ns | ns |
DW | 30.83 ± 0.65 | 29.87 ± 0.62 | 29.74 ± 0.91 | 31.05 b ± 0.26 | 29.99 a ± 0.22 | 29.82 a ± 0.56 | ||||||||
Protein C ‡ | GW | 1.42 ± 0.32 | 1.11 ± 0.26 | 1.11 ± 0.23 | 1.91 ± 0.20 | 2.05 ± 0.41 | 1.93 ± 0.12 | ns | ** | ns | ns | ns | ns | ns |
DW | 1.66 ± 0.32 | 0.85 ± 0.30 | 0.97 ± 0.23 | 1.64 * ± 0.20 | 1.12 * ± 0.41 | 1.19 * ± 0.12 | ||||||||
α-actinin § | GW | 2.89 ± 0.27 | 2.55 ± 0.35 | 2.38 ± 0.21 | 2.65 ± 0.20 | 2.32 ± 0.16 | 2.41 ± 0.18 | ns | ns | ns | ns | ns | ns | ns |
DW | 1.88 ± 0.27 | 2.04 ± 0.35 | 2.67 ± 0.21 | 2.51 ± 0.20 | 2.74 ± 0.29 | 2.07 ± 0.18 | ||||||||
Desmin | GW | 2.75 ± 0.17 | 1.98 ± 0.13 | 2.21 ± 0.28 | 2.39 ± 0.13 | 1.53 ± 0.32 | 2.07 ± 0.24 | * | ns | ns | ns | ns | ns | ns |
DW | 1.99 * ± 0.17 | 1.63 * ± 0.11 | 1.45 * ± 0.28 | 1.71 * ± 0.23 | 1.58 ± 0.32 | 1.55 ± 0.24 | ||||||||
Actin ϒ | GW | 31.03 ± 0.23 | 31.08 ± 0.60 | 30.28 ± 0.32 | 31.77 ± 0.34 | 30.97 ± 0.53 | 31.16 ± 0.30 | ns | ns | ns | ns | ns | ns | ns |
DW | 30.59 ± 0.27 | 30.50 ± 0.60 | 29.40 ± 0.32 | 30.91 ± 0.34 | 29.88 ± 0.53 | 29.07 ± 0.26 | ||||||||
TnT Ϯ | GW | 8.47 b ± 0.69 | 5.94 a ± 0.69 | 6.04 a ± 0.69 | 7.94 b ± 0.36 | 6.68 a ± 0.36 | 6.10 a ± 0.36 | *** | ns | *** | ns | ns | ns | ns |
DW | 5.85 b* ± 0.45 | 3.74 a* ± 0.45 | 4.37 ab* ± 0.45 | 5.32 * ± 0.65 | 4.34 * ± 0.65 | 4.57 * ± 0.65 | ||||||||
35–37 kDa group ψ | GW | 9.96 a ± 0.57 | 12.88 b ± 0.57 | 13.40 b ± 0.57 | 9.85 a ± 0.89 | 12.31 b ± 0.89 | 10.84 b ± 0.89 | *** | * | ** | ns | ns | ns | ns |
DW | 14.76 * ± 0.46 | 16.30 * ± 0.46 | 15.58 * ± 0.46 | 12.68 a#* ± 0.83 | 14.86 b* ± 0.83 | 15.10 b* ± 0.83 | ||||||||
26–30 kDa Group ϐ | GW | 11.91 a ± 0.48 | 13.20 ab ± 0.51 | 14.21 b ± 0.42 | 11.8 a ± 0.52 | 13.9 b ± 0.58 | 14.2 b ± 0.46 | *** | ns | *** | ns | ns | ns | ns |
DW | 13.10 a ± 0.48 | 16.12 b* ± 0.51 | 15.90 b* ± 0.42 | 12.3 a ± 0.52 | 15.7 b* ± 0.58 | 16.6 b* ± 0.46 |
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Martínez, T.F.; Alcalde, M.J.; Sáez, M.I.; Suárez, M.D. Effects of Farm Management Practices and Transport Time on Post-Mortem Changes of Longissimus lumborum Muscle Proteins in Suckling Goat Kids. Foods 2020, 9, 934. https://doi.org/10.3390/foods9070934
Martínez TF, Alcalde MJ, Sáez MI, Suárez MD. Effects of Farm Management Practices and Transport Time on Post-Mortem Changes of Longissimus lumborum Muscle Proteins in Suckling Goat Kids. Foods. 2020; 9(7):934. https://doi.org/10.3390/foods9070934
Chicago/Turabian StyleMartínez, Tomás Francisco, María Jesús Alcalde, María Isabel Sáez, and María Dolores Suárez. 2020. "Effects of Farm Management Practices and Transport Time on Post-Mortem Changes of Longissimus lumborum Muscle Proteins in Suckling Goat Kids" Foods 9, no. 7: 934. https://doi.org/10.3390/foods9070934