Systematic Myostatin Expression Screening Platform for Identification and Evaluation of Myogenesis-Related Phytogenic in Pigs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Plant Extract Preparation and Cells Transfection
2.3. MTT Assay
2.4. Luciferase Assays
2.5. Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
2.6. GUE Preparation
2.7. Animal Study
2.8. Pyrosequencing and Data Analysis
2.9. Statistics
3. Results
3.1. Establishment of Systematic Rodent Myoblast Screen Platform and Phytogenic Screening
3.2. Characterization and Identification of Active Fractions in GUE
3.3. Effect of GUE as a Feed Additive on Growth Performance in Growth-Finishing Pigs
3.4. Effect of GUE as a Feed Additive on Gut Microbiota in Growth-Finishing Pigs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment group | CTL | LGUE | MGUE | HGUE |
---|---|---|---|---|
Initial body weight (kg) | 11.71 ± 1.22 | 12.62 ± 1.62 | 12.89 ± 1.18 | 11.40 ± 1.18 |
Final body weight (kg) | 110.38 ± 6.29 a | 118.68 ± 8.12 b | 119.48 ± 6.54 b | 118.17 ± 10.91 b |
Body weight gain (kg) | 98.67 ± 7.38 a | 106.06 ± 7.48 b | 106.59 ± 6.43 b | 106.77 ± 9.83 b |
ADG (kg/day) | 0.78 ± 0.06 a | 0.84 ± 0.06 b | 0.85 ± 0.05 b | 0.85 ± 0.08 b |
ADFI (kg/day) | 2.00 ± 0.57 | 2.00 ± 0.42 | 2.00 ± 0.75 | 1.98 ± 0.82 |
TFI (kg) | 257.31 ± 7.91 | 256.92 ± 8.32 | 257.17 ± 6.11 | 255.16 ± 5.43 |
FCR | 2.61 a | 2.42 b | 2.41 b | 2.39 b |
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Ou, B.-R.; Hsu, M.-H.; Haung, L.-Y.; Lin, C.-J.; Kuo, L.-L.; Tsai, Y.-T.; Chang, Y.-C.; Lin, W.-Y.; Huang, T.-C.; Wu, Y.-C.; et al. Systematic Myostatin Expression Screening Platform for Identification and Evaluation of Myogenesis-Related Phytogenic in Pigs. Bioengineering 2023, 10, 1113. https://doi.org/10.3390/bioengineering10101113
Ou B-R, Hsu M-H, Haung L-Y, Lin C-J, Kuo L-L, Tsai Y-T, Chang Y-C, Lin W-Y, Huang T-C, Wu Y-C, et al. Systematic Myostatin Expression Screening Platform for Identification and Evaluation of Myogenesis-Related Phytogenic in Pigs. Bioengineering. 2023; 10(10):1113. https://doi.org/10.3390/bioengineering10101113
Chicago/Turabian StyleOu, Bor-Rung, Ming-Hua Hsu, Ling-Ya Haung, Chuan-Ju Lin, Li-Li Kuo, Yu-Ting Tsai, Yu-Chia Chang, Wen-Yuh Lin, Tsung-Chien Huang, Yun-Chu Wu, and et al. 2023. "Systematic Myostatin Expression Screening Platform for Identification and Evaluation of Myogenesis-Related Phytogenic in Pigs" Bioengineering 10, no. 10: 1113. https://doi.org/10.3390/bioengineering10101113