Green Synthesis and Antiproliferative Activity of Gold Nanoparticles of a Controlled Size and Shape Obtained Using Shock Wave Extracts from Amphipterygium adstringens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Obtaining Green Extracts
2.3. Optimization of Parameters for Green Extraction Methods
2.4. Antioxidant Capacity
2.5. Total Phenol and Flavonoid Content
2.6. Green Synthesis of AuNPs
2.7. AuNP Characterization
2.8. Viability Assay in Leukemia Cell Lines
2.8.1. Cell Culture
2.8.2. Cytotoxicity Assay with Trypan Blue
2.8.3. Statistics
3. Results and Discussion
3.1. Antioxidant Capacity
3.2. Green Synthesis of AuNPs
3.3. AuNP Characterization
3.4. Biological Assays
Viability Assay in Leukemia Cells Treated with AuNPs (Au@SWCD-EW and Au@US-W), SWCD-EW, and US-W Extracts
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | Acronym |
---|---|
A. adstringens methanolic macerate extraction | Mac-MeOH |
A. adstringens ethanol:water ultrasound-assisted extraction | US-EW |
A. adstringens ethanol:water shock wave-assisted extraction constant delay | SWCD-EW |
A. adstringens ethanol:water shock wave-assisted extraction variable delay | SWVD-EW |
A. adstringens water ultrasound-assisted extraction | US-W |
AuNPs synthesized in A. adstringens methanolic macerate extract | Au@Mac-MeOH |
AuNPs synthesized in A. adstringens ethanol:water ultrasound-assisted extraction | Au@US-EW |
AuNPs synthesized in A. adstringens water ultrasound-assisted extraction | Au@US-W |
AuNPs synthesized in A. adstringens ethanol:water shock wave-assisted extraction constant delay | Au@SWCD-EW |
AuNPs synthesized in A. adstringens ethanol:water shock wave-assisted extraction variable delay | Au@SWVD-EW |
Sample | TPC (mg/100 mLGAE) a | TFC (mg/100 mL QE) b | DPPH (mM/mL TEAC) c | CUPRAC (mM/mL TEAC) d |
---|---|---|---|---|
Mac-MeOH | 30.27 ± 0.25 | 60.06 ± 0.14 | 1359.91 ± 23.38 | 2765 ± 11.05 |
US-EW | 26.36 ± 0.43 | 75.41 ± 0.32 | 1836.72 ± 10.25 | 3018 ± 13.22 |
SWCD-EW | 53.31 ± 0.37 | 68.58 ±0.25 | 4838.95 ± 39.23 | 7051 ± 18.24 |
SWVD-EW | 39.15 ± 0.21 | 90.73 ± 0.18 | 2588.32 ± 50.17 | 5374 ± 36.44 |
US-W | 57.83 ± 0.32 | 97.96 ± 0.41 | 3537.69 ± 34.81 | 5109 ± 27.92 |
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Torres-Ortiz, D.; García-Alcocer, G.; Loske, A.M.; Fernández, F.; Becerra-Becerra, E.; Esparza, R.; Gonzalez-Reyna, M.A.; Estevez, M. Green Synthesis and Antiproliferative Activity of Gold Nanoparticles of a Controlled Size and Shape Obtained Using Shock Wave Extracts from Amphipterygium adstringens. Bioengineering 2023, 10, 437. https://doi.org/10.3390/bioengineering10040437
Torres-Ortiz D, García-Alcocer G, Loske AM, Fernández F, Becerra-Becerra E, Esparza R, Gonzalez-Reyna MA, Estevez M. Green Synthesis and Antiproliferative Activity of Gold Nanoparticles of a Controlled Size and Shape Obtained Using Shock Wave Extracts from Amphipterygium adstringens. Bioengineering. 2023; 10(4):437. https://doi.org/10.3390/bioengineering10040437
Chicago/Turabian StyleTorres-Ortiz, Daniela, Guadalupe García-Alcocer, Achim M. Loske, Francisco Fernández, Edgardo Becerra-Becerra, Rodrigo Esparza, Marlen Alexis Gonzalez-Reyna, and Miriam Estevez. 2023. "Green Synthesis and Antiproliferative Activity of Gold Nanoparticles of a Controlled Size and Shape Obtained Using Shock Wave Extracts from Amphipterygium adstringens" Bioengineering 10, no. 4: 437. https://doi.org/10.3390/bioengineering10040437