An Effective Chemical Permeabilization of Silkworm Embryos
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection of Eggs and Embryonic Stages
2.2. Dechorionation and Permeabilization Processes
2.3. Rhodamine B Staining
2.4. Osmotic Response of Embryos to Sucrose and Ethylene Glycol (EG), and Data Analysis
2.5. EG Treatment in Viability Assay
2.6. Assessment of Development and Viability
2.7. Statistical Analysis
3. Results
3.1. Improvements in the Permeabilization Process
3.2. Permeability at Different Embryonic Stages
3.3. Osmotic Response of Embryos to Sucrose
3.4. More Detailed Analysis of the Osmotic Response during Embryonic Developmental Stages
3.5. Viability of Embryos after Permeabilization
3.6. Effect of Hexane Exposure Time
3.7. Effect of Permeabilization with Chemicals Other Than Hexane
3.8. Effect on Different Strains: Diapausing
3.9. Osmotic Response of Embryos to EG and Viability
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Urbán-Duarte, D.; Tomita, S.; Sakai, H.; Sezutsu, H.; De La Torre-Sánchez, J.F.; Kainoh, Y.; Furukawa, S.; Uchino, K. An Effective Chemical Permeabilization of Silkworm Embryos. Bioengineering 2023, 10, 563. https://doi.org/10.3390/bioengineering10050563
Urbán-Duarte D, Tomita S, Sakai H, Sezutsu H, De La Torre-Sánchez JF, Kainoh Y, Furukawa S, Uchino K. An Effective Chemical Permeabilization of Silkworm Embryos. Bioengineering. 2023; 10(5):563. https://doi.org/10.3390/bioengineering10050563
Chicago/Turabian StyleUrbán-Duarte, David, Shuichiro Tomita, Hiroki Sakai, Hideki Sezutsu, José Fernando De La Torre-Sánchez, Yooichi Kainoh, Seiichi Furukawa, and Keiro Uchino. 2023. "An Effective Chemical Permeabilization of Silkworm Embryos" Bioengineering 10, no. 5: 563. https://doi.org/10.3390/bioengineering10050563