Functional Blockage of S100A8/A9 Ameliorates Ischemia–Reperfusion Injury in the Lung
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Lung IR Model and Reagents
2.3. Histological and Immunohistochemistry Staining
2.4. ELISA
2.5. RNA Isolation and Sequencing
2.6. Quantitative Real-Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
2.7. Western Blotting
2.8. Apoptosis Analysis
2.9. Statistical Analysis
3. Results
3.1. S100A8/A9 as an Early-Response Gene of IR Injury in the Lung
3.2. S100A8/A9 Production after IR Injury and Inhibition with Neutral Antibody
3.3. S100A8/A9 Localization in IR Injury
3.4. Improvement in Lung Function Because of S100A8/A9 Inhibition
3.5. Anti-S100A8/A9 mAb Reduces Lung Injury, S100A9-Positive Cells, and Neutrophil Infiltration
3.6. Anti-S100A8/A9 mAb Suppresses Proinflammatory Cytokines and Chemokines
3.7. Anti-S100A8/A9 mAb Ameliorated MAPK Signaling
3.8. Anti-S100A8/A9 mAb Prevents Apoptosis in the Lung
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nakata, K.; Okazaki, M.; Sakaue, T.; Kinoshita, R.; Komoda, Y.; Shimizu, D.; Yamamoto, H.; Tanaka, S.; Suzawa, K.; Shien, K.; et al. Functional Blockage of S100A8/A9 Ameliorates Ischemia–Reperfusion Injury in the Lung. Bioengineering 2022, 9, 673. https://doi.org/10.3390/bioengineering9110673
Nakata K, Okazaki M, Sakaue T, Kinoshita R, Komoda Y, Shimizu D, Yamamoto H, Tanaka S, Suzawa K, Shien K, et al. Functional Blockage of S100A8/A9 Ameliorates Ischemia–Reperfusion Injury in the Lung. Bioengineering. 2022; 9(11):673. https://doi.org/10.3390/bioengineering9110673
Chicago/Turabian StyleNakata, Kentaro, Mikio Okazaki, Tomohisa Sakaue, Rie Kinoshita, Yuhei Komoda, Dai Shimizu, Haruchika Yamamoto, Shin Tanaka, Ken Suzawa, Kazuhiko Shien, and et al. 2022. "Functional Blockage of S100A8/A9 Ameliorates Ischemia–Reperfusion Injury in the Lung" Bioengineering 9, no. 11: 673. https://doi.org/10.3390/bioengineering9110673