Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma

Shtykalova, Sofia; Egorova, Anna; Maretina, Marianna; Baranov, Vladislav; Kiselev, Anton

doi:10.3390/bioengineering9030112

Open AccessArticle

Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma

by

Sofia Shtykalova

,

Anna Egorova

,

Marianna Maretina

,

Vladislav Baranov

and

Anton Kiselev

^*

Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya Line 3, 199034 Saint-Petersburg, Russia

^*

Author to whom correspondence should be addressed.

Bioengineering 2022, 9(3), 112; https://doi.org/10.3390/bioengineering9030112

Submission received: 29 January 2022 / Revised: 3 March 2022 / Accepted: 6 March 2022 / Published: 8 March 2022

(This article belongs to the Special Issue Novel Expansions and Trends in Gene Diagnostics and Gene Therapy)

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Abstract

Suicidegene therapy is considered a promising approach for the treatment of uterine leiomyoma (UL), a benign tumor in women characterized by precise localization. In this study, we investigate the efficiency of αvβ3 integrin-targeted arginine-rich peptide carrier R6p-cRGD electrostatically bound to magnetic nanoparticles (MNPs) for targeted DNA delivery into the UL cells. The physico–chemical and cytotoxic properties, transfection efficiency, and specificity of R6p-cRGD/DNA/MNPs polyplexes were evaluated. The addition of MNPs resulted in a decrease in the time needed for successful transfection with simultaneous increase in efficiency. We revealed a therapeutic effect on primary UL cells after delivery of plasmid encoding the herpes simplex virus type 1 (HSV-1) thymidine kinase gene. Treatment with ganciclovir resulted in 20% efficiency of suicide gene therapy in UL cells transfected with the pPTK-1 plasmid. Based on these results, we conclude that the use of cationic peptide carriers with MNPs can be promising for the development of modular non-viral carriers for suicide gene delivery to UL cells.

Keywords: DNA delivery; peptide-based carriers; gene therapy; magnetic nanoparticles; thymidine kinase; uterine leiomyoma; integrins

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MDPI and ACS Style

Shtykalova, S.; Egorova, A.; Maretina, M.; Baranov, V.; Kiselev, A. Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma. Bioengineering 2022, 9, 112. https://doi.org/10.3390/bioengineering9030112

AMA Style

Shtykalova S, Egorova A, Maretina M, Baranov V, Kiselev A. Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma. Bioengineering. 2022; 9(3):112. https://doi.org/10.3390/bioengineering9030112

Chicago/Turabian Style

Shtykalova, Sofia, Anna Egorova, Marianna Maretina, Vladislav Baranov, and Anton Kiselev. 2022. "Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma" Bioengineering 9, no. 3: 112. https://doi.org/10.3390/bioengineering9030112

APA Style

Shtykalova, S., Egorova, A., Maretina, M., Baranov, V., & Kiselev, A. (2022). Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma. Bioengineering, 9(3), 112. https://doi.org/10.3390/bioengineering9030112

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Magnetic Nanoparticles as a Component of Peptide-Based DNA Delivery System for Suicide Gene Therapy of Uterine Leiomyoma

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