Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. miRNAs, Plasmids and Target Prediction
2.3. Triglyceride (TG) Level Determination
2.4. Transcriptomic Analysis
2.5. Quantitative Real-Time PCR
2.6. Western Blot
2.7. Luciferase Reporter Assay
2.8. Statistical Analysis
2.9. Data Available
3. Results
3.1. Let-7f-5p Restricts PRRSV Replication and Regulates Lipid Metabolism
3.2. Let-7f-5p Altered Lipogenesis Pathway in PRRSV-Infected PK-15CD163 Cells
3.3. qRT-PCR Verified That Let-7f-5p Targeted Lipid Metabolism Pathway
3.4. Let-7f-5p Directly Targets SREBP2 to Modulate Lipid Metabolism
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jiang, D.; Yang, L.; Meng, X.; Xu, Q.; Zhou, X.; Liu, B. Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection. Vet. Sci. 2024, 11, 392. https://doi.org/10.3390/vetsci11090392
Jiang D, Yang L, Meng X, Xu Q, Zhou X, Liu B. Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection. Veterinary Sciences. 2024; 11(9):392. https://doi.org/10.3390/vetsci11090392
Chicago/Turabian StyleJiang, Dongfeng, Liyu Yang, Xiangge Meng, Qiuliang Xu, Xiang Zhou, and Bang Liu. 2024. "Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection" Veterinary Sciences 11, no. 9: 392. https://doi.org/10.3390/vetsci11090392