The Effects of Colchicum Dispert and Bone Marrow-Derived Mesenchymal Stem Cell Therapy on Skeletal Muscle Injury in a Rat Aortic Ischemia–Reperfusion Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethical Approval and Animal Subjects
2.2. Experimental Design
2.3. Ischemia–Reperfusion Injury Model
2.3.1. Mesenchymal Stem Cell Preparation
2.3.2. Surface Antigen Characterization
2.4. Blood Sample Analysis
2.5. Tissue Sample Analysis
2.6. Statistical Analysis
3. Results
3.1. Biochemical Results
3.2. Skeletal Muscle Tissue Results
3.3. Apoptosis Results in Skeletal Muscle
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Weight Measurement | n | Pre-Study (g) (Mean ± SD) | Study (g) (Mean ± SD) |
---|---|---|---|
Control | 6 | 618.67 ± 39.79 | 614.33 ± 40.51 |
Sham | 7 | 596.86 ± 53.73 | 604.57 ± 59.37 |
IRI | 8 | 612.75 ± 41.53 | 612.25 ± 45.57 |
COL | 6 | 602.67 ± 39.87 | 545.00 ± 44.81 |
MSC | 8 | 598.00 ± 42.28 | 601.50 ± 50.24 |
Mix | 7 | 596.00 ± 33.86 | 586.43 ± 49.95 |
p-Value | 0.842 | 0.142 |
CONTROL (n = 6) | SHAM (n = 7) | IRI (n = 8) | COL (n = 6) | MSC (n = 8) | MIX (n = 7) | p-Value | |
---|---|---|---|---|---|---|---|
Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | ||
TAC | 1.47 ± 0.09 | 1.65 ± 0.09 | 1.45 ± 0.11 | 1.34 ± 0.09 | 1.38 ± 0.11 | 1.24 ± 0.12 | 0.52 |
TOS | 6.72 ± 1.23 | 6.24 ± 2.91 | 11.17 ± 7.17 | 9.11 ± 3.96 | 9.31 ± 6.07 | 8.28 ± 4.26 | 0.039 * |
OSI | 4.55 ± 0.65 | 3.77 ± 1.72 | 7.61 ± 4.50 | 6.80 ± 2.90 | 6.78 ± 4.46 | 6.92 ± 4.15 | 0.000 * |
NT (mmol/L) | 159.67 ± 28.93 | 211.14 ± 111.69 | 254.5 ± 85.50 | 297.83 ± 79,71 | 289.63 ± 127.91 | 279.86 ± 124.6 | 0.055 |
TT (mmol/L) | 288.5 ± 41.38 | 374.29 ± 211.54 | 475.75 ± 193.39 | 464.17 ± 169.36 | 560.75 ± 201.25 | 502.86 ± 183.84 | 0.154 |
DYSS (mmol/L) | 64.42 ± 21.62 | 81.57 ± 55.53 | 110.63 ± 58.76 | 83.17 ± 46.82 | 135.56 ± 73.29 | 111.5 ± 59.32 | 0.337 |
DYSS/TT (%) | 22.01 ± 5.64 | 21.69 ± 7.00 | 22.15 ± 4.42 | 16.67 ± 4.79 | 24.52 ± 9.66 | 22.84 ± 9.54 | 0.52 |
DYSS/NT (%) | 43.00 ± 22.10 | 44.02 ± 27.98 | 42.00 ± 16.32 | 26.24 ± 10.29 | 61.96 ± 43.8 | 56.23 ± 49.77 | 0.52 |
NT/TT (%) | 55.97 ± 11.28 | 56.63 ± 13.99 | 55.70 ± 8.85 | 66.66 ± 9.57 | 50.96 ± 19.32 | 54.32 ± 19.08 | 0.261 |
Control (n = 6) | Sham (n = 7) | IRI (n = 8) | COL (n = 6) | MSC (n = 8) | Mix (n = 7) | p-Value | |
---|---|---|---|---|---|---|---|
Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | ||
IMA | 1.43 ± 0.28 | 1.68 ± 0.12 | 1.66 ± 0.11 | 1.69 ± 0.11 | 1.65 ± 0.23 | 1.29 ± 0.31 | 0.020 * |
ADMA | 0.21 ± 0.15 | 0.16 ± 0.09 | 0.26 ± 0.09 | 0.41 ± 0.36 | 0.23 ± 0.09 | 0.3 ± 0.09 | 0.069 |
SDMA | 0.40 ± 0.26 | 0.34 ± 0.09 | 0.43 ± 0.10 | 0.61 ± 0.42 | 0.40 ± 0.15 | 0.27 ± 0.08 | 0.097 |
L-NMMA | 0.08 ± 0.06 | 0.07 ± 0.05 | 0.07 ± 0.04 | 0.10 ± 0.10 | 0.06 ± 0.04 | 0.06 ± 0.03 | 1.00 |
ARG | 69.66 ± 13.77 | 54.87 ± 11.88 | 34.23 ± 20.88 | 67.53 ± 22.78 | 47.35 ± 20.2 | 58.42 ± 18.78 | 0.028 * |
CIT | 133.45 ± 67.03 | 131.79 ± 66.71 | 206.00 ± 78.48 | 112.15 ± 60.27 | 147.48 ± 63.4 | 103.13 ± 35.31 | 0.132 |
ORN | 28.25 ± 15.16 | 21.35 ± 10.12 | 30.05 ± 7.28 | 21.22 ± 11.56 | 27.85 ± 11.16 | 20.51 ± 5.74 | 0.336 |
HoARG | 1.42 ± 0.80 | 1.09 ± 0.39 | 1.64 ± 0.59 | 1.52 ± 0.74 | 1.36 ± 0.98 | 1.08 ± 0.49 | 0.479 |
CONTROL (n = 6) | SHAM (n = 7) | IRI (n = 8) | COL (n = 6) | MSC (n = 8) | MIX (n = 7) | p-Value | |
---|---|---|---|---|---|---|---|
Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | ||
TAC | 1.01 ± 0.08 | 0.98 ± 0.07 | 0.98 ± 0.12 | 0.99 ± 0.14 | 0.83 ± 0.07 | 0.96 ± 0.02 | 0.020 * |
TOS | 9.86 ± 3.80 | 9.87 ± 3.07 | 11.67 ± 2.86 | 14.63 ± 10.59 | 9.74 ± 2.51 | 11.11 ± 0.01 | 0.577 |
OSI | 9.74 ± 3.49 | 9.94 ± 2.58 | 11.76 ± 2.06 | 14.09 ± 7.60 | 11.68 ± 2.3 | 11.42 ± 0.01 | 0.499 |
ADMA | 0.20 ± 0.10 | 0.18 ± 0.09 | 0.23 ± 0.07 | 0.38 ± 0.37 | 0.24 ± 0.06 | 0.39 ± 0.19 | 0.093 |
SDMA | 0.10 ± 0.06 | 0.11 ± 0.03 | 0.12 ± 0.04 | 0.23 ± 0.17 | 0.07 ± 0.02 | 0.10 ± 0.02 | 0.017 * |
L-NMMA | 0.03 ± 0.01 | 0.03 ± 0.02 | 0.04 ± 0.03 | 0.04 ± 0.02 | 0.03 ± 0.01 | 0.07 ± 0.05 | 0.417 |
ARG | 34.52 ± 13.31 | 49.27 ± 16.08 | 38.65 ± 17.80 | 41.95 ± 14.66 | 26.21 ± 11.21 | 42.54 ± 14.43 | 0.108 |
CIT | 17.53 ± 11.88 | 19.41 ± 8.11 | 22.73 ± 17.69 | 13.70 ± 8.58 | 13.65 ± 3.97 | 37.27 ± 16.66 | 0.036 * |
ORN | 3.10 ± 1.60 | 3.54 ± 1.86 | 3.83 ± 1.52 | 3.58 ± 0.81 | 3.93 ± 1 | 5.19 ± 1.79 | 0.29 |
HOARG | 0.20 ± 0.09 | 0.26 ± 0.19 | 0.32 ± 0.51 | 0.19 ± 0.08 | 0.25 ± 0.32 | 1.23 ± 1.51 | 0.092 |
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Orhan, A.; Çiçek, Ö.F.; Öztürk, B.; Akbayrak, H.; Ünlükal, N.; Vatansev, H.; Solmaz, M.; Büyükateş, M.; Aniç, S.; Ovalı, F.; et al. The Effects of Colchicum Dispert and Bone Marrow-Derived Mesenchymal Stem Cell Therapy on Skeletal Muscle Injury in a Rat Aortic Ischemia–Reperfusion Model. J. Cardiovasc. Dev. Dis. 2024, 11, 251. https://doi.org/10.3390/jcdd11080251
Orhan A, Çiçek ÖF, Öztürk B, Akbayrak H, Ünlükal N, Vatansev H, Solmaz M, Büyükateş M, Aniç S, Ovalı F, et al. The Effects of Colchicum Dispert and Bone Marrow-Derived Mesenchymal Stem Cell Therapy on Skeletal Muscle Injury in a Rat Aortic Ischemia–Reperfusion Model. Journal of Cardiovascular Development and Disease. 2024; 11(8):251. https://doi.org/10.3390/jcdd11080251
Chicago/Turabian StyleOrhan, Atilla, Ömer Faruk Çiçek, Bahadır Öztürk, Hakan Akbayrak, Nejat Ünlükal, Hakan Vatansev, Merve Solmaz, Mustafa Büyükateş, Seda Aniç, Fadime Ovalı, and et al. 2024. "The Effects of Colchicum Dispert and Bone Marrow-Derived Mesenchymal Stem Cell Therapy on Skeletal Muscle Injury in a Rat Aortic Ischemia–Reperfusion Model" Journal of Cardiovascular Development and Disease 11, no. 8: 251. https://doi.org/10.3390/jcdd11080251