Identification of Growth-Promoting Bacterial Resources by Investigating the Microbial Community Composition of Polyporus umbellatus Sclerotia
Abstract
:1. Introduction
2. Materials and Methods
2.1. P. umbellatus Cultivation and Harvest
2.2. Collection of Soil Samples
2.3. Ergosterol Content Determination
2.4. Polysaccharides Content Determination
2.5. DNA Extraction and 16S rRNA/ITS Gene Sequencing
2.6. Diversity of Microbial Communities Associated with P. umbellatus
2.7. Isolation and Identification of Microbial Communities Associated with P. umbellatus
2.8. Co-Culture Experiments with P. umbellatus Mycelium
2.9. Production of IAA
2.10. Phosphate Solubilization
2.11. Siderophore Production
2.12. Nitrogen Fixation
2.13. Antagonism Assays
2.14. Bacterial Consortium Inoculants
2.15. Statistical Analysis
3. Results
3.1. Yield, Ergosterol, and Polysaccharides Content in P. umbellatus
3.2. Microbial Communities in the Soil of the P. umbellatus Habitat
3.3. Microbial Isolation, Identification, and Effects on Mycelial Growth
3.4. Plant Growth-Promoting (PGP) Traits of Bacterial Isolates
3.5. Promotion of P. umbellatus Mycelial Growth by Multiple Isolates
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, T.; Cheng, R.; Hua, Z.; Gao, H.; Wang, C.; Li, H.; Yuan, Y. Identification of Growth-Promoting Bacterial Resources by Investigating the Microbial Community Composition of Polyporus umbellatus Sclerotia. J. Fungi 2024, 10, 386. https://doi.org/10.3390/jof10060386
Liu T, Cheng R, Hua Z, Gao H, Wang C, Li H, Yuan Y. Identification of Growth-Promoting Bacterial Resources by Investigating the Microbial Community Composition of Polyporus umbellatus Sclerotia. Journal of Fungi. 2024; 10(6):386. https://doi.org/10.3390/jof10060386
Chicago/Turabian StyleLiu, Tianrui, Rui Cheng, Zhongyi Hua, Haiyun Gao, Chu Wang, Hui Li, and Yuan Yuan. 2024. "Identification of Growth-Promoting Bacterial Resources by Investigating the Microbial Community Composition of Polyporus umbellatus Sclerotia" Journal of Fungi 10, no. 6: 386. https://doi.org/10.3390/jof10060386