Asperinsuterpenes A–C from the Fungus Aspergillus insuetus BTBU20220155
Abstract
:1. Introduction
2. Materials and Methods
2.1. Molecular Identification
2.2. General Experimental Procedure
2.3. Fungal Materials, Cultivation, Fermentation, and Isolation
2.4. ECD Calculation Methods
2.5. Antimicrobial Assay
3. Results and Discussion
3.1. Phylogenetic Analysis
3.2. Structure Elucidation
3.3. Antimicrobial Activities of the Isolated Compounds
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Position | 1 | 2 | ||
---|---|---|---|---|
δC | δH, (J in Hz) | δC | δH, (J in Hz) | |
1 | 148.5, CH | 6.32, d (13.0) | 35.2, CH2 | 2.07, m; 2.00, m |
2 | 121.7, CH | 5.90, d (13.0) | 30.3, CH2 | 2.44, m |
3 | 166.0, C | 174.6, C | ||
4 | 84.3, CH | 74.8, C | ||
5 | 51.5, CH | 2.16, dd (8.5, 6.5) | 50.7, CH | 1.73, m |
6α | 21.2, CH2 | 1.83, m | 23.1, CH2 | 1.88, m |
6β | 1.66, m | |||
7α | 29.9, CH2 | 1.91, m | 29.6, CH2 | 1.70, m |
7β | 1.73, m | 1.60, m | ||
8 | 39.0, CH | 2.12, m | 37.4, CH | 2.03, m |
9 | 92.8, C | 96.7, C | ||
10 | 51.5, C | 46.4, C | ||
11α | 44.5, CH2 | 3.31, dd (18.0, 2.0) | 40.4, CH2 | 3.62, dd (19.0, 2.0) |
11β | 3.26, dd (18.0, 1.5) | 3.20, dd (19.0, 2.0) | ||
12 | 141.9, C | 143.1, C | ||
13 | 131.2, CH | 6.87, brs | 130.0, CH | 6.91, s |
14 | 139.8, C | 139.6, C | ||
15 | 143.6, C | 143.4, C | ||
16 | 181.3, C | 181.3, C | ||
17 | 127.8, C | 126.3, C | ||
18 | 163.5, C | 165.1, C | ||
19 | 23.9, CH3 | 2.14, s | 23.9, CH3 | 2.15, s |
20 | 169.2, C | 169.2, C | ||
21 | 52.0, OCH3 | 3.78, s | 51.9, OCH3 | 3.76, s |
22 | 13.3, CH3 | 2.13, s | 13.7, CH3 | 2.09, s |
23 | 18.0, CH3 | 1.57, s | 18.7, CH3 | 1.25, s |
24 | 16.9, CH3 | 1.22, d (7.5) | 16.7, CH3 | 0.88, d (7.0) |
25α | 32.1, CH3 | 1.41, s | 28.9, CH3 | 1.31, s |
26β | 25.6, CH3 | 1.50, s | 34.5, CH3 | 1.34, s |
27 | 51.6, CH3 | 3.59, s | ||
4-OH | 3.45, s |
Position | 3 | |
---|---|---|
δC | δH, (J in Hz) | |
1a | 33.2, CH2 | 1.91, m |
1b | 1.82, m | |
2a | 32.8, CH2 | 2.74, m |
2b | 2.68, ddd (15.0, 7.5, 4.0) | |
3 | 173.9, C | |
4 | 85.1, C | |
5 | 50.0, CH | 2.07, m |
6α | 22.3, CH2 | 1.84, m |
6β | 1.55, m, | |
7α | 29.9, CH2 | 1.83, m |
7β | 1.51, m | |
8 | 38.6, CH | 1.85, m |
9 | 100.7, C | |
10 | 44.4, C | |
11α | 37.5, CH2 | 2.35, d (13.5) |
11β | 2.23, d (13.5) | |
12 | 48.8, C | |
13 | 156.1, C | |
14 | 132.8, C | |
15 | 184.2, C | |
16 | 108.8, C | |
17 | 179.8, C | |
18 | 35.0, CH3 | 1.60, s |
19 | 16.8, CH3 | 2.05, s |
20 | 168.1, C | |
21 | 52.0, OCH3 | 3.74, s |
22 | 8.1, CH3 | 1.75, s |
23 | 17.5, CH3 | 1.39, s |
24 | 16.6, CH3 | 0.94, d (6.5) |
25 | 32.3, CH3 | 1.45, s |
26 | 25.1, CH3 | 1.42, s |
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Zhang, X.; Song, F.; Han, J.; Wang, L.; Ma, L.; Xu, X. Asperinsuterpenes A–C from the Fungus Aspergillus insuetus BTBU20220155. J. Fungi 2024, 10, 611. https://doi.org/10.3390/jof10090611
Zhang X, Song F, Han J, Wang L, Ma L, Xu X. Asperinsuterpenes A–C from the Fungus Aspergillus insuetus BTBU20220155. Journal of Fungi. 2024; 10(9):611. https://doi.org/10.3390/jof10090611
Chicago/Turabian StyleZhang, Xinjun, Fuhang Song, Jiahui Han, Long Wang, Linlin Ma, and Xiuli Xu. 2024. "Asperinsuterpenes A–C from the Fungus Aspergillus insuetus BTBU20220155" Journal of Fungi 10, no. 9: 611. https://doi.org/10.3390/jof10090611