Metarhizium caribense sp. nov., a Novel Species of Entomopathogenic Metarhizium Fungi Associated with Weevils Impairing Coffee, Sugar Cane and Sweet Potato Cultivation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains and Isolates
2.2. Morphological and Cultural Characterization of Fungal Isolates
2.3. DNA Extraction
2.4. Preparative PCR Amplification and Marker Sequence Determination
2.5. Phylogenetic Reconstruction
2.6. Diagnostic PCR Amplification for Species Discrimination
3. Results
3.1. Molecular Taxonomic Identification Based on Protein-Encoding Gene Sequences
3.2. Molecular Taxonomic Identification Based on Intergenic Spacer (IGS) Sequences
3.3. rIGS-Based Species-Discriminating Diagnostic PCR
3.4. 28S Ribosomal RNA Gene Group-I Intron Analysis
3.5. Morphological and Cultural Characterization of Fungal Isolates
4. Taxonomic Description
5. Discussion
6. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain Designation | Source of Isolation | Geographic Origin |
---|---|---|
LBM-30 | Soil using Galleria mellonella (L.) (Lepidoptera: Pyralidae) bait method | La Habana, Cuba |
LBM-41 | Coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Curculionidae) | Granma, Cuba |
LBM-42 | Coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Curculionidae) | Granma, Cuba |
Isolate | Colony Characteristics | Conidial Size a | Conidial Shape |
---|---|---|---|
LBM-30 | Cottony texture, abundant mycelia growth and wooly in the central area, with a white mycelial margin. Abundant conidiation yellow to olive green in color and crusty appearance, with regular border. Conidiophores broadly branched (chandelier-like) | 5.00–8.50 (6.60) × 2.00–3.00 (2.44) | Cylindrical |
LBM-41 | White colonies to greenish olivaceous, forming halos or concentric rings with conidia maturation. Abundant conidiation in central area forming small pustules, with white mycelial margin. Ochreous pigment diffusing into medium. Conidiophores broadly branched (chandelier-like). | 4.88–7.32 (5.66) × 2.44–3.66 (2.71). | Cylindrical to ellipsoidal |
LBM-42 | White to dark olivaceous green with conidia maturation, colonies with white mycelial margin. Diffuse conidiation forming small pustules. Ochreous pigment diffusing into medium | 4.88–7.32 (6.93) × 2.43–2.44 (2.44). | Cylindrical to ellipsoidal |
ARSEF 1448 | Colonies were white and became yellow to olive green with a white mycelial margin. Colony reverses were yellow. Conidiophores were broadly branched, like a candelabrum arrangement. Phialides were cylindrical and organized in compact palisades. | 4.91–7.19 (5.99) × 1.29–2.56 (1.83). | Cylindrical |
ARSEF 5197 | Colonies initially appeared white and became yellow to dark olive green with white mycelial margin. Colony reverses were ochreous. Conidiophores were broadly branched, like a candelabrum arrangement. Phialides were cylindrical and organized in compact palisades | 5.08–7.04 (5.78) × 1.22–2.39 (1.82) | Cylindrical |
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Baró Robaina, Y.; Schuster, C.; Castañeda-Ruiz, R.F.; Gato Cárdenas, Y.; Márquez Gutiérrez, M.E.; Ponce de la Cal, A.; Leclerque, A. Metarhizium caribense sp. nov., a Novel Species of Entomopathogenic Metarhizium Fungi Associated with Weevils Impairing Coffee, Sugar Cane and Sweet Potato Cultivation. J. Fungi 2024, 10, 612. https://doi.org/10.3390/jof10090612
Baró Robaina Y, Schuster C, Castañeda-Ruiz RF, Gato Cárdenas Y, Márquez Gutiérrez ME, Ponce de la Cal A, Leclerque A. Metarhizium caribense sp. nov., a Novel Species of Entomopathogenic Metarhizium Fungi Associated with Weevils Impairing Coffee, Sugar Cane and Sweet Potato Cultivation. Journal of Fungi. 2024; 10(9):612. https://doi.org/10.3390/jof10090612
Chicago/Turabian StyleBaró Robaina, Yamilé, Christina Schuster, Rafael F. Castañeda-Ruiz, Yohana Gato Cárdenas, María Elena Márquez Gutiérrez, Amaia Ponce de la Cal, and Andreas Leclerque. 2024. "Metarhizium caribense sp. nov., a Novel Species of Entomopathogenic Metarhizium Fungi Associated with Weevils Impairing Coffee, Sugar Cane and Sweet Potato Cultivation" Journal of Fungi 10, no. 9: 612. https://doi.org/10.3390/jof10090612