Rad6 Regulates Conidiation by Affecting the Biotin Metabolism in Beauveria bassiana
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bioinformatic Analysis of Fungal Rad6 Homologs
2.2. Generation of rad6 Mutants
2.3. Cultivation Conditions
2.4. Hyphal Biomass and Conidial Production
2.5. Transcriptome Analysis and Verification
2.6. Phenotype Validation Experiments
2.7. Statistical Analysis
3. Results
3.1. Bioinformatical Analysis of Rad6 Protein in B. bassiana
3.2. Vital Role of Rad6 Protein in Conidiation and Hyphal Growth
3.3. Transcriptomic View of Rad6-Mediated Regulation Pathway Differences
3.4. Conidiation Increases with Biotin Addition
3.5. Transcription Pattern of CDP Genes in Different Media with and without Exogenous Biotin
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Guo, Y.; He, H.; Guan, Y.; Zhang, L. Rad6 Regulates Conidiation by Affecting the Biotin Metabolism in Beauveria bassiana. J. Fungi 2024, 10, 613. https://doi.org/10.3390/jof10090613
Guo Y, He H, Guan Y, Zhang L. Rad6 Regulates Conidiation by Affecting the Biotin Metabolism in Beauveria bassiana. Journal of Fungi. 2024; 10(9):613. https://doi.org/10.3390/jof10090613
Chicago/Turabian StyleGuo, Yuhan, Haomin He, Yi Guan, and Longbin Zhang. 2024. "Rad6 Regulates Conidiation by Affecting the Biotin Metabolism in Beauveria bassiana" Journal of Fungi 10, no. 9: 613. https://doi.org/10.3390/jof10090613