The Cryptochrome CryA Regulates Lipid Droplet Accumulation, Conidiation, and Trap Formation via Responses to Light in Arthrobotrys oligospora
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Bioinformatic Analysis
2.3. The AocryA Knockout
2.4. Determination of Mycelial Growth, Spore Yield, and Germination Rate
2.5. Observation of Mycelial Septa, Nuclei, Lipid Droplets (LDs), and Endocytosis
2.6. Analysis of Trap Formation, Pathogenicity, and Proteolytic Activity
2.7. Stress Tolerance Analysis
2.8. RT-PCR Analysis
2.9. Secondary Metabolic Analysis
2.10. Data Analysis
3. Results
3.1. Bioinformatics Analysis of AoCryA and Verification of Deletion Strains
3.2. AoCryA Is Involved in Sporulation by Regulating the Response of Light
3.3. AoCryA Affects the Length of Hyphal Cells and the Number of Nuclei
3.4. AoCryA Negatively Regulates Trap Formation and Pathogenicity
3.5. AoCryA Regulates Stress Response and Carotenoid Synthesis
3.6. AoCryA Regulates LD Accumulation and Endocytosis
3.7. AoCryA Regulates Secondary Metabolites
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shen, Y.; Yang, X.; Zhu, M.; Duan, S.; Liu, Q.; Yang, J. The Cryptochrome CryA Regulates Lipid Droplet Accumulation, Conidiation, and Trap Formation via Responses to Light in Arthrobotrys oligospora. J. Fungi 2024, 10, 626. https://doi.org/10.3390/jof10090626
Shen Y, Yang X, Zhu M, Duan S, Liu Q, Yang J. The Cryptochrome CryA Regulates Lipid Droplet Accumulation, Conidiation, and Trap Formation via Responses to Light in Arthrobotrys oligospora. Journal of Fungi. 2024; 10(9):626. https://doi.org/10.3390/jof10090626
Chicago/Turabian StyleShen, Yanmei, Xuewei Yang, Meichen Zhu, Shipeng Duan, Qianqian Liu, and Jinkui Yang. 2024. "The Cryptochrome CryA Regulates Lipid Droplet Accumulation, Conidiation, and Trap Formation via Responses to Light in Arthrobotrys oligospora" Journal of Fungi 10, no. 9: 626. https://doi.org/10.3390/jof10090626