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10.3390/jof11010016
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Article

Establishment of a Mutant Library for Infection Cushion Development and Identification of a Key Regulatory Gene in Botrytis cinerea

by
Maoyao Tang
1,
Kexin Wang
1,
Pan Zhang
2,
Jie Hou
2,
Xiaoqian Yu
1,
Hongfu Wang
1,
Yangyizhou Wang
1 and
Guihua Li
1,*
1
College of Plant Sciences, Jilin University, Changchun 130062, China
2
Engineering Research Centre of Forestry Biotechnology, Jilin Province in Beihua University, Jilin 132013, China
*
Author to whom correspondence should be addressed.
J. Fungi 2025, 11(1), 16; https://doi.org/10.3390/jof11010016
Submission received: 28 November 2024 / Revised: 20 December 2024 / Accepted: 27 December 2024 / Published: 29 December 2024
(This article belongs to the Special Issue Biodiversity, Systematics, and Evolution of Plant Pathogenic Fungi)
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Abstract

Botrytis cinerea, the grey mould fungus affecting over 1400 plant species, employs infection cushion (IC), a branched and claw-like structure formed by mycelia, as a critical strategy to breach host surface barriers. However, the molecular mechanisms underlying IC formation remain largely unexplored. In this study, we utilized a forward genetics approach to establish a large T-DNA tagged population of B. cinerea, which contained 14,000 transformants. Through phenotype screening, we identified 161 mutants with defects in IC development. Detailed analyses revealed that these mutants exhibited various degrees of impairment in IC formation, ranging from complete failure to form ICs to a reduction in the number and maturity of ICs. Further genetic analysis of one of the mutants led to the identification of EXO70, a gene encoding a component of the exocyst complex, as a key regulatory factor in IC development. Mutants with deletion of EXO70 failed to form ICs, confirming its crucial role in the process. The mutant library reported here provides a rich resource for further large-scale identification of genes involved in IC development. Our findings provide valuable insights into the genetic and molecular basis of IC formation and offer new targets for controlling B. cinerea pathogenicity.
Keywords: forward genetics; mutant library; ATMT; EXO70 forward genetics; mutant library; ATMT; EXO70

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MDPI and ACS Style

Tang, M.; Wang, K.; Zhang, P.; Hou, J.; Yu, X.; Wang, H.; Wang, Y.; Li, G. Establishment of a Mutant Library for Infection Cushion Development and Identification of a Key Regulatory Gene in Botrytis cinerea. J. Fungi 2025, 11, 16. https://doi.org/10.3390/jof11010016

AMA Style

Tang M, Wang K, Zhang P, Hou J, Yu X, Wang H, Wang Y, Li G. Establishment of a Mutant Library for Infection Cushion Development and Identification of a Key Regulatory Gene in Botrytis cinerea. Journal of Fungi. 2025; 11(1):16. https://doi.org/10.3390/jof11010016

Chicago/Turabian Style

Tang, Maoyao, Kexin Wang, Pan Zhang, Jie Hou, Xiaoqian Yu, Hongfu Wang, Yangyizhou Wang, and Guihua Li. 2025. "Establishment of a Mutant Library for Infection Cushion Development and Identification of a Key Regulatory Gene in Botrytis cinerea" Journal of Fungi 11, no. 1: 16. https://doi.org/10.3390/jof11010016

APA Style

Tang, M., Wang, K., Zhang, P., Hou, J., Yu, X., Wang, H., Wang, Y., & Li, G. (2025). Establishment of a Mutant Library for Infection Cushion Development and Identification of a Key Regulatory Gene in Botrytis cinerea. Journal of Fungi, 11(1), 16. https://doi.org/10.3390/jof11010016

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