Production and New Extraction Method of Polyketide Red Pigments Produced by Ascomycetous Fungi from Terrestrial and Marine Habitats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains
2.2. Fermentation and Biomass Production
2.3. Quantitative Colorimetric Analysis of Extracellular Extracts
2.4. UV-Visible Spectrophotometry and Extracellular Polyketide Metabolites Quantification
2.5. New Extraction Method of Intracellular Polyketide Pigments
2.6. Absorbance and HPLC-DAD Analyses
2.7. UHPLC-HRMS Analyses
3. Results
3.1. Biomass and Polyketide Extrolites Production Capacities under Various Growth Conditions
3.2. New Extraction Procedure and Nature of the Polyketide Red Pigments Produced by Talaromyces spp. Marine Isolate and P. purpurogenum rubisclerotium Terrestrial Isolate
4. Discussion
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Fungal Strain | Broth | Biomass | Extracellular Pigments | a-Value (CIELab) | Intracellular Pigments Extracted | |
---|---|---|---|---|---|---|
(g/L) | (meqv. Carmine/L) | (Red Color) | (meqv/g Biomass) or (meqv/L Culture) | |||
30570 | in DMD | 6.2–7.1 | 18–31 | 10.8–13.4 | 23.8–24.7 | 148–176 |
in PDB | 5.0–5.5 | 208–278 | 63.8–65.8 | 49.4–69.8 | 247–384 | |
in YCD | 1.6–2.0 | - | 5.3–5.6 | 44.3–48.5 | 71–97 | |
4464 | in DMD | 8.4–8.5 | 1–189 | 0.7 – 0.9 | 57.9–83.2 | 487–707 |
in PDB | 1.0–1.3 | 685–1118 | 49.9–51.5 | 69.2–116.0 | 90–116 | |
in YCD | 1.6–1.7 | 666–680 | 42.8–43.5 | 22.5–71.2 | 36–121 |
Compound No. R.t. (mn) | λmax (nm) | Solvent | Tentative Identification | Mol. Peak (m/z) | |
---|---|---|---|---|---|
IE of Talaromyces spp. (305_70) | |||||
1 | 1.71 | 215, 244, 276, 418, 514, 524 | water | n.i. | n.d. |
2 | 26.07 | 246, 276, 425, 512 | MeOH | n.i. | 488 [M + H]+ |
3 | 29.60 | 245, 274, 421, 518 | MeOH | Glycylrubropunctatin | 416 [M + H]+ |
4 | 30.97 | 245, 274, 424, 521 | MeOH | N-threoninerubropunctamine | 456 [M + H]+ |
5 | 32.66 | 218, 250, 287, 424, 546 | MeOH | n.i. | 498 [M + H]+ |
6 | 38.04 | 246, 273, 427, 521 | MeOH | N-glutarylrubropunctamine | 484 [M + H]+ |
7 | 43.95 | 245, 272, 424, 519 | MeOH | Monascorubramine | 381 [M + H]+ |
8 | 69.78 | 272, 282, 293 | MeOH | Ergosterol | 393 [M + H]+ |
IE of Penicillium purpurogenum rubisclerotium (4464) | |||||
8 | 69.78 | 272, 282, 293 | MeOH | Ergosterol | 393 [M + H]+ |
9 | 1.57 | 216, 292, 492 | MeOH | n.i. | n.d. |
10 | 22.05 | 262, 322, 430 | MeOH | n.i. | n.d. |
11 | 23.50 | 284, 389 | MeOH | n.i. | n.d. |
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Lebeau, J.; Venkatachalam, M.; Fouillaud, M.; Petit, T.; Vinale, F.; Dufossé, L.; Caro, Y. Production and New Extraction Method of Polyketide Red Pigments Produced by Ascomycetous Fungi from Terrestrial and Marine Habitats. J. Fungi 2017, 3, 34. https://doi.org/10.3390/jof3030034
Lebeau J, Venkatachalam M, Fouillaud M, Petit T, Vinale F, Dufossé L, Caro Y. Production and New Extraction Method of Polyketide Red Pigments Produced by Ascomycetous Fungi from Terrestrial and Marine Habitats. Journal of Fungi. 2017; 3(3):34. https://doi.org/10.3390/jof3030034
Chicago/Turabian StyleLebeau, Juliana, Mekala Venkatachalam, Mireille Fouillaud, Thomas Petit, Francesco Vinale, Laurent Dufossé, and Yanis Caro. 2017. "Production and New Extraction Method of Polyketide Red Pigments Produced by Ascomycetous Fungi from Terrestrial and Marine Habitats" Journal of Fungi 3, no. 3: 34. https://doi.org/10.3390/jof3030034