Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14
Abstract
:1. Introduction
2. Materials and Methods
2.1. Pathogen Inoculum
2.2. Pathogenicity Test
2.3. Genome Sequencing and Assembly
2.4. Genome Analysis, Comparative Genomics and Phylogenetics
3. Results & Discussion
3.1. R. solani AG1-IB Isolates 1/2/21 and O8/2 Appeared to Be Significantly Less Aggressive in Pathogenicity Tests in Comparison to AG1-IB 7/3/14
3.2. Genome Sequencing of the Less Aggressive R. solani AG1-IB Isolates 1/2/21 and O8/2
3.3. Differentiation of R. solani AG1-IB Isolates 1/2/21 and O8/2 Based on Contig-Length vs. Read-Count Analyses
3.4. Synteny Analysis, Gene Prediction, Annotation and Comparative Genomics Provide First Insights into the R. solani AG1-IB Pan-Genome
3.5. Phylogenetic Differentiation of R. solani AG1-IB Isolates Based on Shared Core Genes
4. Conclusions and Outlook
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Selected Groups | 1/2/21 | O8/2 | 7/3/14 |
---|---|---|---|
Celluloytic enzymes 1 | 35 | 32 | 46 |
Tyrosinases 1 | 0 | 1 | 3 |
Drug resistance proteins 1 | 0 | 0 | 1 |
Laccases 1 | 0 | 0 | 1 |
Cytochrome P450 1 | 7 | 11 | 10 |
Secondary Metabolite Regions | 1/2/21 | O8/2 | 7/3/14 |
---|---|---|---|
All | 17 | 15 | 16 |
Non-ribosomal peptide synthesis regions | 6 | 6 | 6 |
Alkaloid/terpene synthesis regions | 10 | 9 | 9 |
Siderophore regions | 1 | 0 | 1 |
ANI | 1/2/21 | O8/2 | 7/3/14 |
---|---|---|---|
1/2/21 | 100.00 | 99.12 | 98.92 |
O8/2 | 99.02 | 100.00 | 99.02 |
7/3/14 | 98.92 | 99.06 | 100.00 |
AAI | 1/2/21 | O8/2 | 7/3/14 |
---|---|---|---|
1/2/21 | 100.00 | 99.46 | 99.40 |
O8/2 | 99.46 | 100.00 | 99.46 |
7/3/14 | 99.40 | 99.46 | 100.00 |
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Wibberg, D.; Genzel, F.; Verwaaijen, B.; Blom, J.; Rupp, O.; Goesmann, A.; Zrenner, R.; Grosch, R.; Pühler, A.; Schlüter, A. Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14. J. Fungi 2021, 7, 832. https://doi.org/10.3390/jof7100832
Wibberg D, Genzel F, Verwaaijen B, Blom J, Rupp O, Goesmann A, Zrenner R, Grosch R, Pühler A, Schlüter A. Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14. Journal of Fungi. 2021; 7(10):832. https://doi.org/10.3390/jof7100832
Chicago/Turabian StyleWibberg, Daniel, Franziska Genzel, Bart Verwaaijen, Jochen Blom, Oliver Rupp, Alexander Goesmann, Rita Zrenner, Rita Grosch, Alfred Pühler, and Andreas Schlüter. 2021. "Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14" Journal of Fungi 7, no. 10: 832. https://doi.org/10.3390/jof7100832