Comparative Proteomic Analysis within the Developmental Stages of the Mushroom White Hypsizygus marmoreus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Culture Conditions and Acquisition of the White H. marmoreus Samples
2.2. Protein Extraction and Peptide Digestion
2.3. Label-Free LC-MS/MS Quantitative Proteomics Analysis
2.4. Peptides and Proteins Identification
2.5. Bioinformation Analysis
3. Results
3.1. Label-Free LC-MS/MS Quantitative Proteomics Analysis for the Six Groups of the White H. marmoreus Samples and the Correlation among the Six Groups of Samples
3.2. Six Groups of Samples Intersection and Quantitative Visualization of DEPs
3.3. GO Analysis for the DEPs in Each Group to Molecular Function (MF), Cellular Component (CC), and Biological Process (BP) Categories
3.4. KEGG Pathway Enrichment Analysis of DEPs from Mycelium to the Mature Fruiting Body
4. Discussion
4.1. The Proteome and Transcriptome of H. marmoreus Exhibit the Same Major Enriched Pathways and the Enriched Pathways Are Similar among Some Different Mushrooms
4.2. MAPK and cAMP Signaling Pathways Play Vital Roles in the Growth and Development Process
4.3. Identified CAZyme Families and Their Functions in the Growth and Development Process
4.4. Important TFs and Their Functions in the Growth and Development Process
4.5. HSPs and Their Functions in the Growth and Development Process
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yang, X.; Lin, R.; Xu, K.; Guo, L.; Yu, H. Comparative Proteomic Analysis within the Developmental Stages of the Mushroom White Hypsizygus marmoreus. J. Fungi 2021, 7, 1064. https://doi.org/10.3390/jof7121064
Yang X, Lin R, Xu K, Guo L, Yu H. Comparative Proteomic Analysis within the Developmental Stages of the Mushroom White Hypsizygus marmoreus. Journal of Fungi. 2021; 7(12):1064. https://doi.org/10.3390/jof7121064
Chicago/Turabian StyleYang, Xiuqing, Rongmei Lin, Kang Xu, Lizhong Guo, and Hao Yu. 2021. "Comparative Proteomic Analysis within the Developmental Stages of the Mushroom White Hypsizygus marmoreus" Journal of Fungi 7, no. 12: 1064. https://doi.org/10.3390/jof7121064