Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling Area
2.2. Isolation and Identification of Culturable Fungi
2.3. Identification of Fungal Strains
2.4. DNA Extraction and Metabarcoding Sequencing
2.5. Bioinformatics Analysis and Downstream Analysis
3. Results
3.1. Occurrence of Black Fungi in the Antarctic Endolithic Communities through Culture-Dependent Approach and Amplicon Sequencing
3.2. Effect of Sun Exposure
3.3. Effects of Altitude on Black Fungi Relative Abundance
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Selbmann, L.; Stoppiello, G.A.; Onofri, S.; Stajich, J.E.; Coleine, C. Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities. J. Fungi 2021, 7, 213. https://doi.org/10.3390/jof7030213
Selbmann L, Stoppiello GA, Onofri S, Stajich JE, Coleine C. Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities. Journal of Fungi. 2021; 7(3):213. https://doi.org/10.3390/jof7030213
Chicago/Turabian StyleSelbmann, Laura, Gerardo A. Stoppiello, Silvano Onofri, Jason E. Stajich, and Claudia Coleine. 2021. "Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities" Journal of Fungi 7, no. 3: 213. https://doi.org/10.3390/jof7030213