Fungal Jasmonate as a Novel Morphogenetic Signal for Pathogenesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains, Growth Conditions and Genetic Transformation
2.2. Nucleic Acid Manipulations
2.3. Generation of Constructs for Gene Deletions or Epitope Tagging
2.4. Appressorial Assays and Chemical Complementation Analyses
2.5. Protein Isolation and Western Blot Analysis
2.6. Chemical Analysis (Liquid Chromatography-Mass Spectrometry)
2.7. Nitro Blue Tetrazolium Staining
2.8. Live Cell Microscopy and Image Analyses
2.9. Plant Cultivar and Pathogenicity Assays
3. Results
3.1. Fungal Jasmonate Is Essential for Timely Cessation of Germ Tube Growth during Pathogenic Differentiation in M. oryzae
3.2. Opr1 Is Required for JA Biosynthesis in M. oryzae
3.3. Subcellular Localization of GFP-Opr1 in M. oryzae
3.4. Opr1/JA Signaling Pathway Is Essential for Proper Initiation of Appressorium Formation in M. oryzae
3.5. Functional Dependency and Crosstalk between JA and Cyclic AMP Signalling during Pathogenic Development in M. oryzae
3.6. Intrinsic Jasmonic Acid Modulates Redox Signaling in M. oryzae
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, Y.; Pagac, M.; Yang, F.; Patkar, R.N.; Naqvi, N.I. Fungal Jasmonate as a Novel Morphogenetic Signal for Pathogenesis. J. Fungi 2021, 7, 693. https://doi.org/10.3390/jof7090693
Liu Y, Pagac M, Yang F, Patkar RN, Naqvi NI. Fungal Jasmonate as a Novel Morphogenetic Signal for Pathogenesis. Journal of Fungi. 2021; 7(9):693. https://doi.org/10.3390/jof7090693
Chicago/Turabian StyleLiu, Yingyao, Martin Pagac, Fan Yang, Rajesh N. Patkar, and Naweed I. Naqvi. 2021. "Fungal Jasmonate as a Novel Morphogenetic Signal for Pathogenesis" Journal of Fungi 7, no. 9: 693. https://doi.org/10.3390/jof7090693