The Change in Fatty Acids and Sugars Reveals the Association between Trifoliate Orange and Endophytic Fungi
Abstract
:1. Introduction
2. Materials and Methods
2.1. Endophytic Fungi
2.2. Experimental Setup
2.3. Measurement of Root Fungal Colonization and Plant Biomass
2.4. Measurement of Sugars and FA Contents
2.5. Relative Expression of Root FA Desaturase Genes
2.6. Data Analysis
3. Results
3.1. Root Colonization after Inoculation of Endophytic Fungi
3.2. Effects of Endophytic Fungi on Biomass Production
3.3. Effects of Endophytic Fungi on Sugar Concentrations of Leaves and Roots
3.4. Effects of Endophytic Fungi on FAs Contents in Roots
3.5. Effects of Endophytic Fungi on Relative Expression of FA Desaturase Genes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Genes | Gene IDs | Sequence (5’→3’)-Forward | Sequence (5’→3’)-Reverse |
---|---|---|---|
PtFAD2 | Orange1.1t02241 | AGGAGGCAAGAGTGGAGGATAAGG | GGAGCAGGTGGACGAATGTCTG |
PtFAD6 | Cs8g17450 | CTGCACGGAGATACAGCTTGGC | GGAATGTGAGGAGCCGTATGATGC |
PtΔ9 | orange1.1t03533 | TGCCTGCTCACTTGATGTACGATG | CTCCTCCAGCCTTCTGATTCTTGC |
PtΔ15 | Cs6g08600 | CAAGAACTGGTCTAGCAGCCTCAG | ATGTGGCTGGACCTTGTGACTTAC |
β-Actin | Cs1g05000 | CCGACCGTATGAGCAAGGAAA | TTCCTGTGGACAATGGATGGA |
Treatments | Sucrose | Glucose | Fructose | |||
---|---|---|---|---|---|---|
Leaves | Roots | Leaves | Roots | Leaves | Roots | |
Control | 58.86 ± 6.57 c | 25.54 ± 4.61 b | 66.30 ± 6.41 c | 86.16 ± 27.40 b | 112.81 ± 4.96 c | 114.80 ± 8.03 b |
Fm | 73.47 ± 4.77 b | 34.95 ± 7.82 b | 155.45 ± 3.48 ab | 125.42 ± 7.68 a | 125.76 ± 2.61 ab | 138.09 ± 1.96 a |
Pi | 83.11 ± 3.63 a | 57.25 ± 9.56 a | 171.42 ± 34.44 a | 141.84 ± 9.15 a | 130.50 ± 0.40 a | 141.34 ± 5.56 a |
Fm + Pi | 66.55 ± 2.43 b | 52.01 ± 11.68 a | 85.43 ± 7.88 c | 129.45 ± 12.77 a | 123.56 ± 3.13 b | 132.53 ± 7.15 a |
FA Types | FA Species | Treatments (μg/g FW) | |||
---|---|---|---|---|---|
Control | Pi | Fm | Fm + Pi | ||
Saturated FAs | Methyl hexanoate (C6:0) | 1.47 ± 0.48 b | 2.13 ± 0.30 a | 1.96 ± 0.17 ab | 1.63 ± 0.09 ab |
Methyl octanoate (C8:0) | 0.53 ± 0.05 b | 0.92 ± 0.21 a | 0.50 ± 0.02 b | 0.45 ± 0.01 b | |
Methyl decanoate (C10:0) | 1.18 ± 0.17 ab | 1.44 ± 0.20 a | 1.04 ± 0.08 bc | 0.89 ± 0.06 c | |
Methyl undecanoate (C11:0) | ND | ND | ND | ND | |
Methyl laurate (C12:0) | 8.80 ± 0.57 a | 9.56 ± 0.69 a | 8.06 ± 1.27 ab | 7.31 ± 0.43 b | |
Methyl tridecanoate (C13:0) | 1.32 ± 0.14 b | 1.48 ± 0.19 a | 1.39 ± 0.07 ab | 1.30 ± 0.14 ab | |
Methyl myristate (C14:0) | 18.14 ± 0.90 c | 23.80 ± 1.70 a | 21.15 ± 1.10 b | 17.76 ± 1.18 c | |
Methyl pentadecanoate (C15:0) | 11.38 ± 0.90 a | 13.86 ± 0.63 a | 12.84 ± 1.51 a | 11.30 ± 1.85 a | |
Methyl palmitate (C16:0) | 1210.76 ± 114.10 a | 1379.62 ± 172.93 a | 1274.72 ± 14.76 a | 1227.51 ± 22.79 a | |
Methyl heptadecanoate (C17:0) | 16.98 ± 2.19 a | 16.91 ± 1.59 a | 17.07 ± 3.18 a | 15.93 ± 2.23 a | |
Methyl stearate (C18:0) | 767.37 ± 124.56 a | 823.26 ± 28.44 a | 791.65 ± 22.28 a | 757.79 ± 30.67 a | |
Methyl arachidate (C20:0) | 12.89 ± 2.34 ab | 13.69 ± 0.82 a | 12.01 ± 0.13 ab | 10.80 ± 0.64 b | |
Methyl heneicosadienoate (C21:0) | 2.11 ± 0.19 a | 2.14 ± 0.14 a | 1.92 ± 0.20 ab | 1.72 ± 0.22 b | |
Methyl behenate (C22:0) | 13.62 ± 1.36 ab | 15.82 ± 1.68 a | 13.46 ± 1.90 ab | 12.02 ± 1.16 b | |
Methyl tricosanoate (C23:0) | 13.22 ± 1.43 ab | 15.54 ± 1.74 a | 12.16 ± 1.84 b | 10.70 ± 1.01 b | |
Methyl lignocerate (C24:0) | 26.45 ± 3.96 b | 36.00 ± 5.54 a | 28.76 ± 5.06 ab | 26.88 ± 3.24 b | |
Unsaturated FAs | Methyl myristoleate (C14:1) | ND | ND | ND | ND |
Methyl pentadecenoate (C15:1) | ND | ND | ND | ND | |
Methyl palmitoleate (C16:1) | 9.97 ± 2.21 a | 9.15 ± 1.83 a | 7.61 ± 2.99 a | 8.90 ± 1.47 a | |
Methyl heptadecenoate (C17:1) | 8.45 ± 1.77 a | 9.09 ± 0.32 a | 9.59 ± 0.34 a | 8.47 ± 0.15 a | |
Methyl oleate (C18:1) | 175.21 ± 20.81 a | 162.46 ± 25.39 ab | 158.08 ± 2.69 ab | 125.60 ± 20.85 b | |
Methyl linoleate (C18:2) | 548.39 ± 40.90 a | 542.91 ± 42.95 a | 490.88 ± 90.61 a | 459.15 ± 29.9 a | |
Methyl gamma-Linolenate (C18:3N6) | 6.51 ± 0.56 b | 10.66 ± 1.35 a | 7.49 ± 2.45 b | 8.24 ± 0.39 ab | |
Methyl linolenate (C18:3N3) | 149.23 ± 21.49 a | 159.62 ± 16.98 a | 133.37 ± 13.39 ab | 115.82 ± 6.63 b | |
cis-11-Eicosenoic acid methyl ester (C20:1) | 8.68 ± 0.82 ab | 9.90 ± 1.01 a | 8.96 ± 0.13 ab | 7.84 ± 0.25 b | |
cis-11,14-Eicosadienoic acid methyl ester (C20:2) | 2.31 ± 0.27 b | 3.48 ± 0.77 a | 2.79 ± 0.46 ab | 2.75 ± 0.34 ab | |
Cis-11,14,-Eicosatrienotic acid methyl ester (C20:3N6) | 15.61 ± 0.78 b | 31.62 ± 6.99 a | 18.98 ± 2.30 b | 19.13 ± 1.19 b | |
Arachidonate (C20:4N6) | 34.44 ± 2.96 b | 46.50 ± 6.40 a | 27.85 ± 6.81 b | 28.58 ± 0.01 b | |
Cis-11,14,17-Eicosatrienoate acid methyl ester (C20:3N3) | 0.81 ± 0.08 b | 1.03 ± 0.07 a | 0.92 ± 0.05 ab | 0.82 ± 0.01 b | |
Cis-5,8,11,14,17-eicosapentaenoate tic acid methyl ester (C20:5N3) | 1.56 ± 0.43 c | 4.53 ± 0.33 b | 4.02 ± 1.58 b | 6.721 ± 1.58 a | |
Methyl erucate (C22:1N9) | 8.68 ± 1.90 b | 12.23 ± 0.86 a | 11.22 ± 1.72 ab | 10.99 ± 2.15 ab | |
Cis-13,16-Docosadienotic acid methyl ester (C22:2) | ND | ND | ND | ND | |
Cis-4,7,10,13,16,19-Docosahexaenotic acid methyl ester (C22:6N3) | ND | ND | ND | ND | |
Methyl cis-15-tetracosenoate (C24:1) | 15.84 ± 2.56 b | 26.73 ± 6.12 a | 17.76 ± 2.96 b | 18.05 ± 1.99 b |
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Meng, L.-L.; Liu, R.-C.; Yang, L.; Zou, Y.-N.; Srivastava, A.K.; Kuča, K.; Hashem, A.; Abd_Allah, E.F.; Giri, B.; Wu, Q.-S. The Change in Fatty Acids and Sugars Reveals the Association between Trifoliate Orange and Endophytic Fungi. J. Fungi 2021, 7, 716. https://doi.org/10.3390/jof7090716
Meng L-L, Liu R-C, Yang L, Zou Y-N, Srivastava AK, Kuča K, Hashem A, Abd_Allah EF, Giri B, Wu Q-S. The Change in Fatty Acids and Sugars Reveals the Association between Trifoliate Orange and Endophytic Fungi. Journal of Fungi. 2021; 7(9):716. https://doi.org/10.3390/jof7090716
Chicago/Turabian StyleMeng, Lu-Lu, Rui-Cheng Liu, Liu Yang, Ying-Ning Zou, Anoop Kumar Srivastava, Kamil Kuča, Abeer Hashem, Elsayed Fathi Abd_Allah, Bhoopander Giri, and Qiang-Sheng Wu. 2021. "The Change in Fatty Acids and Sugars Reveals the Association between Trifoliate Orange and Endophytic Fungi" Journal of Fungi 7, no. 9: 716. https://doi.org/10.3390/jof7090716