Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Identification of B. velezensis TSA 32-1
2.2. Phylogenetic and Statistical Analysis
2.3. Enzymatic Activity Analysis
2.3.1. Cellulase Activity
2.3.2. Lysozyme Activity
2.3.3. Protease Activity
2.4. Antifungal Analysis
2.5. Pathogen Inoculum Production and Disease Evaluation
2.5.1. Preparation of Bacillus Strains
2.5.2. Preparation of Seeds
2.5.3. Preparation of Pathogenic Fungus
2.5.4. Seed Germination Rate of Treatments
2.5.5. Disease Suppression Effect of Seed Treatments
2.5.6. DNA Extraction and Whole-Genome Sequencing
2.5.7. Annotation
2.6. Antifungal Metabolite Extraction and Analysis
2.6.1. Preparation of Culture Extract
2.6.2. Ultra-High Performance (UP) LC-QTOF-Mass Spectrometry (MS) Analysis of Metabolites
3. Results
3.1. Identification of Bacterial Strains
3.2. Plant Disease Biocontrol Activity
3.2.1. Enzyme Activity of Isolated Strains
3.2.2. In Vitro Antifungal Effects against Phytopathogenic Fungi
3.2.3. Plant Protection Effects of Seed Treatments with TSA32-1
3.3. Genome Feature of B. velezensis TSA32-1
3.4. Antifungal Metabolite of B. velezensis TSA32-1
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compound | Synthetase Type | Genes | Size (kb) | Bioactivity |
---|---|---|---|---|
Surfactin | NRPS | srfAA, AB, AC, AD | 26.1 | multiple |
Bacilysin | NRPS | ywfA, bacA, B, C, D, E, F, ywfH | 6.7 | antibacterial |
Plipastatin | NRPS | ppsA, ppsC, ppsD, ppsE | 37.6 | antifungal |
Bacillaene | PKS-NRPS | baeB, C, D, E, acpK, baeG, H, I, J, L, M, N, R, S baeC, L, M, ppsC, baeL, M baeC, G, I, L, M | 71.7 43.3 64.6 | antibacterial |
Mycosubtilin | PKS-NRPS | mycA, B, C | 35.9 | antifungal |
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Kim, J.-A.; Song, J.-S.; Kim, P.I.; Kim, D.-H.; Kim, Y. Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi. J. Fungi 2022, 8, 1053. https://doi.org/10.3390/jof8101053
Kim J-A, Song J-S, Kim PI, Kim D-H, Kim Y. Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi. Journal of Fungi. 2022; 8(10):1053. https://doi.org/10.3390/jof8101053
Chicago/Turabian StyleKim, Jung-Ae, Jeong-Sup Song, Pyoung Il Kim, Dae-Hyuk Kim, and Yangseon Kim. 2022. "Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi" Journal of Fungi 8, no. 10: 1053. https://doi.org/10.3390/jof8101053