Secretome Profiling by Proteogenomic Analysis Shows Species-Specific, Temperature-Dependent, and Putative Virulence Proteins of Pythium insidiosum
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microorganism and Protein Extraction
2.2. Protein Digestion and Liquid Chromatography–Tandem Mass Spectrometry
2.3. Proteomic Data Analysis
2.4. Bioinformatic Analysis
2.5. Data Availability
3. Results
3.1. Identification and Validation of P. insidiosum Secretome
3.2. Functional Annotation of P. insidiosum’s Secretome
3.3. Effect of the Temperature on the Growth of the Organism and Expression of the Secretory/Extracellular Proteins
P. insidiosum Protein ID | Functional Annotation a | Average Protein Abundance at 37 °C (emPAI b) | Average Protein Abundance at 25 °C (emPAI) | Relative Protein Abundance (37:25 °C) | Accession Number |
---|---|---|---|---|---|
PINS01640001A | Cyclophilin A | 50.18 | - | - | GAY01457.1 |
PINS00590035A | Elicitin-like protein c,d | 9.94 | - | - | GAX97068.1 |
PINS00010057C | Hypothetical protein c,d | 6.69 | - | - | GAX92118.1 |
PINS00010061C | Hypothetical protein c,d | 3.50 | - | - | GAX92125.1 |
PINS01530002A | Hypothetical protein d | 1.79 | - | - | GAY01151.1 |
PINS00660046A | Hypothetical protein c,d | 1.69 | - | - | GAX97463.1 |
PINS00380039A | ATP synthase subunit beta, mitochondrial | 1.44 | - | - | GAX95749.1 |
PINS00660004A | Glycerophosphoryl diester phosphodiesterase | 2.34 | 0.22 | 10.61 | GAX97472.1 |
PINS02090002A | Elicitin-like protein c,d | 71.97 | 7.82 | 9.21 | GAY02672.1 |
PINS01630040A | Hypothetical protein c,d | 1.27 | 0.15 | 8.43 | GAY01431.1 |
PINS02430017C | Cofilin/tropomyosin-type actin-binding protein | 0.11 | 0.02 | 7.33 | GAY03393.1 |
PINS00560010C | Cofilin/tropomyosin-type actin-binding protein | 0.14 | 0.02 | 7.00 | GAX96878.1 |
PINS00420001A | Malate dehydrogenase, NAD-dependent | 2.00 | 0.30 | 6.76 | GAX96042.1 |
PINS06030002B | Glycoside hydrolase d | 0.11 | 0.02 | 5.25 | GAY06261.1 |
3.4. Putative Pathogenicity Proteins in P. insidiosum’s Secretome
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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VFID | MvirDB-Defined Virulence Factors | Organisms | Virulence Types | P. insidiosum Protein ID | Accession Number | Identity (%) | E-Value |
---|---|---|---|---|---|---|---|
26381 | Heat shock protein 70 | Cryptosporidium parvum | Virulence protein | PINS00530003B | GAX96752.1 | 71.24 | 0 |
26468 | Organellar heat shock protein | Eimeria tenella | Virulence protein | PINS01150027A | GAX99732.1 | 65.0 | 0 |
12025 | Urease | Oryza sativa | Virulence protein | PINS00920015C a | GAX98700.1 | 64.5 | 0 |
15128 | Chaperone protein ClpB | Francisella tularensis | Virulence protein | PINS01620006A | GAY01393.1 | 55.1 | 0 |
26434 | Carbamoyl phosphate synthetase II | Toxoplasma gondii | Virulence protein | PINS04750002A | GAY05754.1 | 53.3 | 0 |
11169 | Tripeptidyl-peptidase 2 | Mus musculus | Protein toxin | PINS01420022C | GAY00792.1 | 34.8 | 0 |
26447 | Heat shock protein 70 | Toxoplasma gondii | Virulence protein | PINS00020096A | GAX92393.1 | 57.6 | 0 |
26455 | Eukaryotic translation initiation factor 4A | Toxoplasma gondii | Virulence protein | PINS00130063A | GAX93656.1 | 72.9 | 1 × 10−173 |
8350 | Chaperonin GroEL | Legionella pneumophila | Virulence protein | PINS00020065A | GAX92364.1 | 57.0 | 3 × 10−159 |
8728 | Phosphoglucomutase | Brucella melitensis | Virulence protein | PINS00550030A | GAX96838.1 | 50.4 | 7 × 10−158 |
12413 | Transcription factor site-1 protease | Homo sapiens | Protein toxin | PINS02120014C | GAY02722.1 | 40.2 | 5 × 10−142 |
7573 | Nonribosomal peptide synthetase Dhbf | Bacillus anthracis | Virulence protein | PINS01020025B | GAX99146.1 | 29.0 | 1 × 10−113 |
20164 | Asparaginyl-tRNA synthetase | Salmonella enterica | Pathogenicity island | PINS00170005A | GAX93976.1 | 46.7 | 6 × 10−113 |
13372 | Pyruvate kinase | Salmonella typhimurium | Virulence protein | PINS00750004A | GAX97930.1 | 45.9 | 3 × 10−107 |
26400 | cGMP dependent protein kinase | Toxoplasma gondii | Virulence protein | PINS00410016C | GAX95955.1 | 33.0 | 6 × 10−105 |
26980 | AP65-1 adhesin | Trichomonas vaginalis | Virulence protein | PINS01980002A | GAY02392.1 | 37.9 | 5 × 10−101 |
26382 | Heat shock protein 90 | Cryptosporidium parvum | Virulence protein | PINS00460042A | GAX96321.1 | 42.1 | 3 × 10−100 |
26459 | Peroxisomal catalase | Toxoplasma gondii | Virulence protein | PINS00770005C | GAX98049.1 | 40.6 | 2 × 10−92 |
20579 | Recombination factor protein RarA | Salmonella enterica | Pathogenicity island | PINS00070081C | GAX93094.1 | 44.8 | 5 × 10−91 |
14026 | Lysil-tRNA synthetase LysU | Escherichia coli | Virulence protein | PINS00380044C | GAX95759.1 | 38.5 | 5 × 10−91 |
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Krajaejun, T.; Rujirawat, T.; Lohnoo, T.; Yingyong, W.; Sae-Chew, P.; Reamtong, O.; Kittichotirat, W.; Patumcharoenpol, P. Secretome Profiling by Proteogenomic Analysis Shows Species-Specific, Temperature-Dependent, and Putative Virulence Proteins of Pythium insidiosum. J. Fungi 2022, 8, 527. https://doi.org/10.3390/jof8050527
Krajaejun T, Rujirawat T, Lohnoo T, Yingyong W, Sae-Chew P, Reamtong O, Kittichotirat W, Patumcharoenpol P. Secretome Profiling by Proteogenomic Analysis Shows Species-Specific, Temperature-Dependent, and Putative Virulence Proteins of Pythium insidiosum. Journal of Fungi. 2022; 8(5):527. https://doi.org/10.3390/jof8050527
Chicago/Turabian StyleKrajaejun, Theerapong, Thidarat Rujirawat, Tassanee Lohnoo, Wanta Yingyong, Pattarana Sae-Chew, Onrapak Reamtong, Weerayuth Kittichotirat, and Preecha Patumcharoenpol. 2022. "Secretome Profiling by Proteogenomic Analysis Shows Species-Specific, Temperature-Dependent, and Putative Virulence Proteins of Pythium insidiosum" Journal of Fungi 8, no. 5: 527. https://doi.org/10.3390/jof8050527