Identification and Functional Characterization of Adenosine Deaminase in Mucor circinelloides: A Novel Potential Regulator of Nitrogen Utilization and Lipid Biosynthesis
Abstract
:1. Introduction
2. Results
2.1. Identification of Gene Coding for the ADA in M. circinelloides
2.2. Generation of Ada Overexpressing and Knockout Transformants of M. circinelloides
2.3. Analysis of Ada Expression in the Transformants
2.4. Ada Regulated Nitrogen Metabolism and Affected Cell Growth and Lipid Accumulation
2.5. Impacts of Ada Gene Manipulation on the Expression Levels of the Key Genes for Fatty Acid Biosynthesis
3. Discussion
4. Materials and Methods
4.1. Strains, Transformation, and Fermentation Conditions
4.2. Identification and Bioinformatics Analysis of ADA Gene in M. circinelloides
4.3. Plasmid Construction
4.4. Biochemical Analysis of the Fermentation Process
4.5. Determination of Lipid Accumulation in Transformants
4.6. RNA Isolation and Gene Expression Analysis by qRT-PCR
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, S.; Yang, J.; Mohamed, H.; Wang, X.; Pang, S.; Wu, C.; López-García, S.; Song, Y. Identification and Functional Characterization of Adenosine Deaminase in Mucor circinelloides: A Novel Potential Regulator of Nitrogen Utilization and Lipid Biosynthesis. J. Fungi 2022, 8, 774. https://doi.org/10.3390/jof8080774
Li S, Yang J, Mohamed H, Wang X, Pang S, Wu C, López-García S, Song Y. Identification and Functional Characterization of Adenosine Deaminase in Mucor circinelloides: A Novel Potential Regulator of Nitrogen Utilization and Lipid Biosynthesis. Journal of Fungi. 2022; 8(8):774. https://doi.org/10.3390/jof8080774
Chicago/Turabian StyleLi, Shaoqi, Junhuan Yang, Hassan Mohamed, Xiuwen Wang, Shuxian Pang, Chen Wu, Sergio López-García, and Yuanda Song. 2022. "Identification and Functional Characterization of Adenosine Deaminase in Mucor circinelloides: A Novel Potential Regulator of Nitrogen Utilization and Lipid Biosynthesis" Journal of Fungi 8, no. 8: 774. https://doi.org/10.3390/jof8080774