Functional Roles of LaeA-like Genes in Fungal Growth, Cellulase Activity, and Secondary Metabolism in Pleurotus ostreatus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microbial Strains and Culture Conditions
2.2. Gene Cloning and Sequence Analysis
2.3. Construction of PoLaeA-RNAi Plasmids and Strains
2.4. Measurement of Mycelial Growth and Biomass
2.5. Measurement of the Intracellular Reactive Oxygen Species (ROS) Level
2.6. Enzymatic Activity Determination
2.7. Measurement of the Cytosolic Ca2+ Content
2.8. Determination of Intracellular Polysaccharide Content
2.9. Analysis of Gene Expression with qRT–PCR
2.10. Data Analysis
3. Results
3.1. LaeA-like Genes in P. ostreatus
3.2. Expression of LaeA-like Genes during Different Developmental Stages of P. ostreatus
3.3. Construction of P. ostreatus LaeA-Silenced Strains
3.4. LaeA-like Genes Are Required for the Fungal Growth of P. ostreatus
3.5. Silencing PoLaeA1 Decreased the Intracellular ROS Levels of P. ostreatus
3.6. Silencing PoLaeA2 and PoLaeA3 Reduced the Cytosolic Ca2+ Content of P. ostreatus
3.7. The IPS Contents Were Decreased in PoLaeA1-Silenced Strains and Could Be Recovered by H2O2
3.8. The Cytosolic Ca2+ Content Affected the Regulation of Cellulase Activities by PoLaeA2 and PoLaeA3
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhang, G.; Yan, P.; Leng, D.; Shang, L.; Zhang, C.; Wu, Z.; Wang, Z. Functional Roles of LaeA-like Genes in Fungal Growth, Cellulase Activity, and Secondary Metabolism in Pleurotus ostreatus. J. Fungi 2022, 8, 902. https://doi.org/10.3390/jof8090902
Zhang G, Yan P, Leng D, Shang L, Zhang C, Wu Z, Wang Z. Functional Roles of LaeA-like Genes in Fungal Growth, Cellulase Activity, and Secondary Metabolism in Pleurotus ostreatus. Journal of Fungi. 2022; 8(9):902. https://doi.org/10.3390/jof8090902
Chicago/Turabian StyleZhang, Guang, Peng Yan, Doudou Leng, Li Shang, Chaohui Zhang, Zhongwei Wu, and Zhenhe Wang. 2022. "Functional Roles of LaeA-like Genes in Fungal Growth, Cellulase Activity, and Secondary Metabolism in Pleurotus ostreatus" Journal of Fungi 8, no. 9: 902. https://doi.org/10.3390/jof8090902