Metabarcoding and Metabolome Analyses Reveal Mechanisms of Leymus chinensis Growth Promotion by Fairy Ring of Leucocalocybe mongolica
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Metabarcoding Survey
2.2.2. Widely Targeted Metabolome Analysis
2.2.3. Correlation Analysis
3. Results
3.1. Microbial Diversity
3.1.1. Metabarcoding Sequencing
3.1.2. Microbial Alpha Diversity
3.1.3. Key Microbial Taxon
3.2. Metabolome Analysis
3.2.1. Metabolite Composition
3.2.2. Differential Metabolites
3.2.3. Metabolite Pathway Analysis
3.3. Correlation Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample ID | Bacteria | Fungi | ||||
---|---|---|---|---|---|---|
Clean Tags | Average Length (nt) | OTU Number | Clean Tags | Average Length (nt) | OTU Number | |
Leaf.CK1 | 104,742 | 374 | 362 | 84,184 | 233 | 485 |
Leaf.CK2 | 80,975 | 374 | 277 | 81,380 | 234 | 515 |
Leaf.CK3 | 98,878 | 374 | 283 | 84,101 | 239 | 500 |
Leaf.FR1 | 95,560 | 374 | 365 | 74,920 | 237 | 1038 |
Leaf.FR2 | 96,290 | 375 | 418 | 77,076 | 235 | 951 |
Leaf.FR3 | 91,267 | 375 | 459 | 82,657 | 237 | 868 |
Root.CK1 | 90,050 | 382 | 964 | 85,607 | 267 | 402 |
Root.CK2 | 92,448 | 384 | 1058 | 76,933 | 261 | 407 |
Root.CK3 | 95,095 | 392 | 988 | 79,489 | 264 | 504 |
Root.FR1 | 77,247 | 381 | 906 | 75,696 | 279 | 706 |
Root.FR2 | 85,075 | 379 | 803 | 68,124 | 267 | 895 |
Root.FR3 | 97,973 | 378 | 803 | 87,868 | 272 | 714 |
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Duan, M.; Lu, J.; Yang, W.; Lu, M.; Wang, J.; Li, S.; Chen, Y.; Hu, L.; Wang, L. Metabarcoding and Metabolome Analyses Reveal Mechanisms of Leymus chinensis Growth Promotion by Fairy Ring of Leucocalocybe mongolica. J. Fungi 2022, 8, 944. https://doi.org/10.3390/jof8090944
Duan M, Lu J, Yang W, Lu M, Wang J, Li S, Chen Y, Hu L, Wang L. Metabarcoding and Metabolome Analyses Reveal Mechanisms of Leymus chinensis Growth Promotion by Fairy Ring of Leucocalocybe mongolica. Journal of Fungi. 2022; 8(9):944. https://doi.org/10.3390/jof8090944
Chicago/Turabian StyleDuan, Mingzheng, Jia Lu, Wenjing Yang, Meiling Lu, Jibin Wang, Suli Li, Yiting Chen, Lihua Hu, and Lingqiang Wang. 2022. "Metabarcoding and Metabolome Analyses Reveal Mechanisms of Leymus chinensis Growth Promotion by Fairy Ring of Leucocalocybe mongolica" Journal of Fungi 8, no. 9: 944. https://doi.org/10.3390/jof8090944